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An Introductory Overview of Open-Source and Commercial Software Options for the Analysis of Forensic Sequencing Data

The top challenges of adopting new methods to forensic DNA analysis in routine laboratories are often the capital investment and the expertise required to implement and validate such methods locally. In the case of next-generation sequencing, in the last decade, several specifically forensic commerc...

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Detalles Bibliográficos
Autores principales: Huszar, Tunde I., Gettings, Katherine B., Vallone, Peter M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618049/
https://www.ncbi.nlm.nih.gov/pubmed/34828345
http://dx.doi.org/10.3390/genes12111739
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author Huszar, Tunde I.
Gettings, Katherine B.
Vallone, Peter M.
author_facet Huszar, Tunde I.
Gettings, Katherine B.
Vallone, Peter M.
author_sort Huszar, Tunde I.
collection PubMed
description The top challenges of adopting new methods to forensic DNA analysis in routine laboratories are often the capital investment and the expertise required to implement and validate such methods locally. In the case of next-generation sequencing, in the last decade, several specifically forensic commercial options became available, offering reliable and validated solutions. Despite this, the readily available expertise to analyze, interpret and understand such data is still perceived to be lagging behind. This review gives an introductory overview for the forensic scientists who are at the beginning of their journey with implementing next-generation sequencing locally and because most in the field do not have a bioinformatics background may find it difficult to navigate the new terms and analysis options available. The currently available open-source and commercial software for forensic sequencing data analysis are summarized here to provide an accessible starting point for those fairly new to the forensic application of massively parallel sequencing.
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spelling pubmed-86180492021-11-27 An Introductory Overview of Open-Source and Commercial Software Options for the Analysis of Forensic Sequencing Data Huszar, Tunde I. Gettings, Katherine B. Vallone, Peter M. Genes (Basel) Review The top challenges of adopting new methods to forensic DNA analysis in routine laboratories are often the capital investment and the expertise required to implement and validate such methods locally. In the case of next-generation sequencing, in the last decade, several specifically forensic commercial options became available, offering reliable and validated solutions. Despite this, the readily available expertise to analyze, interpret and understand such data is still perceived to be lagging behind. This review gives an introductory overview for the forensic scientists who are at the beginning of their journey with implementing next-generation sequencing locally and because most in the field do not have a bioinformatics background may find it difficult to navigate the new terms and analysis options available. The currently available open-source and commercial software for forensic sequencing data analysis are summarized here to provide an accessible starting point for those fairly new to the forensic application of massively parallel sequencing. MDPI 2021-10-29 /pmc/articles/PMC8618049/ /pubmed/34828345 http://dx.doi.org/10.3390/genes12111739 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Huszar, Tunde I.
Gettings, Katherine B.
Vallone, Peter M.
An Introductory Overview of Open-Source and Commercial Software Options for the Analysis of Forensic Sequencing Data
title An Introductory Overview of Open-Source and Commercial Software Options for the Analysis of Forensic Sequencing Data
title_full An Introductory Overview of Open-Source and Commercial Software Options for the Analysis of Forensic Sequencing Data
title_fullStr An Introductory Overview of Open-Source and Commercial Software Options for the Analysis of Forensic Sequencing Data
title_full_unstemmed An Introductory Overview of Open-Source and Commercial Software Options for the Analysis of Forensic Sequencing Data
title_short An Introductory Overview of Open-Source and Commercial Software Options for the Analysis of Forensic Sequencing Data
title_sort introductory overview of open-source and commercial software options for the analysis of forensic sequencing data
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618049/
https://www.ncbi.nlm.nih.gov/pubmed/34828345
http://dx.doi.org/10.3390/genes12111739
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