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Impact of Vitamin D(3) Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain

Vitamin D(3) hypovitaminosis is associated with several neurological diseases such as Alzheimer’s disease, Parkinson’s disease or multiple sclerosis but also with other diseases such as cancer, diabetes or diseases linked to inflammatory processes. Importantly, in all of these diseases lipids have a...

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Autores principales: Lauer, Anna Andrea, Griebsch, Lea Victoria, Pilz, Sabrina Melanie, Janitschke, Daniel, Theiss, Elena Leoni, Reichrath, Jörg, Herr, Christian, Beisswenger, Christoph, Bals, Robert, Valencak, Teresa Giovanna, Portius, Dorothea, Grimm, Heike Sabine, Hartmann, Tobias, Grimm, Marcus Otto Walter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615687/
https://www.ncbi.nlm.nih.gov/pubmed/34827697
http://dx.doi.org/10.3390/biom11111699
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author Lauer, Anna Andrea
Griebsch, Lea Victoria
Pilz, Sabrina Melanie
Janitschke, Daniel
Theiss, Elena Leoni
Reichrath, Jörg
Herr, Christian
Beisswenger, Christoph
Bals, Robert
Valencak, Teresa Giovanna
Portius, Dorothea
Grimm, Heike Sabine
Hartmann, Tobias
Grimm, Marcus Otto Walter
author_facet Lauer, Anna Andrea
Griebsch, Lea Victoria
Pilz, Sabrina Melanie
Janitschke, Daniel
Theiss, Elena Leoni
Reichrath, Jörg
Herr, Christian
Beisswenger, Christoph
Bals, Robert
Valencak, Teresa Giovanna
Portius, Dorothea
Grimm, Heike Sabine
Hartmann, Tobias
Grimm, Marcus Otto Walter
author_sort Lauer, Anna Andrea
collection PubMed
description Vitamin D(3) hypovitaminosis is associated with several neurological diseases such as Alzheimer’s disease, Parkinson’s disease or multiple sclerosis but also with other diseases such as cancer, diabetes or diseases linked to inflammatory processes. Importantly, in all of these diseases lipids have at least a disease modifying effect. Besides its well-known property to modulate gene-expression via the VDR-receptor, less is known if vitamin D hypovitaminosis influences lipid homeostasis and if these potential changes contribute to the pathology of the diseases themselves. Therefore, we analyzed mouse brain with a mild vitamin D hypovitaminosis via a targeted shotgun lipidomic approach, including phosphatidylcholine, plasmalogens, lyso-phosphatidylcholine, (acyl-/acetyl-) carnitines and triglycerides. Alterations were compared with neuroblastoma cells cultivated in the presence and with decreased levels of vitamin D. Both in cell culture and in vivo, decreased vitamin D level resulted in changed lipid levels. While triglycerides were decreased, carnitines were increased under vitamin D hypovitaminosis suggesting an impact of vitamin D on energy metabolism. Additionally, lyso-phosphatidylcholines in particular saturated phosphatidylcholine (e.g., PC aa 48:0) and plasmalogen species (e.g., PC ae 42:0) tended to be increased. Our results suggest that vitamin D hypovitaminosis not only may affect gene expression but also may directly influence cellular lipid homeostasis and affect lipid turnover in disease states that are known for vitamin D hypovitaminosis.
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spelling pubmed-86156872021-11-26 Impact of Vitamin D(3) Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain Lauer, Anna Andrea Griebsch, Lea Victoria Pilz, Sabrina Melanie Janitschke, Daniel Theiss, Elena Leoni Reichrath, Jörg Herr, Christian Beisswenger, Christoph Bals, Robert Valencak, Teresa Giovanna Portius, Dorothea Grimm, Heike Sabine Hartmann, Tobias Grimm, Marcus Otto Walter Biomolecules Article Vitamin D(3) hypovitaminosis is associated with several neurological diseases such as Alzheimer’s disease, Parkinson’s disease or multiple sclerosis but also with other diseases such as cancer, diabetes or diseases linked to inflammatory processes. Importantly, in all of these diseases lipids have at least a disease modifying effect. Besides its well-known property to modulate gene-expression via the VDR-receptor, less is known if vitamin D hypovitaminosis influences lipid homeostasis and if these potential changes contribute to the pathology of the diseases themselves. Therefore, we analyzed mouse brain with a mild vitamin D hypovitaminosis via a targeted shotgun lipidomic approach, including phosphatidylcholine, plasmalogens, lyso-phosphatidylcholine, (acyl-/acetyl-) carnitines and triglycerides. Alterations were compared with neuroblastoma cells cultivated in the presence and with decreased levels of vitamin D. Both in cell culture and in vivo, decreased vitamin D level resulted in changed lipid levels. While triglycerides were decreased, carnitines were increased under vitamin D hypovitaminosis suggesting an impact of vitamin D on energy metabolism. Additionally, lyso-phosphatidylcholines in particular saturated phosphatidylcholine (e.g., PC aa 48:0) and plasmalogen species (e.g., PC ae 42:0) tended to be increased. Our results suggest that vitamin D hypovitaminosis not only may affect gene expression but also may directly influence cellular lipid homeostasis and affect lipid turnover in disease states that are known for vitamin D hypovitaminosis. MDPI 2021-11-15 /pmc/articles/PMC8615687/ /pubmed/34827697 http://dx.doi.org/10.3390/biom11111699 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lauer, Anna Andrea
Griebsch, Lea Victoria
Pilz, Sabrina Melanie
Janitschke, Daniel
Theiss, Elena Leoni
Reichrath, Jörg
Herr, Christian
Beisswenger, Christoph
Bals, Robert
Valencak, Teresa Giovanna
Portius, Dorothea
Grimm, Heike Sabine
Hartmann, Tobias
Grimm, Marcus Otto Walter
Impact of Vitamin D(3) Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain
title Impact of Vitamin D(3) Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain
title_full Impact of Vitamin D(3) Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain
title_fullStr Impact of Vitamin D(3) Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain
title_full_unstemmed Impact of Vitamin D(3) Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain
title_short Impact of Vitamin D(3) Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain
title_sort impact of vitamin d(3) deficiency on phosphatidylcholine-/ethanolamine, plasmalogen-, lyso-phosphatidylcholine-/ethanolamine, carnitine- and triacyl glyceride-homeostasis in neuroblastoma cells and murine brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615687/
https://www.ncbi.nlm.nih.gov/pubmed/34827697
http://dx.doi.org/10.3390/biom11111699
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