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Microfluidic Raman Sensing Using a Single Ring Negative Curvature Hollow Core Fiber
A compact microfluidic Raman detection system based on a single-ring negative-curvature hollow-core fiber is presented. The system can be used for in-line qualitative and quantitative analysis of biochemicals. Both efficient light coupling and continuous liquid injection into the hollow-core fiber w...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615756/ https://www.ncbi.nlm.nih.gov/pubmed/34821646 http://dx.doi.org/10.3390/bios11110430 |
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author | Wang, Xinyu Li, Shuguang Gao, Shoufei Wang, Yingying Wang, Pu Ebendorff-Heidepriem, Heike Ruan, Yinlan |
author_facet | Wang, Xinyu Li, Shuguang Gao, Shoufei Wang, Yingying Wang, Pu Ebendorff-Heidepriem, Heike Ruan, Yinlan |
author_sort | Wang, Xinyu |
collection | PubMed |
description | A compact microfluidic Raman detection system based on a single-ring negative-curvature hollow-core fiber is presented. The system can be used for in-line qualitative and quantitative analysis of biochemicals. Both efficient light coupling and continuous liquid injection into the hollow-core fiber were achieved by creating a small gap between a solid-core fiber and the hollow-core fiber, which were fixed within a low-cost ceramic ferrule. A coupling efficiency of over 50% from free-space excitation laser to the hollow core fiber was obtained through a 350 μm-long solid-core fiber. For proof-of-concept demonstration of bioprocessing monitoring, a series of ethanol and glucose aqueous solutions at different concentrations were used. The limit of detection achieved for the ethanol solutions with our system was ~0.04 vol.% (0.32 g/L). Such an all-fiber microfluidic device is robust, provides Raman measurements with high repeatability and reusability, and is particularly suitable for the in-line monitoring of bioprocesses. |
format | Online Article Text |
id | pubmed-8615756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86157562021-11-26 Microfluidic Raman Sensing Using a Single Ring Negative Curvature Hollow Core Fiber Wang, Xinyu Li, Shuguang Gao, Shoufei Wang, Yingying Wang, Pu Ebendorff-Heidepriem, Heike Ruan, Yinlan Biosensors (Basel) Article A compact microfluidic Raman detection system based on a single-ring negative-curvature hollow-core fiber is presented. The system can be used for in-line qualitative and quantitative analysis of biochemicals. Both efficient light coupling and continuous liquid injection into the hollow-core fiber were achieved by creating a small gap between a solid-core fiber and the hollow-core fiber, which were fixed within a low-cost ceramic ferrule. A coupling efficiency of over 50% from free-space excitation laser to the hollow core fiber was obtained through a 350 μm-long solid-core fiber. For proof-of-concept demonstration of bioprocessing monitoring, a series of ethanol and glucose aqueous solutions at different concentrations were used. The limit of detection achieved for the ethanol solutions with our system was ~0.04 vol.% (0.32 g/L). Such an all-fiber microfluidic device is robust, provides Raman measurements with high repeatability and reusability, and is particularly suitable for the in-line monitoring of bioprocesses. MDPI 2021-10-30 /pmc/articles/PMC8615756/ /pubmed/34821646 http://dx.doi.org/10.3390/bios11110430 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Xinyu Li, Shuguang Gao, Shoufei Wang, Yingying Wang, Pu Ebendorff-Heidepriem, Heike Ruan, Yinlan Microfluidic Raman Sensing Using a Single Ring Negative Curvature Hollow Core Fiber |
title | Microfluidic Raman Sensing Using a Single Ring Negative Curvature Hollow Core Fiber |
title_full | Microfluidic Raman Sensing Using a Single Ring Negative Curvature Hollow Core Fiber |
title_fullStr | Microfluidic Raman Sensing Using a Single Ring Negative Curvature Hollow Core Fiber |
title_full_unstemmed | Microfluidic Raman Sensing Using a Single Ring Negative Curvature Hollow Core Fiber |
title_short | Microfluidic Raman Sensing Using a Single Ring Negative Curvature Hollow Core Fiber |
title_sort | microfluidic raman sensing using a single ring negative curvature hollow core fiber |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615756/ https://www.ncbi.nlm.nih.gov/pubmed/34821646 http://dx.doi.org/10.3390/bios11110430 |
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