Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS)

Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner) transpo...

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Detalles Bibliográficos
Autores principales: Bossé, Janine T., Li, Yanwen, Leanse, Leon G., Zhou, Liqing, Chaudhuri, Roy R., Peters, Sarah E., Wang, Jinhong, Maglennon, Gareth A., Holden, Matthew T. G., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., Langford, Paul R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616859/
https://www.ncbi.nlm.nih.gov/pubmed/34870287
http://dx.doi.org/10.1186/s44149-021-00026-4

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