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Planar photonic chips with tailored angular transmission for high-contrast-imaging devices

A limitation of standard brightfield microscopy is its low contrast images, especially for thin specimens of weak absorption, and biological species with refractive indices very close in value to that of their surroundings. We demonstrate, using a planar photonic chip with tailored angular transmiss...

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Detalles Bibliográficos
Autores principales: Kuai, Yan, Chen, Junxue, Fan, Zetao, Zou, Gang, Lakowicz, Joseph. R., Zhang, Douguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616932/
https://www.ncbi.nlm.nih.gov/pubmed/34824261
http://dx.doi.org/10.1038/s41467-021-27231-6
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author Kuai, Yan
Chen, Junxue
Fan, Zetao
Zou, Gang
Lakowicz, Joseph. R.
Zhang, Douguo
author_facet Kuai, Yan
Chen, Junxue
Fan, Zetao
Zou, Gang
Lakowicz, Joseph. R.
Zhang, Douguo
author_sort Kuai, Yan
collection PubMed
description A limitation of standard brightfield microscopy is its low contrast images, especially for thin specimens of weak absorption, and biological species with refractive indices very close in value to that of their surroundings. We demonstrate, using a planar photonic chip with tailored angular transmission as the sample substrate, a standard brightfield microscopy can provide both darkfield and total internal reflection (TIR) microscopy images with one experimental configuration. The image contrast is enhanced without altering the specimens and the microscope configurations. This planar chip consists of several multilayer sections with designed photonic band gaps and a central region with dielectric nanoparticles, which does not require top-down nanofabrication and can be fabricated in a larger scale. The photonic chip eliminates the need for a bulky condenser or special objective to realize darkfield or TIR illumination. Thus, it can work as a miniaturized high-contrast-imaging device for the developments of versatile and compact microscopes.
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spelling pubmed-86169322021-12-01 Planar photonic chips with tailored angular transmission for high-contrast-imaging devices Kuai, Yan Chen, Junxue Fan, Zetao Zou, Gang Lakowicz, Joseph. R. Zhang, Douguo Nat Commun Article A limitation of standard brightfield microscopy is its low contrast images, especially for thin specimens of weak absorption, and biological species with refractive indices very close in value to that of their surroundings. We demonstrate, using a planar photonic chip with tailored angular transmission as the sample substrate, a standard brightfield microscopy can provide both darkfield and total internal reflection (TIR) microscopy images with one experimental configuration. The image contrast is enhanced without altering the specimens and the microscope configurations. This planar chip consists of several multilayer sections with designed photonic band gaps and a central region with dielectric nanoparticles, which does not require top-down nanofabrication and can be fabricated in a larger scale. The photonic chip eliminates the need for a bulky condenser or special objective to realize darkfield or TIR illumination. Thus, it can work as a miniaturized high-contrast-imaging device for the developments of versatile and compact microscopes. Nature Publishing Group UK 2021-11-25 /pmc/articles/PMC8616932/ /pubmed/34824261 http://dx.doi.org/10.1038/s41467-021-27231-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kuai, Yan
Chen, Junxue
Fan, Zetao
Zou, Gang
Lakowicz, Joseph. R.
Zhang, Douguo
Planar photonic chips with tailored angular transmission for high-contrast-imaging devices
title Planar photonic chips with tailored angular transmission for high-contrast-imaging devices
title_full Planar photonic chips with tailored angular transmission for high-contrast-imaging devices
title_fullStr Planar photonic chips with tailored angular transmission for high-contrast-imaging devices
title_full_unstemmed Planar photonic chips with tailored angular transmission for high-contrast-imaging devices
title_short Planar photonic chips with tailored angular transmission for high-contrast-imaging devices
title_sort planar photonic chips with tailored angular transmission for high-contrast-imaging devices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616932/
https://www.ncbi.nlm.nih.gov/pubmed/34824261
http://dx.doi.org/10.1038/s41467-021-27231-6
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