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Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases
Protein glycosylation in the Golgi is a sequential process that requires proper distribution of transmembrane glycosyltransferase enzymes in the appropriate Golgi compartments. Some of the cytosolic machinery required for the steady-state localization of some Golgi enzymes are known but existing mod...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616560/ https://www.ncbi.nlm.nih.gov/pubmed/34821548 http://dx.doi.org/10.7554/eLife.70774 |
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author | Sardana, Richa Highland, Carolyn M Straight, Beth E Chavez, Christopher F Fromme, J Christopher Emr, Scott D |
author_facet | Sardana, Richa Highland, Carolyn M Straight, Beth E Chavez, Christopher F Fromme, J Christopher Emr, Scott D |
author_sort | Sardana, Richa |
collection | PubMed |
description | Protein glycosylation in the Golgi is a sequential process that requires proper distribution of transmembrane glycosyltransferase enzymes in the appropriate Golgi compartments. Some of the cytosolic machinery required for the steady-state localization of some Golgi enzymes are known but existing models do not explain how many of these enzymes are localized. Here, we uncover the role of an integral membrane protein in yeast, Erd1, as a key facilitator of Golgi glycosyltransferase recycling by directly interacting with both the Golgi enzymes and the cytosolic receptor, Vps74. Loss of Erd1 function results in mislocalization of Golgi enzymes to the vacuole/lysosome. We present evidence that Erd1 forms an integral part of the recycling machinery and ensures productive recycling of several early Golgi enzymes. Our work provides new insights on how the localization of Golgi glycosyltransferases is spatially and temporally regulated, and is finely tuned to the cues of Golgi maturation. |
format | Online Article Text |
id | pubmed-8616560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-86165602021-11-26 Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases Sardana, Richa Highland, Carolyn M Straight, Beth E Chavez, Christopher F Fromme, J Christopher Emr, Scott D eLife Cell Biology Protein glycosylation in the Golgi is a sequential process that requires proper distribution of transmembrane glycosyltransferase enzymes in the appropriate Golgi compartments. Some of the cytosolic machinery required for the steady-state localization of some Golgi enzymes are known but existing models do not explain how many of these enzymes are localized. Here, we uncover the role of an integral membrane protein in yeast, Erd1, as a key facilitator of Golgi glycosyltransferase recycling by directly interacting with both the Golgi enzymes and the cytosolic receptor, Vps74. Loss of Erd1 function results in mislocalization of Golgi enzymes to the vacuole/lysosome. We present evidence that Erd1 forms an integral part of the recycling machinery and ensures productive recycling of several early Golgi enzymes. Our work provides new insights on how the localization of Golgi glycosyltransferases is spatially and temporally regulated, and is finely tuned to the cues of Golgi maturation. eLife Sciences Publications, Ltd 2021-11-25 /pmc/articles/PMC8616560/ /pubmed/34821548 http://dx.doi.org/10.7554/eLife.70774 Text en © 2021, Sardana et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Sardana, Richa Highland, Carolyn M Straight, Beth E Chavez, Christopher F Fromme, J Christopher Emr, Scott D Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases |
title | Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases |
title_full | Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases |
title_fullStr | Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases |
title_full_unstemmed | Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases |
title_short | Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases |
title_sort | golgi membrane protein erd1 is essential for recycling a subset of golgi glycosyltransferases |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616560/ https://www.ncbi.nlm.nih.gov/pubmed/34821548 http://dx.doi.org/10.7554/eLife.70774 |
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