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A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion

Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chi...

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Autores principales: Pendharkar, Gaurav, Lu, Yen-Ta, Chang, Chia-Ming, Lu, Meng-Ping, Lu, Chung-Huan, Chen, Chih-Chen, Liu, Cheng-Hsien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616069/
https://www.ncbi.nlm.nih.gov/pubmed/34831078
http://dx.doi.org/10.3390/cells10112855
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author Pendharkar, Gaurav
Lu, Yen-Ta
Chang, Chia-Ming
Lu, Meng-Ping
Lu, Chung-Huan
Chen, Chih-Chen
Liu, Cheng-Hsien
author_facet Pendharkar, Gaurav
Lu, Yen-Ta
Chang, Chia-Ming
Lu, Meng-Ping
Lu, Chung-Huan
Chen, Chih-Chen
Liu, Cheng-Hsien
author_sort Pendharkar, Gaurav
collection PubMed
description Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis.
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spelling pubmed-86160692021-11-26 A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion Pendharkar, Gaurav Lu, Yen-Ta Chang, Chia-Ming Lu, Meng-Ping Lu, Chung-Huan Chen, Chih-Chen Liu, Cheng-Hsien Cells Article Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis. MDPI 2021-10-22 /pmc/articles/PMC8616069/ /pubmed/34831078 http://dx.doi.org/10.3390/cells10112855 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pendharkar, Gaurav
Lu, Yen-Ta
Chang, Chia-Ming
Lu, Meng-Ping
Lu, Chung-Huan
Chen, Chih-Chen
Liu, Cheng-Hsien
A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_full A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_fullStr A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_full_unstemmed A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_short A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
title_sort microfluidic flip-chip combining hydrodynamic trapping and gravitational sedimentation for cell pairing and fusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616069/
https://www.ncbi.nlm.nih.gov/pubmed/34831078
http://dx.doi.org/10.3390/cells10112855
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