Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor

The RNA-guided CRISPR-associated Cas9 proteins have been widely applied in programmable genome recombination, base editing or gene regulation in both prokaryotes and eukaryotes. SpCas9 from Streptococcus pyogenes is the most extensively engineered Cas9 with robust and manifold functionalities. Howev...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jian, Teng, Yuxi, Zhang, Ruihua, Wu, Yifei, Lou, Lei, Zou, Yusong, Li, Michelle, Xie, Zhong-Ru, Yan, Yajun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617050/
https://www.ncbi.nlm.nih.gov/pubmed/34824292
http://dx.doi.org/10.1038/s41467-021-27290-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617050/
https://www.ncbi.nlm.nih.gov/pubmed/34824292
http://dx.doi.org/10.1038/s41467-021-27290-9

Ejemplares similares