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Self-Disinfecting Paints with the Natural Antimicrobial Substances: Colophony and Curcumin

The risk of infection arising from indirect sources—namely, contaminated surfaces—has been proved, particularly in healthcare facilities. In the attempt to minimize this problem, innumerable research projects involving the development of surfaces with self-disinfecting properties are being conducted...

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Autores principales: Querido, Micaela Machado, Paulo, Ivo, Hariharakrishnan, Sriram, Rocha, Daniel, Barbosa, Nuno, Galhano dos Santos, Rui, Bordado, João Moura, Teixeira, João Paulo, Pereira, Cristiana Costa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615116/
https://www.ncbi.nlm.nih.gov/pubmed/34827290
http://dx.doi.org/10.3390/antibiotics10111351
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author Querido, Micaela Machado
Paulo, Ivo
Hariharakrishnan, Sriram
Rocha, Daniel
Barbosa, Nuno
Galhano dos Santos, Rui
Bordado, João Moura
Teixeira, João Paulo
Pereira, Cristiana Costa
author_facet Querido, Micaela Machado
Paulo, Ivo
Hariharakrishnan, Sriram
Rocha, Daniel
Barbosa, Nuno
Galhano dos Santos, Rui
Bordado, João Moura
Teixeira, João Paulo
Pereira, Cristiana Costa
author_sort Querido, Micaela Machado
collection PubMed
description The risk of infection arising from indirect sources—namely, contaminated surfaces—has been proved, particularly in healthcare facilities. In the attempt to minimize this problem, innumerable research projects involving the development of surfaces with self-disinfecting properties are being conducted. In this work, wall-paints with self-disinfecting properties were developed with the scope of being applied in environments prone to contamination, such as those at healthcare settings. Our approach was to develop new paint formulations containing two natural plant-based products with known antimicrobial activity—colophony (CLF) and curcumin (CUR). The natural substances were separately incorporated on a commercial paint and their antibacterial activity was evaluated with several bacterial species following ISO 22196. To assess the paints’ safety, cytotoxicity tests were performed on HaCaT and A549 cell lines, using tests on extracts and direct contact tests, as suggested by the standardized protocol ISO 10993. In general, both paints containing CLF and CUR were able to reduce the bacterial growth after 24 h, compared with the control, the commercial unmodified paint. Colophony was even able to reduce the number of culturable bacteria by over 2 log for Staphylococcus aureus, Escherichia coli, and Bacillus cereus. Regarding the cytotoxicity tests performed (WST-1, NRU, and LDH), both formulations revealed promising results regardless of the methodology used.
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spelling pubmed-86151162021-11-26 Self-Disinfecting Paints with the Natural Antimicrobial Substances: Colophony and Curcumin Querido, Micaela Machado Paulo, Ivo Hariharakrishnan, Sriram Rocha, Daniel Barbosa, Nuno Galhano dos Santos, Rui Bordado, João Moura Teixeira, João Paulo Pereira, Cristiana Costa Antibiotics (Basel) Article The risk of infection arising from indirect sources—namely, contaminated surfaces—has been proved, particularly in healthcare facilities. In the attempt to minimize this problem, innumerable research projects involving the development of surfaces with self-disinfecting properties are being conducted. In this work, wall-paints with self-disinfecting properties were developed with the scope of being applied in environments prone to contamination, such as those at healthcare settings. Our approach was to develop new paint formulations containing two natural plant-based products with known antimicrobial activity—colophony (CLF) and curcumin (CUR). The natural substances were separately incorporated on a commercial paint and their antibacterial activity was evaluated with several bacterial species following ISO 22196. To assess the paints’ safety, cytotoxicity tests were performed on HaCaT and A549 cell lines, using tests on extracts and direct contact tests, as suggested by the standardized protocol ISO 10993. In general, both paints containing CLF and CUR were able to reduce the bacterial growth after 24 h, compared with the control, the commercial unmodified paint. Colophony was even able to reduce the number of culturable bacteria by over 2 log for Staphylococcus aureus, Escherichia coli, and Bacillus cereus. Regarding the cytotoxicity tests performed (WST-1, NRU, and LDH), both formulations revealed promising results regardless of the methodology used. MDPI 2021-11-05 /pmc/articles/PMC8615116/ /pubmed/34827290 http://dx.doi.org/10.3390/antibiotics10111351 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Querido, Micaela Machado
Paulo, Ivo
Hariharakrishnan, Sriram
Rocha, Daniel
Barbosa, Nuno
Galhano dos Santos, Rui
Bordado, João Moura
Teixeira, João Paulo
Pereira, Cristiana Costa
Self-Disinfecting Paints with the Natural Antimicrobial Substances: Colophony and Curcumin
title Self-Disinfecting Paints with the Natural Antimicrobial Substances: Colophony and Curcumin
title_full Self-Disinfecting Paints with the Natural Antimicrobial Substances: Colophony and Curcumin
title_fullStr Self-Disinfecting Paints with the Natural Antimicrobial Substances: Colophony and Curcumin
title_full_unstemmed Self-Disinfecting Paints with the Natural Antimicrobial Substances: Colophony and Curcumin
title_short Self-Disinfecting Paints with the Natural Antimicrobial Substances: Colophony and Curcumin
title_sort self-disinfecting paints with the natural antimicrobial substances: colophony and curcumin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615116/
https://www.ncbi.nlm.nih.gov/pubmed/34827290
http://dx.doi.org/10.3390/antibiotics10111351
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