Cargando…

Cell-fate transition and determination analysis of mouse male germ cells throughout development

Mammalian male germ cell development is a stepwise cell-fate transition process; however, the full-term developmental profile of male germ cells remains undefined. Here, by interrogating the high-precision transcriptome atlas of 11,598 cells covering 28 critical time-points, we demonstrate that cell...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Jiexiang, Lu, Ping, Wan, Cong, Huang, Yaping, Cui, Manman, Yang, Xinyan, Hu, Yuqiong, Zheng, Yi, Dong, Ji, Wang, Mei, Zhang, Shu, Liu, Zhaoting, Bian, Shuhui, Wang, Xiaoman, Wang, Rui, Ren, Shaofang, Wang, Dazhuang, Yao, Zhaokai, Chang, Gang, Tang, Fuchou, Zhao, Xiao-Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617176/
https://www.ncbi.nlm.nih.gov/pubmed/34824237
http://dx.doi.org/10.1038/s41467-021-27172-0
_version_ 1784604474780680192
author Zhao, Jiexiang
Lu, Ping
Wan, Cong
Huang, Yaping
Cui, Manman
Yang, Xinyan
Hu, Yuqiong
Zheng, Yi
Dong, Ji
Wang, Mei
Zhang, Shu
Liu, Zhaoting
Bian, Shuhui
Wang, Xiaoman
Wang, Rui
Ren, Shaofang
Wang, Dazhuang
Yao, Zhaokai
Chang, Gang
Tang, Fuchou
Zhao, Xiao-Yang
author_facet Zhao, Jiexiang
Lu, Ping
Wan, Cong
Huang, Yaping
Cui, Manman
Yang, Xinyan
Hu, Yuqiong
Zheng, Yi
Dong, Ji
Wang, Mei
Zhang, Shu
Liu, Zhaoting
Bian, Shuhui
Wang, Xiaoman
Wang, Rui
Ren, Shaofang
Wang, Dazhuang
Yao, Zhaokai
Chang, Gang
Tang, Fuchou
Zhao, Xiao-Yang
author_sort Zhao, Jiexiang
collection PubMed
description Mammalian male germ cell development is a stepwise cell-fate transition process; however, the full-term developmental profile of male germ cells remains undefined. Here, by interrogating the high-precision transcriptome atlas of 11,598 cells covering 28 critical time-points, we demonstrate that cell-fate transition from mitotic to post-mitotic primordial germ cells is accompanied by transcriptome-scale reconfiguration and a transitional cell state. Notch signaling pathway is essential for initiating mitotic arrest and the maintenance of male germ cells’ identities. Ablation of HELQ induces developmental arrest and abnormal transcriptome reprogramming of male germ cells, indicating the importance of cell cycle regulation for proper cell-fate transition. Finally, systematic human-mouse comparison reveals potential regulators whose deficiency contributed to human male infertility via mitotic arrest regulation. Collectively, our study provides an accurate and comprehensive transcriptome atlas of the male germline cycle and allows for an in-depth understanding of the cell-fate transition and determination underlying male germ cell development.
format Online
Article
Text
id pubmed-8617176
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-86171762021-12-10 Cell-fate transition and determination analysis of mouse male germ cells throughout development Zhao, Jiexiang Lu, Ping Wan, Cong Huang, Yaping Cui, Manman Yang, Xinyan Hu, Yuqiong Zheng, Yi Dong, Ji Wang, Mei Zhang, Shu Liu, Zhaoting Bian, Shuhui Wang, Xiaoman Wang, Rui Ren, Shaofang Wang, Dazhuang Yao, Zhaokai Chang, Gang Tang, Fuchou Zhao, Xiao-Yang Nat Commun Article Mammalian male germ cell development is a stepwise cell-fate transition process; however, the full-term developmental profile of male germ cells remains undefined. Here, by interrogating the high-precision transcriptome atlas of 11,598 cells covering 28 critical time-points, we demonstrate that cell-fate transition from mitotic to post-mitotic primordial germ cells is accompanied by transcriptome-scale reconfiguration and a transitional cell state. Notch signaling pathway is essential for initiating mitotic arrest and the maintenance of male germ cells’ identities. Ablation of HELQ induces developmental arrest and abnormal transcriptome reprogramming of male germ cells, indicating the importance of cell cycle regulation for proper cell-fate transition. Finally, systematic human-mouse comparison reveals potential regulators whose deficiency contributed to human male infertility via mitotic arrest regulation. Collectively, our study provides an accurate and comprehensive transcriptome atlas of the male germline cycle and allows for an in-depth understanding of the cell-fate transition and determination underlying male germ cell development. Nature Publishing Group UK 2021-11-25 /pmc/articles/PMC8617176/ /pubmed/34824237 http://dx.doi.org/10.1038/s41467-021-27172-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhao, Jiexiang
Lu, Ping
Wan, Cong
Huang, Yaping
Cui, Manman
Yang, Xinyan
Hu, Yuqiong
Zheng, Yi
Dong, Ji
Wang, Mei
Zhang, Shu
Liu, Zhaoting
Bian, Shuhui
Wang, Xiaoman
Wang, Rui
Ren, Shaofang
Wang, Dazhuang
Yao, Zhaokai
Chang, Gang
Tang, Fuchou
Zhao, Xiao-Yang
Cell-fate transition and determination analysis of mouse male germ cells throughout development
title Cell-fate transition and determination analysis of mouse male germ cells throughout development
title_full Cell-fate transition and determination analysis of mouse male germ cells throughout development
title_fullStr Cell-fate transition and determination analysis of mouse male germ cells throughout development
title_full_unstemmed Cell-fate transition and determination analysis of mouse male germ cells throughout development
title_short Cell-fate transition and determination analysis of mouse male germ cells throughout development
title_sort cell-fate transition and determination analysis of mouse male germ cells throughout development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617176/
https://www.ncbi.nlm.nih.gov/pubmed/34824237
http://dx.doi.org/10.1038/s41467-021-27172-0
work_keys_str_mv AT zhaojiexiang cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT luping cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT wancong cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT huangyaping cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT cuimanman cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT yangxinyan cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT huyuqiong cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT zhengyi cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT dongji cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT wangmei cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT zhangshu cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT liuzhaoting cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT bianshuhui cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT wangxiaoman cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT wangrui cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT renshaofang cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT wangdazhuang cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT yaozhaokai cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT changgang cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT tangfuchou cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment
AT zhaoxiaoyang cellfatetransitionanddeterminationanalysisofmousemalegermcellsthroughoutdevelopment