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Three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of SARS-CoV-2 particles
SARS-CoV-2 is the cause of COVID-19. The three-dimensional morphology of viral particles existing and multiplying in infected cells has not been established by electron tomography, which is different from cryo-electron tomography using frozen samples. In this study, we establish the morphological st...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617558/ https://www.ncbi.nlm.nih.gov/pubmed/34825978 http://dx.doi.org/10.1007/s00795-021-00309-2 |
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author | Wu, Hong Fujioka, Yoshihiko Sakaguchi, Shoichi Suzuki, Youichi Nakano, Takashi |
author_facet | Wu, Hong Fujioka, Yoshihiko Sakaguchi, Shoichi Suzuki, Youichi Nakano, Takashi |
author_sort | Wu, Hong |
collection | PubMed |
description | SARS-CoV-2 is the cause of COVID-19. The three-dimensional morphology of viral particles existing and multiplying in infected cells has not been established by electron tomography, which is different from cryo-electron tomography using frozen samples. In this study, we establish the morphological structure of SARS-CoV-2 particles by three-dimensional reconstruction of images obtained by electron tomography and transmission electron microscopy of biological samples embedded in epoxy resin. The characteristic roots of spike structures were found to be arranged at the surface of a virion covered with an envelope. A high-electron-density structure that appears to be a nucleocapsid was observed inside the envelope of the virion on three-dimensional images reconstructed by electron tomography. The SARS-CoV-2 particles that budded in the vacuoles in the cytoplasm were morphologically identical to those found outside the cells, suggesting that mature and infectious SARS-CoV-2 particles were already produced in the vacuoles. Here, we show the three-dimensional morphological structure of SARS-CoV-2 particles reconstructed by electron tomography. To control infection, inhibition of viral release from vacuoles would be a new target in the development of prophylactic agents against SARS-CoV-2. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00795-021-00309-2. |
format | Online Article Text |
id | pubmed-8617558 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-86175582021-11-26 Three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of SARS-CoV-2 particles Wu, Hong Fujioka, Yoshihiko Sakaguchi, Shoichi Suzuki, Youichi Nakano, Takashi Med Mol Morphol Original Paper SARS-CoV-2 is the cause of COVID-19. The three-dimensional morphology of viral particles existing and multiplying in infected cells has not been established by electron tomography, which is different from cryo-electron tomography using frozen samples. In this study, we establish the morphological structure of SARS-CoV-2 particles by three-dimensional reconstruction of images obtained by electron tomography and transmission electron microscopy of biological samples embedded in epoxy resin. The characteristic roots of spike structures were found to be arranged at the surface of a virion covered with an envelope. A high-electron-density structure that appears to be a nucleocapsid was observed inside the envelope of the virion on three-dimensional images reconstructed by electron tomography. The SARS-CoV-2 particles that budded in the vacuoles in the cytoplasm were morphologically identical to those found outside the cells, suggesting that mature and infectious SARS-CoV-2 particles were already produced in the vacuoles. Here, we show the three-dimensional morphological structure of SARS-CoV-2 particles reconstructed by electron tomography. To control infection, inhibition of viral release from vacuoles would be a new target in the development of prophylactic agents against SARS-CoV-2. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00795-021-00309-2. Springer Singapore 2021-11-26 2022 /pmc/articles/PMC8617558/ /pubmed/34825978 http://dx.doi.org/10.1007/s00795-021-00309-2 Text en © The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Paper Wu, Hong Fujioka, Yoshihiko Sakaguchi, Shoichi Suzuki, Youichi Nakano, Takashi Three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of SARS-CoV-2 particles |
title | Three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of SARS-CoV-2 particles |
title_full | Three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of SARS-CoV-2 particles |
title_fullStr | Three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of SARS-CoV-2 particles |
title_full_unstemmed | Three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of SARS-CoV-2 particles |
title_short | Three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of SARS-CoV-2 particles |
title_sort | three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of sars-cov-2 particles |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617558/ https://www.ncbi.nlm.nih.gov/pubmed/34825978 http://dx.doi.org/10.1007/s00795-021-00309-2 |
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