Cargando…

A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths

Strains of Dunaliella salina microalgae are of considerable research and industrial interest because they hyper-accumulate β-carotene as well as produce high-quality protein. To explore the co-production of valuable compounds in D. salina, this study compared the production of β-carotene, phytoene a...

Descripción completa

Detalles Bibliográficos
Autores principales: Sui, Yixing, Mazzucchi, Laura, Acharya, Parag, Xu, Yanan, Morgan, Geraint, Harvey, Patricia J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617983/
https://www.ncbi.nlm.nih.gov/pubmed/34829102
http://dx.doi.org/10.3390/foods10112824
_version_ 1784604638114217984
author Sui, Yixing
Mazzucchi, Laura
Acharya, Parag
Xu, Yanan
Morgan, Geraint
Harvey, Patricia J.
author_facet Sui, Yixing
Mazzucchi, Laura
Acharya, Parag
Xu, Yanan
Morgan, Geraint
Harvey, Patricia J.
author_sort Sui, Yixing
collection PubMed
description Strains of Dunaliella salina microalgae are of considerable research and industrial interest because they hyper-accumulate β-carotene as well as produce high-quality protein. To explore the co-production of valuable compounds in D. salina, this study compared the production of β-carotene, phytoene and amino acids in two strains cultivated under white, red or blue light until no further nitrogen was available. D. salina DF15 (CCAP 19/41 (PLY DF15)) produced more than 12% β-carotene (ash-free dry weight (AFDW) basis), and red light triggered the production of 9-cis β-carotene at a 9-cis/all-trans β-carotene ratio of 1.5. Phytoene production was also evident in D. salina DF15 under all conditions, particularly under blue light. However, the profile of essential amino acids (EAAs) and calculation of the essential amino acid index (EAAI) was less than ideal in terms of protein quality, for both strains. Umami compounds, quantified as monosodium glutamate (MSG) equivalents, indicated a higher equivalent umami concentration (EUC) in D. salina DF15 under red light (3.2 g MSG/100 g AFDW) than in D. salina CCAP19/30. Overall, D. salina DF15 demonstrates valuable traits for further exploration and product optimisation.
format Online
Article
Text
id pubmed-8617983
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86179832021-11-27 A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths Sui, Yixing Mazzucchi, Laura Acharya, Parag Xu, Yanan Morgan, Geraint Harvey, Patricia J. Foods Article Strains of Dunaliella salina microalgae are of considerable research and industrial interest because they hyper-accumulate β-carotene as well as produce high-quality protein. To explore the co-production of valuable compounds in D. salina, this study compared the production of β-carotene, phytoene and amino acids in two strains cultivated under white, red or blue light until no further nitrogen was available. D. salina DF15 (CCAP 19/41 (PLY DF15)) produced more than 12% β-carotene (ash-free dry weight (AFDW) basis), and red light triggered the production of 9-cis β-carotene at a 9-cis/all-trans β-carotene ratio of 1.5. Phytoene production was also evident in D. salina DF15 under all conditions, particularly under blue light. However, the profile of essential amino acids (EAAs) and calculation of the essential amino acid index (EAAI) was less than ideal in terms of protein quality, for both strains. Umami compounds, quantified as monosodium glutamate (MSG) equivalents, indicated a higher equivalent umami concentration (EUC) in D. salina DF15 under red light (3.2 g MSG/100 g AFDW) than in D. salina CCAP19/30. Overall, D. salina DF15 demonstrates valuable traits for further exploration and product optimisation. MDPI 2021-11-16 /pmc/articles/PMC8617983/ /pubmed/34829102 http://dx.doi.org/10.3390/foods10112824 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sui, Yixing
Mazzucchi, Laura
Acharya, Parag
Xu, Yanan
Morgan, Geraint
Harvey, Patricia J.
A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths
title A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths
title_full A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths
title_fullStr A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths
title_full_unstemmed A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths
title_short A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths
title_sort comparison of β-carotene, phytoene and amino acids production in dunaliella salina df 15 (ccap 19/41) and dunaliella salina ccap 19/30 using different light wavelengths
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617983/
https://www.ncbi.nlm.nih.gov/pubmed/34829102
http://dx.doi.org/10.3390/foods10112824
work_keys_str_mv AT suiyixing acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT mazzucchilaura acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT acharyaparag acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT xuyanan acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT morgangeraint acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT harveypatriciaj acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT suiyixing comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT mazzucchilaura comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT acharyaparag comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT xuyanan comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT morgangeraint comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths
AT harveypatriciaj comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths