Cargando…
Evaluation of Macular Retinal Vessels and Histological Changes in Two Cases of COVID-19
The purpose of this study was to assess vascular and histological alterations in two COVID-19 and three control post-mortem retinas. The macular areas of flat-mounted samples were processed for immunofluorescence. Lectin and collagen IV positive vessels were captured under confocal microscopy, and e...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615149/ https://www.ncbi.nlm.nih.gov/pubmed/34829775 http://dx.doi.org/10.3390/biomedicines9111546 |
_version_ | 1784604034899902464 |
---|---|
author | Hernandez, Maria González-Zamora, Jorge Recalde, Sergio Moreno-Orduña, Maite Bilbao-Malavé, Valentina Saenz de Viteri, Manuel Landecho, Manuel F. Fernandez-Robredo, Patricia García-Layana, Alfredo |
author_facet | Hernandez, Maria González-Zamora, Jorge Recalde, Sergio Moreno-Orduña, Maite Bilbao-Malavé, Valentina Saenz de Viteri, Manuel Landecho, Manuel F. Fernandez-Robredo, Patricia García-Layana, Alfredo |
author_sort | Hernandez, Maria |
collection | PubMed |
description | The purpose of this study was to assess vascular and histological alterations in two COVID-19 and three control post-mortem retinas. The macular areas of flat-mounted samples were processed for immunofluorescence. Lectin and collagen IV positive vessels were captured under confocal microscopy, and endothelium loss and tortuosity were analyzed. Expression of ACE2 (angiotensin-converting enzyme 2) (the receptor for SARS-CoV-2), Iba1 (ionized calcium-binding adaptor molecule 1) and GFAP (glial fibrillary acidic protein) were quantified in retinal sections. The number of lectin vessels in COVID-19 retinas decreased by 27% compared to the control (p < 0.01) and the tortuosity increased in COVID-19 retinas (7.3 ± 0.2) vs. control retinas (6.8 ± 0.07) (p < 0.05). Immunofluorescence analysis revealed an increase in ACE2 (2.3 ± 1.3 vs. 1.0 ± 0.1; p < 0.0001) and Iba1 expression (3.06 ± 0.6 vs. 1.0 ± 0.1; p < 0.01) in COVID-19 sections whereas no changes in GFAP were observed. Analysis of the COVID-19 macular retinal tissue suggested that endothelial cells are a preferential target of SARS-CoV-2 with subsequent changes through their ACE2 receptor expression and morphology. Thus, microglial activation was hyperactive when facing an ensuing immunological challenge after SARS-CoV-2 infection. |
format | Online Article Text |
id | pubmed-8615149 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86151492021-11-26 Evaluation of Macular Retinal Vessels and Histological Changes in Two Cases of COVID-19 Hernandez, Maria González-Zamora, Jorge Recalde, Sergio Moreno-Orduña, Maite Bilbao-Malavé, Valentina Saenz de Viteri, Manuel Landecho, Manuel F. Fernandez-Robredo, Patricia García-Layana, Alfredo Biomedicines Article The purpose of this study was to assess vascular and histological alterations in two COVID-19 and three control post-mortem retinas. The macular areas of flat-mounted samples were processed for immunofluorescence. Lectin and collagen IV positive vessels were captured under confocal microscopy, and endothelium loss and tortuosity were analyzed. Expression of ACE2 (angiotensin-converting enzyme 2) (the receptor for SARS-CoV-2), Iba1 (ionized calcium-binding adaptor molecule 1) and GFAP (glial fibrillary acidic protein) were quantified in retinal sections. The number of lectin vessels in COVID-19 retinas decreased by 27% compared to the control (p < 0.01) and the tortuosity increased in COVID-19 retinas (7.3 ± 0.2) vs. control retinas (6.8 ± 0.07) (p < 0.05). Immunofluorescence analysis revealed an increase in ACE2 (2.3 ± 1.3 vs. 1.0 ± 0.1; p < 0.0001) and Iba1 expression (3.06 ± 0.6 vs. 1.0 ± 0.1; p < 0.01) in COVID-19 sections whereas no changes in GFAP were observed. Analysis of the COVID-19 macular retinal tissue suggested that endothelial cells are a preferential target of SARS-CoV-2 with subsequent changes through their ACE2 receptor expression and morphology. Thus, microglial activation was hyperactive when facing an ensuing immunological challenge after SARS-CoV-2 infection. MDPI 2021-10-26 /pmc/articles/PMC8615149/ /pubmed/34829775 http://dx.doi.org/10.3390/biomedicines9111546 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hernandez, Maria González-Zamora, Jorge Recalde, Sergio Moreno-Orduña, Maite Bilbao-Malavé, Valentina Saenz de Viteri, Manuel Landecho, Manuel F. Fernandez-Robredo, Patricia García-Layana, Alfredo Evaluation of Macular Retinal Vessels and Histological Changes in Two Cases of COVID-19 |
title | Evaluation of Macular Retinal Vessels and Histological Changes in Two Cases of COVID-19 |
title_full | Evaluation of Macular Retinal Vessels and Histological Changes in Two Cases of COVID-19 |
title_fullStr | Evaluation of Macular Retinal Vessels and Histological Changes in Two Cases of COVID-19 |
title_full_unstemmed | Evaluation of Macular Retinal Vessels and Histological Changes in Two Cases of COVID-19 |
title_short | Evaluation of Macular Retinal Vessels and Histological Changes in Two Cases of COVID-19 |
title_sort | evaluation of macular retinal vessels and histological changes in two cases of covid-19 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615149/ https://www.ncbi.nlm.nih.gov/pubmed/34829775 http://dx.doi.org/10.3390/biomedicines9111546 |
work_keys_str_mv | AT hernandezmaria evaluationofmacularretinalvesselsandhistologicalchangesintwocasesofcovid19 AT gonzalezzamorajorge evaluationofmacularretinalvesselsandhistologicalchangesintwocasesofcovid19 AT recaldesergio evaluationofmacularretinalvesselsandhistologicalchangesintwocasesofcovid19 AT morenoordunamaite evaluationofmacularretinalvesselsandhistologicalchangesintwocasesofcovid19 AT bilbaomalavevalentina evaluationofmacularretinalvesselsandhistologicalchangesintwocasesofcovid19 AT saenzdeviterimanuel evaluationofmacularretinalvesselsandhistologicalchangesintwocasesofcovid19 AT landechomanuelf evaluationofmacularretinalvesselsandhistologicalchangesintwocasesofcovid19 AT fernandezrobredopatricia evaluationofmacularretinalvesselsandhistologicalchangesintwocasesofcovid19 AT garcialayanaalfredo evaluationofmacularretinalvesselsandhistologicalchangesintwocasesofcovid19 |