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Cytokine-Mediated Alterations of Human Cardiac Fibroblast’s Secretome
Fibroblasts contribute to approximately 20% of the non-cardiomyocytic cells in the heart. They play important roles in the myocardial adaption to stretch, inflammation, and other pathophysiological conditions. Fibroblasts are a major source of extracellular matrix (ECM) proteins whose production is...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617966/ https://www.ncbi.nlm.nih.gov/pubmed/34830141 http://dx.doi.org/10.3390/ijms222212262 |
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author | Bräuninger, Hanna Thottakara, Tilo Schön, Jacob Voss, Svenja Dhople, Vishnu Warnke, Svenja Scherschel, Katharina Schrage, Benedikt Kirchhof, Paulus Blankenberg, Stefan Völker, Uwe Westermann, Dirk Hammer, Elke Lindner, Diana |
author_facet | Bräuninger, Hanna Thottakara, Tilo Schön, Jacob Voss, Svenja Dhople, Vishnu Warnke, Svenja Scherschel, Katharina Schrage, Benedikt Kirchhof, Paulus Blankenberg, Stefan Völker, Uwe Westermann, Dirk Hammer, Elke Lindner, Diana |
author_sort | Bräuninger, Hanna |
collection | PubMed |
description | Fibroblasts contribute to approximately 20% of the non-cardiomyocytic cells in the heart. They play important roles in the myocardial adaption to stretch, inflammation, and other pathophysiological conditions. Fibroblasts are a major source of extracellular matrix (ECM) proteins whose production is regulated by cytokines, such as TNF-α or TGF-β. The resulting myocardial fibrosis is a hallmark of pathological remodeling in dilated cardiomyopathy (DCM). Therefore, in the present study, the secretome and corresponding transcriptome of human cardiac fibroblasts from patients with DCM was investigated under normal conditions and after TNF-α or TGF-β stimulation. Secreted proteins were quantified via mass spectrometry and expression of genes coding for secreted proteins was analyzed via Affymetrix Transcriptome Profiling. Thus, we provide comprehensive proteome and transcriptome data on the human cardiac fibroblast’s secretome. In the secretome of quiescent fibroblasts, 58% of the protein amount belonged to the ECM fraction. Interestingly, cytokines were responsible for 5% of the total protein amount in the secretome and up to 10% in the corresponding transcriptome. Furthermore, cytokine gene expression and secretion were upregulated upon TNF-α stimulation, while collagen secretion levels were elevated after TGF-β treatment. These results suggest that myocardial fibroblasts contribute to pro-fibrotic and to inflammatory processes in response to extracellular stimuli. |
format | Online Article Text |
id | pubmed-8617966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86179662021-11-27 Cytokine-Mediated Alterations of Human Cardiac Fibroblast’s Secretome Bräuninger, Hanna Thottakara, Tilo Schön, Jacob Voss, Svenja Dhople, Vishnu Warnke, Svenja Scherschel, Katharina Schrage, Benedikt Kirchhof, Paulus Blankenberg, Stefan Völker, Uwe Westermann, Dirk Hammer, Elke Lindner, Diana Int J Mol Sci Article Fibroblasts contribute to approximately 20% of the non-cardiomyocytic cells in the heart. They play important roles in the myocardial adaption to stretch, inflammation, and other pathophysiological conditions. Fibroblasts are a major source of extracellular matrix (ECM) proteins whose production is regulated by cytokines, such as TNF-α or TGF-β. The resulting myocardial fibrosis is a hallmark of pathological remodeling in dilated cardiomyopathy (DCM). Therefore, in the present study, the secretome and corresponding transcriptome of human cardiac fibroblasts from patients with DCM was investigated under normal conditions and after TNF-α or TGF-β stimulation. Secreted proteins were quantified via mass spectrometry and expression of genes coding for secreted proteins was analyzed via Affymetrix Transcriptome Profiling. Thus, we provide comprehensive proteome and transcriptome data on the human cardiac fibroblast’s secretome. In the secretome of quiescent fibroblasts, 58% of the protein amount belonged to the ECM fraction. Interestingly, cytokines were responsible for 5% of the total protein amount in the secretome and up to 10% in the corresponding transcriptome. Furthermore, cytokine gene expression and secretion were upregulated upon TNF-α stimulation, while collagen secretion levels were elevated after TGF-β treatment. These results suggest that myocardial fibroblasts contribute to pro-fibrotic and to inflammatory processes in response to extracellular stimuli. MDPI 2021-11-12 /pmc/articles/PMC8617966/ /pubmed/34830141 http://dx.doi.org/10.3390/ijms222212262 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bräuninger, Hanna Thottakara, Tilo Schön, Jacob Voss, Svenja Dhople, Vishnu Warnke, Svenja Scherschel, Katharina Schrage, Benedikt Kirchhof, Paulus Blankenberg, Stefan Völker, Uwe Westermann, Dirk Hammer, Elke Lindner, Diana Cytokine-Mediated Alterations of Human Cardiac Fibroblast’s Secretome |
title | Cytokine-Mediated Alterations of Human Cardiac Fibroblast’s Secretome |
title_full | Cytokine-Mediated Alterations of Human Cardiac Fibroblast’s Secretome |
title_fullStr | Cytokine-Mediated Alterations of Human Cardiac Fibroblast’s Secretome |
title_full_unstemmed | Cytokine-Mediated Alterations of Human Cardiac Fibroblast’s Secretome |
title_short | Cytokine-Mediated Alterations of Human Cardiac Fibroblast’s Secretome |
title_sort | cytokine-mediated alterations of human cardiac fibroblast’s secretome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617966/ https://www.ncbi.nlm.nih.gov/pubmed/34830141 http://dx.doi.org/10.3390/ijms222212262 |
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