Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats

Oxidative stress plays a key role in the degeneration of dopaminergic neurons in Parkinson’s disease (PD), which may be aggravated by concomitant PD-associated gut dysbiosis. Probiotics and prebiotics are therapeutically relevant to these conditions due to their antioxidant, anti-inflammatory, and g...

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Autores principales: Tsao, Shu-Ping, Nurrahma, Bira Arumndari, Kumar, Ravi, Wu, Chieh-Hsi, Yeh, Tu-Hsueh, Chiu, Ching-Chi, Lee, Yen-Peng, Liao, Yi-Chi, Huang, Cheng-Hsieh, Yeh, Yao-Tsung, Huang, Hui-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615185/
https://www.ncbi.nlm.nih.gov/pubmed/34829694
http://dx.doi.org/10.3390/antiox10111823
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author Tsao, Shu-Ping
Nurrahma, Bira Arumndari
Kumar, Ravi
Wu, Chieh-Hsi
Yeh, Tu-Hsueh
Chiu, Ching-Chi
Lee, Yen-Peng
Liao, Yi-Chi
Huang, Cheng-Hsieh
Yeh, Yao-Tsung
Huang, Hui-Yu
author_facet Tsao, Shu-Ping
Nurrahma, Bira Arumndari
Kumar, Ravi
Wu, Chieh-Hsi
Yeh, Tu-Hsueh
Chiu, Ching-Chi
Lee, Yen-Peng
Liao, Yi-Chi
Huang, Cheng-Hsieh
Yeh, Yao-Tsung
Huang, Hui-Yu
author_sort Tsao, Shu-Ping
collection PubMed
description Oxidative stress plays a key role in the degeneration of dopaminergic neurons in Parkinson’s disease (PD), which may be aggravated by concomitant PD-associated gut dysbiosis. Probiotics and prebiotics are therapeutically relevant to these conditions due to their antioxidant, anti-inflammatory, and gut microbiome modulation properties. However, the mechanisms by which probiotic/prebiotic supplementation affects antioxidant capacity and the gut microbiome in PD remains poorly characterized. In this study, we assessed the effects of a Lactobacillus salivarius AP-32 probiotic, a prebiotic (dried AP-32 culture medium supernatant), and a probiotic/prebiotic cocktail in rats with unilateral 6-hydroxydopamine (6-OHDA)-induced PD. The neuroprotective effects and levels of oxidative stress were evaluated after eight weeks of daily supplementation. Fecal microbiota composition was analyzed by fecal 16S rRNA gene sequencing. The supplements were associated with direct increases in host antioxidant enzyme activities and short-chain fatty acid production, protected dopaminergic neurons, and improved motor functions. The supplements also altered the fecal microbiota composition, and some specifically enriched commensal taxa correlated positively with superoxide dismutase, glutathione peroxidase, and catalase activity, indicating supplementation also promotes antioxidant activity via an indirect pathway. Therefore, L. salivarius AP-32 supplementation enhanced the activity of host antioxidant enzymes via direct and indirect modes of action in rats with 6-OHDA-induced PD.
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spelling pubmed-86151852021-11-26 Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats Tsao, Shu-Ping Nurrahma, Bira Arumndari Kumar, Ravi Wu, Chieh-Hsi Yeh, Tu-Hsueh Chiu, Ching-Chi Lee, Yen-Peng Liao, Yi-Chi Huang, Cheng-Hsieh Yeh, Yao-Tsung Huang, Hui-Yu Antioxidants (Basel) Article Oxidative stress plays a key role in the degeneration of dopaminergic neurons in Parkinson’s disease (PD), which may be aggravated by concomitant PD-associated gut dysbiosis. Probiotics and prebiotics are therapeutically relevant to these conditions due to their antioxidant, anti-inflammatory, and gut microbiome modulation properties. However, the mechanisms by which probiotic/prebiotic supplementation affects antioxidant capacity and the gut microbiome in PD remains poorly characterized. In this study, we assessed the effects of a Lactobacillus salivarius AP-32 probiotic, a prebiotic (dried AP-32 culture medium supernatant), and a probiotic/prebiotic cocktail in rats with unilateral 6-hydroxydopamine (6-OHDA)-induced PD. The neuroprotective effects and levels of oxidative stress were evaluated after eight weeks of daily supplementation. Fecal microbiota composition was analyzed by fecal 16S rRNA gene sequencing. The supplements were associated with direct increases in host antioxidant enzyme activities and short-chain fatty acid production, protected dopaminergic neurons, and improved motor functions. The supplements also altered the fecal microbiota composition, and some specifically enriched commensal taxa correlated positively with superoxide dismutase, glutathione peroxidase, and catalase activity, indicating supplementation also promotes antioxidant activity via an indirect pathway. Therefore, L. salivarius AP-32 supplementation enhanced the activity of host antioxidant enzymes via direct and indirect modes of action in rats with 6-OHDA-induced PD. MDPI 2021-11-17 /pmc/articles/PMC8615185/ /pubmed/34829694 http://dx.doi.org/10.3390/antiox10111823 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsao, Shu-Ping
Nurrahma, Bira Arumndari
Kumar, Ravi
Wu, Chieh-Hsi
Yeh, Tu-Hsueh
Chiu, Ching-Chi
Lee, Yen-Peng
Liao, Yi-Chi
Huang, Cheng-Hsieh
Yeh, Yao-Tsung
Huang, Hui-Yu
Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_full Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_fullStr Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_full_unstemmed Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_short Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_sort probiotic enhancement of antioxidant capacity and alterations of gut microbiota composition in 6-hydroxydopamin-induced parkinson’s disease rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615185/
https://www.ncbi.nlm.nih.gov/pubmed/34829694
http://dx.doi.org/10.3390/antiox10111823
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