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Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice

We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of Trichinella spiralis (T. spiralis) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of the MSUS/MEX/91/CM-91 T. spiralis strain...

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Autores principales: Vega-Sánchez, Vicente, Gómez-De-Anda, Fabián-Ricardo, Calderón-Domínguez, Georgina, Ramírez-y-Ramírez, Mary-Carmen-del-Sol, Reyes-Rodríguez, Nydia-E., Zepeda-Velázquez, Andrea-P., Tapia-Romero, Raquel, de-la-Rosa-Arana, Jorge-Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617616/
https://www.ncbi.nlm.nih.gov/pubmed/34832538
http://dx.doi.org/10.3390/pathogens10111382
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author Vega-Sánchez, Vicente
Gómez-De-Anda, Fabián-Ricardo
Calderón-Domínguez, Georgina
Ramírez-y-Ramírez, Mary-Carmen-del-Sol
Reyes-Rodríguez, Nydia-E.
Zepeda-Velázquez, Andrea-P.
Tapia-Romero, Raquel
de-la-Rosa-Arana, Jorge-Luis
author_facet Vega-Sánchez, Vicente
Gómez-De-Anda, Fabián-Ricardo
Calderón-Domínguez, Georgina
Ramírez-y-Ramírez, Mary-Carmen-del-Sol
Reyes-Rodríguez, Nydia-E.
Zepeda-Velázquez, Andrea-P.
Tapia-Romero, Raquel
de-la-Rosa-Arana, Jorge-Luis
author_sort Vega-Sánchez, Vicente
collection PubMed
description We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of Trichinella spiralis (T. spiralis) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of the MSUS/MEX/91/CM-91 T. spiralis strain. Tongues and diaphragms were obtained daily from days 13 to 39 post infection. Diaphragms were compressed and subjected to Giemsa stain. Tongues were histologically sectioned and stained with erythrosine B or hematoxylin and eosin. The cellular infiltrate and the nurse cell-larva complex were detected by optical microscopy since day 16 post infection. The size of the larva increased exponentially during the course of the infection. The kinetics of eosinophils showed a multimodal trend, with a bimodal predominance. The maximum peaks were reached on days 21 and 27 post infection. The results of this study demonstrate that eosinophils occur abundantly in two transcendent moments of the T. spiralis life cycle: first, when the stage 1 larva invades the myocyte and second when the nurse cell-larva complex has been fully developed. These results help one to understand the immunobiology of T. spiralis, highlighting the importance of eosinophils in the survival of the larva in skeletal muscle. Further studies are needed to characterize the cell populations that comprise the cellular infiltrate during the development of the mother cell.
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spelling pubmed-86176162021-11-27 Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice Vega-Sánchez, Vicente Gómez-De-Anda, Fabián-Ricardo Calderón-Domínguez, Georgina Ramírez-y-Ramírez, Mary-Carmen-del-Sol Reyes-Rodríguez, Nydia-E. Zepeda-Velázquez, Andrea-P. Tapia-Romero, Raquel de-la-Rosa-Arana, Jorge-Luis Pathogens Article We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of Trichinella spiralis (T. spiralis) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of the MSUS/MEX/91/CM-91 T. spiralis strain. Tongues and diaphragms were obtained daily from days 13 to 39 post infection. Diaphragms were compressed and subjected to Giemsa stain. Tongues were histologically sectioned and stained with erythrosine B or hematoxylin and eosin. The cellular infiltrate and the nurse cell-larva complex were detected by optical microscopy since day 16 post infection. The size of the larva increased exponentially during the course of the infection. The kinetics of eosinophils showed a multimodal trend, with a bimodal predominance. The maximum peaks were reached on days 21 and 27 post infection. The results of this study demonstrate that eosinophils occur abundantly in two transcendent moments of the T. spiralis life cycle: first, when the stage 1 larva invades the myocyte and second when the nurse cell-larva complex has been fully developed. These results help one to understand the immunobiology of T. spiralis, highlighting the importance of eosinophils in the survival of the larva in skeletal muscle. Further studies are needed to characterize the cell populations that comprise the cellular infiltrate during the development of the mother cell. MDPI 2021-10-26 /pmc/articles/PMC8617616/ /pubmed/34832538 http://dx.doi.org/10.3390/pathogens10111382 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vega-Sánchez, Vicente
Gómez-De-Anda, Fabián-Ricardo
Calderón-Domínguez, Georgina
Ramírez-y-Ramírez, Mary-Carmen-del-Sol
Reyes-Rodríguez, Nydia-E.
Zepeda-Velázquez, Andrea-P.
Tapia-Romero, Raquel
de-la-Rosa-Arana, Jorge-Luis
Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice
title Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice
title_full Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice
title_fullStr Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice
title_full_unstemmed Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice
title_short Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of Trichinella spiralis in Experimentally Infected Mice
title_sort kinetics of eosinophils during development of the cellular infiltrate surrounding the nurse cell of trichinella spiralis in experimentally infected mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617616/
https://www.ncbi.nlm.nih.gov/pubmed/34832538
http://dx.doi.org/10.3390/pathogens10111382
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