Cargando…

Direct Extraction of Fumaric Acid from Rhizopus oryzae Suspensions—Interfacial Mass Transfer

Experimental studies on the reactive extraction of fumaric acid with Amberlite LA-2 from Rhizopus oryzae suspensions using three solvents with different dielectric constants varying from 9.08 to 1.90 (dichloromethane, n-butyl acetate, and n-heptane, respectively) underlined the particular behavior o...

Descripción completa

Detalles Bibliográficos
Autores principales: Cascaval, Dan, Galaction, Anca-Irina, Tucaliuc, Alexandra, Kloetzer, Lenuta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615409/
https://www.ncbi.nlm.nih.gov/pubmed/34827561
http://dx.doi.org/10.3390/biom11111563
_version_ 1784604097650884608
author Cascaval, Dan
Galaction, Anca-Irina
Tucaliuc, Alexandra
Kloetzer, Lenuta
author_facet Cascaval, Dan
Galaction, Anca-Irina
Tucaliuc, Alexandra
Kloetzer, Lenuta
author_sort Cascaval, Dan
collection PubMed
description Experimental studies on the reactive extraction of fumaric acid with Amberlite LA-2 from Rhizopus oryzae suspensions using three solvents with different dielectric constants varying from 9.08 to 1.90 (dichloromethane, n-butyl acetate, and n-heptane, respectively) underlined the particular behavior of the extraction system in the presence of fungal biomass. The interfacial mass flow of the reaction product was found to be significantly affected by the biomass, due mainly to its adsorption onto the phase separation interface, this leading to the appearance of a physical barrier against the solute’s transfer. However, the magnitude of the adsorption phenomenon was found to depend on Rhizopus oryzae’s affinity for the solvent phase, which increased significantly from dichloromethane to n-heptane. The negative influence of the biomass on the interfacial mass transfer can be partially counteracted by adding 1-octanol into the organic phase, improving the solvent’s ability to solve the fumaric acid–Amberlite LA-2 complex and simplifying the reactive extraction mechanism, effects that were found to be more important for low-polar solvents. Consequently, for the same mixing intensity, the maximum amplification factor was reached for n-heptane, its value being almost 5–6 times higher than that obtained for dichloromethane and over 2 times higher than that obtained with n-butyl acetate.
format Online
Article
Text
id pubmed-8615409
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86154092021-11-26 Direct Extraction of Fumaric Acid from Rhizopus oryzae Suspensions—Interfacial Mass Transfer Cascaval, Dan Galaction, Anca-Irina Tucaliuc, Alexandra Kloetzer, Lenuta Biomolecules Article Experimental studies on the reactive extraction of fumaric acid with Amberlite LA-2 from Rhizopus oryzae suspensions using three solvents with different dielectric constants varying from 9.08 to 1.90 (dichloromethane, n-butyl acetate, and n-heptane, respectively) underlined the particular behavior of the extraction system in the presence of fungal biomass. The interfacial mass flow of the reaction product was found to be significantly affected by the biomass, due mainly to its adsorption onto the phase separation interface, this leading to the appearance of a physical barrier against the solute’s transfer. However, the magnitude of the adsorption phenomenon was found to depend on Rhizopus oryzae’s affinity for the solvent phase, which increased significantly from dichloromethane to n-heptane. The negative influence of the biomass on the interfacial mass transfer can be partially counteracted by adding 1-octanol into the organic phase, improving the solvent’s ability to solve the fumaric acid–Amberlite LA-2 complex and simplifying the reactive extraction mechanism, effects that were found to be more important for low-polar solvents. Consequently, for the same mixing intensity, the maximum amplification factor was reached for n-heptane, its value being almost 5–6 times higher than that obtained for dichloromethane and over 2 times higher than that obtained with n-butyl acetate. MDPI 2021-10-21 /pmc/articles/PMC8615409/ /pubmed/34827561 http://dx.doi.org/10.3390/biom11111563 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cascaval, Dan
Galaction, Anca-Irina
Tucaliuc, Alexandra
Kloetzer, Lenuta
Direct Extraction of Fumaric Acid from Rhizopus oryzae Suspensions—Interfacial Mass Transfer
title Direct Extraction of Fumaric Acid from Rhizopus oryzae Suspensions—Interfacial Mass Transfer
title_full Direct Extraction of Fumaric Acid from Rhizopus oryzae Suspensions—Interfacial Mass Transfer
title_fullStr Direct Extraction of Fumaric Acid from Rhizopus oryzae Suspensions—Interfacial Mass Transfer
title_full_unstemmed Direct Extraction of Fumaric Acid from Rhizopus oryzae Suspensions—Interfacial Mass Transfer
title_short Direct Extraction of Fumaric Acid from Rhizopus oryzae Suspensions—Interfacial Mass Transfer
title_sort direct extraction of fumaric acid from rhizopus oryzae suspensions—interfacial mass transfer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615409/
https://www.ncbi.nlm.nih.gov/pubmed/34827561
http://dx.doi.org/10.3390/biom11111563
work_keys_str_mv AT cascavaldan directextractionoffumaricacidfromrhizopusoryzaesuspensionsinterfacialmasstransfer
AT galactionancairina directextractionoffumaricacidfromrhizopusoryzaesuspensionsinterfacialmasstransfer
AT tucaliucalexandra directextractionoffumaricacidfromrhizopusoryzaesuspensionsinterfacialmasstransfer
AT kloetzerlenuta directextractionoffumaricacidfromrhizopusoryzaesuspensionsinterfacialmasstransfer