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A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues

Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may release products that induce apoptosis of host cel...

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Autores principales: Saiki, Haruko, Okano, Yuko, Yasuma, Taro, Toda, Masaaki, Takeshita, Atsuro, Abdel-Hamid, Ahmed M., Fridman D’Alessandro, Valeria, Tsuruga, Tatsuki, D’Alessandro-Gabazza, Corina N., Katayama, Kan, Sugimoto, Masahiko, Fujimoto, Hajime, Yamanaka, Keiichi, Kobayashi, Tetsu, Cann, Isaac, Gabazza, Esteban C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616533/
https://www.ncbi.nlm.nih.gov/pubmed/34831108
http://dx.doi.org/10.3390/cells10112885
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author Saiki, Haruko
Okano, Yuko
Yasuma, Taro
Toda, Masaaki
Takeshita, Atsuro
Abdel-Hamid, Ahmed M.
Fridman D’Alessandro, Valeria
Tsuruga, Tatsuki
D’Alessandro-Gabazza, Corina N.
Katayama, Kan
Sugimoto, Masahiko
Fujimoto, Hajime
Yamanaka, Keiichi
Kobayashi, Tetsu
Cann, Isaac
Gabazza, Esteban C.
author_facet Saiki, Haruko
Okano, Yuko
Yasuma, Taro
Toda, Masaaki
Takeshita, Atsuro
Abdel-Hamid, Ahmed M.
Fridman D’Alessandro, Valeria
Tsuruga, Tatsuki
D’Alessandro-Gabazza, Corina N.
Katayama, Kan
Sugimoto, Masahiko
Fujimoto, Hajime
Yamanaka, Keiichi
Kobayashi, Tetsu
Cann, Isaac
Gabazza, Esteban C.
author_sort Saiki, Haruko
collection PubMed
description Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may release products that induce apoptosis of host cells. We recently identified a novel microbiome-derived peptide called corisin that worsens lung fibrosis by inducing apoptosis of lung epithelial cells. We hypothesized that corisin and a corisin-like peptide might also induce apoptosis of cells from different tissues. We cultured podocytes, renal tubular epithelial cells, keratinocytes, retinal and intestinal cells treated with corisin and evaluated apoptosis by flow cytometry and Western blotting. Although at different grades, flow cytometry analysis and Western blotting showed that corisin and a corisin-like peptide induced apoptosis of podocytes, keratinocytes, tubular epithelial cells, retinal, and intestinal cells. In addition, we found that corisin synergistically enhances the proapoptotic activity of transforming growth factor-β1 on podocytes. In conclusion, these results suggest that corisin and corisin-like peptides may play a role in the pathogenesis of disease in different organs by promoting apoptosis of parenchymal cells.
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spelling pubmed-86165332021-11-26 A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues Saiki, Haruko Okano, Yuko Yasuma, Taro Toda, Masaaki Takeshita, Atsuro Abdel-Hamid, Ahmed M. Fridman D’Alessandro, Valeria Tsuruga, Tatsuki D’Alessandro-Gabazza, Corina N. Katayama, Kan Sugimoto, Masahiko Fujimoto, Hajime Yamanaka, Keiichi Kobayashi, Tetsu Cann, Isaac Gabazza, Esteban C. Cells Communication Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may release products that induce apoptosis of host cells. We recently identified a novel microbiome-derived peptide called corisin that worsens lung fibrosis by inducing apoptosis of lung epithelial cells. We hypothesized that corisin and a corisin-like peptide might also induce apoptosis of cells from different tissues. We cultured podocytes, renal tubular epithelial cells, keratinocytes, retinal and intestinal cells treated with corisin and evaluated apoptosis by flow cytometry and Western blotting. Although at different grades, flow cytometry analysis and Western blotting showed that corisin and a corisin-like peptide induced apoptosis of podocytes, keratinocytes, tubular epithelial cells, retinal, and intestinal cells. In addition, we found that corisin synergistically enhances the proapoptotic activity of transforming growth factor-β1 on podocytes. In conclusion, these results suggest that corisin and corisin-like peptides may play a role in the pathogenesis of disease in different organs by promoting apoptosis of parenchymal cells. MDPI 2021-10-26 /pmc/articles/PMC8616533/ /pubmed/34831108 http://dx.doi.org/10.3390/cells10112885 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Saiki, Haruko
Okano, Yuko
Yasuma, Taro
Toda, Masaaki
Takeshita, Atsuro
Abdel-Hamid, Ahmed M.
Fridman D’Alessandro, Valeria
Tsuruga, Tatsuki
D’Alessandro-Gabazza, Corina N.
Katayama, Kan
Sugimoto, Masahiko
Fujimoto, Hajime
Yamanaka, Keiichi
Kobayashi, Tetsu
Cann, Isaac
Gabazza, Esteban C.
A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_full A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_fullStr A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_full_unstemmed A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_short A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues
title_sort microbiome-derived peptide induces apoptosis of cells from different tissues
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616533/
https://www.ncbi.nlm.nih.gov/pubmed/34831108
http://dx.doi.org/10.3390/cells10112885
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