Cargando…
Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea
Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the fae...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617846/ https://www.ncbi.nlm.nih.gov/pubmed/34835023 http://dx.doi.org/10.3390/v13112217 |
_version_ | 1784604604842901504 |
---|---|
author | Ji, Chengyuan Zhang, Yao Sun, Ruini Ma, Jiale Pan, Zihao Yao, Huochun |
author_facet | Ji, Chengyuan Zhang, Yao Sun, Ruini Ma, Jiale Pan, Zihao Yao, Huochun |
author_sort | Ji, Chengyuan |
collection | PubMed |
description | Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the faeces of cows with significant diarrhoeal symptoms in China and observed the viral particles within 20–30 nm through transmission electron microscopy. Then, we designated this strain as HB19-1 in this study. The multistep growth curves showed that the virus propagated well in the MDBK cells. Molecular genetic analysis of VP1 indicated that HB19-1 belonged to the BEV-F1 group. Although the challenged ICR mice did not exhibit typical disease symptoms in animal infection assay, we observed significant pathological damage in the lungs, intestines, and muscle tissues. In summary, we isolated a BEV strain HB19-1 causing severe diarrhoea in cattle and proposed reinforcing the epidemiological surveillance of this virus. |
format | Online Article Text |
id | pubmed-8617846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86178462021-11-27 Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea Ji, Chengyuan Zhang, Yao Sun, Ruini Ma, Jiale Pan, Zihao Yao, Huochun Viruses Article Recently, bovine enterovirus (BEV) has caused several respiratory and gastrointestinal diseases outbreaks in cattle. Monitoring the epidemiological and pathogenic characteristics of this virus is crucial to controlling its spread. We isolated a BEV strain with typical cytopathic effects from the faeces of cows with significant diarrhoeal symptoms in China and observed the viral particles within 20–30 nm through transmission electron microscopy. Then, we designated this strain as HB19-1 in this study. The multistep growth curves showed that the virus propagated well in the MDBK cells. Molecular genetic analysis of VP1 indicated that HB19-1 belonged to the BEV-F1 group. Although the challenged ICR mice did not exhibit typical disease symptoms in animal infection assay, we observed significant pathological damage in the lungs, intestines, and muscle tissues. In summary, we isolated a BEV strain HB19-1 causing severe diarrhoea in cattle and proposed reinforcing the epidemiological surveillance of this virus. MDPI 2021-11-04 /pmc/articles/PMC8617846/ /pubmed/34835023 http://dx.doi.org/10.3390/v13112217 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ji, Chengyuan Zhang, Yao Sun, Ruini Ma, Jiale Pan, Zihao Yao, Huochun Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea |
title | Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea |
title_full | Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea |
title_fullStr | Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea |
title_full_unstemmed | Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea |
title_short | Isolation and Identification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea |
title_sort | isolation and identification of type f bovine enterovirus from clinical cattle with diarrhoea |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617846/ https://www.ncbi.nlm.nih.gov/pubmed/34835023 http://dx.doi.org/10.3390/v13112217 |
work_keys_str_mv | AT jichengyuan isolationandidentificationoftypefbovineenterovirusfromclinicalcattlewithdiarrhoea AT zhangyao isolationandidentificationoftypefbovineenterovirusfromclinicalcattlewithdiarrhoea AT sunruini isolationandidentificationoftypefbovineenterovirusfromclinicalcattlewithdiarrhoea AT majiale isolationandidentificationoftypefbovineenterovirusfromclinicalcattlewithdiarrhoea AT panzihao isolationandidentificationoftypefbovineenterovirusfromclinicalcattlewithdiarrhoea AT yaohuochun isolationandidentificationoftypefbovineenterovirusfromclinicalcattlewithdiarrhoea |