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An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates

The controlled release of antibiotics prevents the spread of pathogens and thereby improves healing processes in regenerative medicine. However, high concentrations may interfere with healing processes. It is therefore advantageous to use biodegradable materials for a controlled release. In particul...

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Autores principales: Kilb, Michelle Fiona, Moos, Yannik, Eckes, Stefanie, Braun, Joy, Ritz, Ulrike, Nickel, Daniela, Schmitz, Katja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615449/
https://www.ncbi.nlm.nih.gov/pubmed/34829897
http://dx.doi.org/10.3390/biomedicines9111668
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author Kilb, Michelle Fiona
Moos, Yannik
Eckes, Stefanie
Braun, Joy
Ritz, Ulrike
Nickel, Daniela
Schmitz, Katja
author_facet Kilb, Michelle Fiona
Moos, Yannik
Eckes, Stefanie
Braun, Joy
Ritz, Ulrike
Nickel, Daniela
Schmitz, Katja
author_sort Kilb, Michelle Fiona
collection PubMed
description The controlled release of antibiotics prevents the spread of pathogens and thereby improves healing processes in regenerative medicine. However, high concentrations may interfere with healing processes. It is therefore advantageous to use biodegradable materials for a controlled release. In particular, multilayer materials enable differential release at different surfaces. For this purpose, collagen sheets of different properties can be bonded by photochemical crosslinking. Here, we present the development and application of an easily accessible, additively manufactured sample holder to study the controlled release of vancomycin from modularly assembled collagen laminates in two directions. As proof-of-concept, we show that laminates of collagen sheets covalently linked by rose bengal and green light crosslinking (RGX) can be tightly inserted into the device without leakage from the upper to lower cavity. We used this sample holder to detect the release of vancomycin from symmetrically and asymmetrically loaded two-layer and three-layer collagen laminates into the upper and lower cavity of the sample holder. We show that these collagen laminates are characterized by a collagen type-dependent vancomycin release, enabling the control of antibiotic release profiles as well as the direction of antibiotic release.
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spelling pubmed-86154492021-11-26 An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates Kilb, Michelle Fiona Moos, Yannik Eckes, Stefanie Braun, Joy Ritz, Ulrike Nickel, Daniela Schmitz, Katja Biomedicines Article The controlled release of antibiotics prevents the spread of pathogens and thereby improves healing processes in regenerative medicine. However, high concentrations may interfere with healing processes. It is therefore advantageous to use biodegradable materials for a controlled release. In particular, multilayer materials enable differential release at different surfaces. For this purpose, collagen sheets of different properties can be bonded by photochemical crosslinking. Here, we present the development and application of an easily accessible, additively manufactured sample holder to study the controlled release of vancomycin from modularly assembled collagen laminates in two directions. As proof-of-concept, we show that laminates of collagen sheets covalently linked by rose bengal and green light crosslinking (RGX) can be tightly inserted into the device without leakage from the upper to lower cavity. We used this sample holder to detect the release of vancomycin from symmetrically and asymmetrically loaded two-layer and three-layer collagen laminates into the upper and lower cavity of the sample holder. We show that these collagen laminates are characterized by a collagen type-dependent vancomycin release, enabling the control of antibiotic release profiles as well as the direction of antibiotic release. MDPI 2021-11-11 /pmc/articles/PMC8615449/ /pubmed/34829897 http://dx.doi.org/10.3390/biomedicines9111668 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kilb, Michelle Fiona
Moos, Yannik
Eckes, Stefanie
Braun, Joy
Ritz, Ulrike
Nickel, Daniela
Schmitz, Katja
An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_full An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_fullStr An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_full_unstemmed An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_short An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_sort additively manufactured sample holder to measure the controlled release of vancomycin from collagen laminates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615449/
https://www.ncbi.nlm.nih.gov/pubmed/34829897
http://dx.doi.org/10.3390/biomedicines9111668
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