Cargando…
Changes in the Expression of Renal Brush Border Membrane N-Glycome in Model Rats with Chronic Kidney Diseases
Chronic kidney disease (CKD) is defined by a reduced renal function i.e., glomerular filtration rate (GFR), and the presence of kidney damage is determined by measurement of proteinuria or albuminuria. Albuminuria increases with age and can result from glomerular and/or proximal tubule (PT) alterati...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616023/ https://www.ncbi.nlm.nih.gov/pubmed/34827675 http://dx.doi.org/10.3390/biom11111677 |
_version_ | 1784604247566843904 |
---|---|
author | Yu, Aiying Zhao, Jingfu Yadav, Shiv Pratap S. Molitoris, Bruce A. Wagner, Mark C. Mechref, Yehia |
author_facet | Yu, Aiying Zhao, Jingfu Yadav, Shiv Pratap S. Molitoris, Bruce A. Wagner, Mark C. Mechref, Yehia |
author_sort | Yu, Aiying |
collection | PubMed |
description | Chronic kidney disease (CKD) is defined by a reduced renal function i.e., glomerular filtration rate (GFR), and the presence of kidney damage is determined by measurement of proteinuria or albuminuria. Albuminuria increases with age and can result from glomerular and/or proximal tubule (PT) alterations. Brush-border membranes (BBMs) on PT cells play an important role in maintaining the stability of PT functions. The PT BBM, a highly dynamic, organized, specialized membrane, contains a variety of glycoproteins required for the functions of PT. Since protein glycosylation regulates many protein functions, the alteration of glycosylation due to the glycan changes has attracted more interests for a variety of disease studies recently. In this work, liquid chromatography-tandem mass spectrometry was utilized to analyze the abundances of permethylated glycans from rats under control to mild CKD, severe CKD, and diabetic conditions. The most significant differences were observed in sialylation level with the highest present in the severe CKD and diabetic groups. Moreover, high mannose N-glycans was enriched in the CKD BBMs. Characterization of all the BBM N-glycan changes supports that these changes are likely to impact the functional properties of the dynamic PT BBM. Further, these changes may lead to the potential discovery of glycan biomarkers for improved CKD diagnosis and new avenues for therapeutic treatments. |
format | Online Article Text |
id | pubmed-8616023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86160232021-11-26 Changes in the Expression of Renal Brush Border Membrane N-Glycome in Model Rats with Chronic Kidney Diseases Yu, Aiying Zhao, Jingfu Yadav, Shiv Pratap S. Molitoris, Bruce A. Wagner, Mark C. Mechref, Yehia Biomolecules Article Chronic kidney disease (CKD) is defined by a reduced renal function i.e., glomerular filtration rate (GFR), and the presence of kidney damage is determined by measurement of proteinuria or albuminuria. Albuminuria increases with age and can result from glomerular and/or proximal tubule (PT) alterations. Brush-border membranes (BBMs) on PT cells play an important role in maintaining the stability of PT functions. The PT BBM, a highly dynamic, organized, specialized membrane, contains a variety of glycoproteins required for the functions of PT. Since protein glycosylation regulates many protein functions, the alteration of glycosylation due to the glycan changes has attracted more interests for a variety of disease studies recently. In this work, liquid chromatography-tandem mass spectrometry was utilized to analyze the abundances of permethylated glycans from rats under control to mild CKD, severe CKD, and diabetic conditions. The most significant differences were observed in sialylation level with the highest present in the severe CKD and diabetic groups. Moreover, high mannose N-glycans was enriched in the CKD BBMs. Characterization of all the BBM N-glycan changes supports that these changes are likely to impact the functional properties of the dynamic PT BBM. Further, these changes may lead to the potential discovery of glycan biomarkers for improved CKD diagnosis and new avenues for therapeutic treatments. MDPI 2021-11-11 /pmc/articles/PMC8616023/ /pubmed/34827675 http://dx.doi.org/10.3390/biom11111677 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yu, Aiying Zhao, Jingfu Yadav, Shiv Pratap S. Molitoris, Bruce A. Wagner, Mark C. Mechref, Yehia Changes in the Expression of Renal Brush Border Membrane N-Glycome in Model Rats with Chronic Kidney Diseases |
title | Changes in the Expression of Renal Brush Border Membrane N-Glycome in Model Rats with Chronic Kidney Diseases |
title_full | Changes in the Expression of Renal Brush Border Membrane N-Glycome in Model Rats with Chronic Kidney Diseases |
title_fullStr | Changes in the Expression of Renal Brush Border Membrane N-Glycome in Model Rats with Chronic Kidney Diseases |
title_full_unstemmed | Changes in the Expression of Renal Brush Border Membrane N-Glycome in Model Rats with Chronic Kidney Diseases |
title_short | Changes in the Expression of Renal Brush Border Membrane N-Glycome in Model Rats with Chronic Kidney Diseases |
title_sort | changes in the expression of renal brush border membrane n-glycome in model rats with chronic kidney diseases |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616023/ https://www.ncbi.nlm.nih.gov/pubmed/34827675 http://dx.doi.org/10.3390/biom11111677 |
work_keys_str_mv | AT yuaiying changesintheexpressionofrenalbrushbordermembranenglycomeinmodelratswithchronickidneydiseases AT zhaojingfu changesintheexpressionofrenalbrushbordermembranenglycomeinmodelratswithchronickidneydiseases AT yadavshivprataps changesintheexpressionofrenalbrushbordermembranenglycomeinmodelratswithchronickidneydiseases AT molitorisbrucea changesintheexpressionofrenalbrushbordermembranenglycomeinmodelratswithchronickidneydiseases AT wagnermarkc changesintheexpressionofrenalbrushbordermembranenglycomeinmodelratswithchronickidneydiseases AT mechrefyehia changesintheexpressionofrenalbrushbordermembranenglycomeinmodelratswithchronickidneydiseases |