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Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea

Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the fae...

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Autores principales: Ji, Chengyuan, Zhang, Yao, Sun, Ruini, Ma, Jiale, Pan, Zihao, Yao, Huochun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617846/
https://www.ncbi.nlm.nih.gov/pubmed/34835023
http://dx.doi.org/10.3390/v13112217
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author Ji, Chengyuan
Zhang, Yao
Sun, Ruini
Ma, Jiale
Pan, Zihao
Yao, Huochun
author_facet Ji, Chengyuan
Zhang, Yao
Sun, Ruini
Ma, Jiale
Pan, Zihao
Yao, Huochun
author_sort Ji, Chengyuan
collection PubMed
description Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the faeces of cows with significant diarrhoeal symptoms in China and observed the viral particles within 20–30 nm through transmission electron microscopy. Then, we designated this strain as HB19-1 in this study. The multistep growth curves showed that the virus propagated well in the MDBK cells. Molecular genetic analysis of VP1 indicated that HB19-1 belonged to the BEV-F1 group. Although the challenged ICR mice did not exhibit typical disease symptoms in animal infection assay, we observed significant pathological damage in the lungs, intestines, and muscle tissues. In summary, we isolated a BEV strain HB19-1 causing severe diarrhoea in cattle and proposed reinforcing the epidemiological surveillance of this virus.
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spelling pubmed-86178462021-11-27 Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea Ji, Chengyuan Zhang, Yao Sun, Ruini Ma, Jiale Pan, Zihao Yao, Huochun Viruses Article Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the faeces of cows with significant diarrhoeal symptoms in China and observed the viral particles within 20–30 nm through transmission electron microscopy. Then, we designated this strain as HB19-1 in this study. The multistep growth curves showed that the virus propagated well in the MDBK cells. Molecular genetic analysis of VP1 indicated that HB19-1 belonged to the BEV-F1 group. Although the challenged ICR mice did not exhibit typical disease symptoms in animal infection assay, we observed significant pathological damage in the lungs, intestines, and muscle tissues. In summary, we isolated a BEV strain HB19-1 causing severe diarrhoea in cattle and proposed reinforcing the epidemiological surveillance of this virus. MDPI 2021-11-04 /pmc/articles/PMC8617846/ /pubmed/34835023 http://dx.doi.org/10.3390/v13112217 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ji, Chengyuan
Zhang, Yao
Sun, Ruini
Ma, Jiale
Pan, Zihao
Yao, Huochun
Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_full Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_fullStr Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_full_unstemmed Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_short Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
title_sort isolation and identification of type f bovine enterovirus from clinical cattle with diarrhoea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617846/
https://www.ncbi.nlm.nih.gov/pubmed/34835023
http://dx.doi.org/10.3390/v13112217
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