Cargando…
A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths
Strains of Dunaliella salina microalgae are of considerable research and industrial interest because they hyper-accumulate β-carotene as well as produce high-quality protein. To explore the co-production of valuable compounds in D. salina, this study compared the production of β-carotene, phytoene a...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617983/ https://www.ncbi.nlm.nih.gov/pubmed/34829102 http://dx.doi.org/10.3390/foods10112824 |
_version_ | 1784604638114217984 |
---|---|
author | Sui, Yixing Mazzucchi, Laura Acharya, Parag Xu, Yanan Morgan, Geraint Harvey, Patricia J. |
author_facet | Sui, Yixing Mazzucchi, Laura Acharya, Parag Xu, Yanan Morgan, Geraint Harvey, Patricia J. |
author_sort | Sui, Yixing |
collection | PubMed |
description | Strains of Dunaliella salina microalgae are of considerable research and industrial interest because they hyper-accumulate β-carotene as well as produce high-quality protein. To explore the co-production of valuable compounds in D. salina, this study compared the production of β-carotene, phytoene and amino acids in two strains cultivated under white, red or blue light until no further nitrogen was available. D. salina DF15 (CCAP 19/41 (PLY DF15)) produced more than 12% β-carotene (ash-free dry weight (AFDW) basis), and red light triggered the production of 9-cis β-carotene at a 9-cis/all-trans β-carotene ratio of 1.5. Phytoene production was also evident in D. salina DF15 under all conditions, particularly under blue light. However, the profile of essential amino acids (EAAs) and calculation of the essential amino acid index (EAAI) was less than ideal in terms of protein quality, for both strains. Umami compounds, quantified as monosodium glutamate (MSG) equivalents, indicated a higher equivalent umami concentration (EUC) in D. salina DF15 under red light (3.2 g MSG/100 g AFDW) than in D. salina CCAP19/30. Overall, D. salina DF15 demonstrates valuable traits for further exploration and product optimisation. |
format | Online Article Text |
id | pubmed-8617983 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86179832021-11-27 A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths Sui, Yixing Mazzucchi, Laura Acharya, Parag Xu, Yanan Morgan, Geraint Harvey, Patricia J. Foods Article Strains of Dunaliella salina microalgae are of considerable research and industrial interest because they hyper-accumulate β-carotene as well as produce high-quality protein. To explore the co-production of valuable compounds in D. salina, this study compared the production of β-carotene, phytoene and amino acids in two strains cultivated under white, red or blue light until no further nitrogen was available. D. salina DF15 (CCAP 19/41 (PLY DF15)) produced more than 12% β-carotene (ash-free dry weight (AFDW) basis), and red light triggered the production of 9-cis β-carotene at a 9-cis/all-trans β-carotene ratio of 1.5. Phytoene production was also evident in D. salina DF15 under all conditions, particularly under blue light. However, the profile of essential amino acids (EAAs) and calculation of the essential amino acid index (EAAI) was less than ideal in terms of protein quality, for both strains. Umami compounds, quantified as monosodium glutamate (MSG) equivalents, indicated a higher equivalent umami concentration (EUC) in D. salina DF15 under red light (3.2 g MSG/100 g AFDW) than in D. salina CCAP19/30. Overall, D. salina DF15 demonstrates valuable traits for further exploration and product optimisation. MDPI 2021-11-16 /pmc/articles/PMC8617983/ /pubmed/34829102 http://dx.doi.org/10.3390/foods10112824 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sui, Yixing Mazzucchi, Laura Acharya, Parag Xu, Yanan Morgan, Geraint Harvey, Patricia J. A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths |
title | A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths |
title_full | A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths |
title_fullStr | A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths |
title_full_unstemmed | A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths |
title_short | A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths |
title_sort | comparison of β-carotene, phytoene and amino acids production in dunaliella salina df 15 (ccap 19/41) and dunaliella salina ccap 19/30 using different light wavelengths |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617983/ https://www.ncbi.nlm.nih.gov/pubmed/34829102 http://dx.doi.org/10.3390/foods10112824 |
work_keys_str_mv | AT suiyixing acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT mazzucchilaura acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT acharyaparag acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT xuyanan acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT morgangeraint acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT harveypatriciaj acomparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT suiyixing comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT mazzucchilaura comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT acharyaparag comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT xuyanan comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT morgangeraint comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths AT harveypatriciaj comparisonofbcarotenephytoeneandaminoacidsproductionindunaliellasalinadf15ccap1941anddunaliellasalinaccap1930usingdifferentlightwavelengths |