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Targeting Brain Tumors with Mesenchymal Stem Cells in the Experimental Model of the Orthotopic Glioblastoma in Rats
Despite multimodal approaches for the treatment of multiforme glioblastoma (GBM) advances in outcome have been very modest indicating the necessity of novel diagnostic and therapeutic strategies. Currently, mesenchymal stem cells (MSCs) represent a promising platform for cell-based cancer therapies...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615766/ https://www.ncbi.nlm.nih.gov/pubmed/34829821 http://dx.doi.org/10.3390/biomedicines9111592 |
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author | Yudintceva, Natalia Lomert, Ekaterina Mikhailova, Natalia Tolkunova, Elena Agadzhanian, Nikol Samochernych, Konstantin Multhoff, Gabriele Timin, Grigoriy Ryzhov, Vyacheslav Deriglazov, Vladimir Mazur, Anton Shevtsov, Maxim |
author_facet | Yudintceva, Natalia Lomert, Ekaterina Mikhailova, Natalia Tolkunova, Elena Agadzhanian, Nikol Samochernych, Konstantin Multhoff, Gabriele Timin, Grigoriy Ryzhov, Vyacheslav Deriglazov, Vladimir Mazur, Anton Shevtsov, Maxim |
author_sort | Yudintceva, Natalia |
collection | PubMed |
description | Despite multimodal approaches for the treatment of multiforme glioblastoma (GBM) advances in outcome have been very modest indicating the necessity of novel diagnostic and therapeutic strategies. Currently, mesenchymal stem cells (MSCs) represent a promising platform for cell-based cancer therapies because of their tumor-tropism, low immunogenicity, easy accessibility, isolation procedure, and culturing. In the present study, we assessed the tumor-tropism and biodistribution of the superparamagnetic iron oxide nanoparticle (SPION)-labeled MSCs in the orthotopic model of C6 glioblastoma in Wistar rats. As shown in in vitro studies employing confocal microscopy, high-content quantitative image cytometer, and xCelligence system MSCs exhibit a high migratory capacity towards C6 glioblastoma cells. Intravenous administration of SPION-labeled MSCs in vivo resulted in intratumoral accumulation of the tagged cells in the tumor tissues that in turn significantly enhanced the contrast of the tumor when high-field magnetic resonance imaging was performed. Subsequent biodistribution studies employing highly sensitive nonlinear magnetic response measurements (NLR-M(2)) supported by histological analysis confirm the retention of MSCs in the glioblastoma. In conclusion, MSCs due to their tumor-tropism could be employed as a drug-delivery platform for future theranostic approaches. |
format | Online Article Text |
id | pubmed-8615766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86157662021-11-26 Targeting Brain Tumors with Mesenchymal Stem Cells in the Experimental Model of the Orthotopic Glioblastoma in Rats Yudintceva, Natalia Lomert, Ekaterina Mikhailova, Natalia Tolkunova, Elena Agadzhanian, Nikol Samochernych, Konstantin Multhoff, Gabriele Timin, Grigoriy Ryzhov, Vyacheslav Deriglazov, Vladimir Mazur, Anton Shevtsov, Maxim Biomedicines Article Despite multimodal approaches for the treatment of multiforme glioblastoma (GBM) advances in outcome have been very modest indicating the necessity of novel diagnostic and therapeutic strategies. Currently, mesenchymal stem cells (MSCs) represent a promising platform for cell-based cancer therapies because of their tumor-tropism, low immunogenicity, easy accessibility, isolation procedure, and culturing. In the present study, we assessed the tumor-tropism and biodistribution of the superparamagnetic iron oxide nanoparticle (SPION)-labeled MSCs in the orthotopic model of C6 glioblastoma in Wistar rats. As shown in in vitro studies employing confocal microscopy, high-content quantitative image cytometer, and xCelligence system MSCs exhibit a high migratory capacity towards C6 glioblastoma cells. Intravenous administration of SPION-labeled MSCs in vivo resulted in intratumoral accumulation of the tagged cells in the tumor tissues that in turn significantly enhanced the contrast of the tumor when high-field magnetic resonance imaging was performed. Subsequent biodistribution studies employing highly sensitive nonlinear magnetic response measurements (NLR-M(2)) supported by histological analysis confirm the retention of MSCs in the glioblastoma. In conclusion, MSCs due to their tumor-tropism could be employed as a drug-delivery platform for future theranostic approaches. MDPI 2021-11-01 /pmc/articles/PMC8615766/ /pubmed/34829821 http://dx.doi.org/10.3390/biomedicines9111592 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yudintceva, Natalia Lomert, Ekaterina Mikhailova, Natalia Tolkunova, Elena Agadzhanian, Nikol Samochernych, Konstantin Multhoff, Gabriele Timin, Grigoriy Ryzhov, Vyacheslav Deriglazov, Vladimir Mazur, Anton Shevtsov, Maxim Targeting Brain Tumors with Mesenchymal Stem Cells in the Experimental Model of the Orthotopic Glioblastoma in Rats |
title | Targeting Brain Tumors with Mesenchymal Stem Cells in the Experimental Model of the Orthotopic Glioblastoma in Rats |
title_full | Targeting Brain Tumors with Mesenchymal Stem Cells in the Experimental Model of the Orthotopic Glioblastoma in Rats |
title_fullStr | Targeting Brain Tumors with Mesenchymal Stem Cells in the Experimental Model of the Orthotopic Glioblastoma in Rats |
title_full_unstemmed | Targeting Brain Tumors with Mesenchymal Stem Cells in the Experimental Model of the Orthotopic Glioblastoma in Rats |
title_short | Targeting Brain Tumors with Mesenchymal Stem Cells in the Experimental Model of the Orthotopic Glioblastoma in Rats |
title_sort | targeting brain tumors with mesenchymal stem cells in the experimental model of the orthotopic glioblastoma in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615766/ https://www.ncbi.nlm.nih.gov/pubmed/34829821 http://dx.doi.org/10.3390/biomedicines9111592 |
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