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Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China

Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse t...

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Autores principales: Yang, Xiuling, Du, Min, Li, Shupeng, Zhou, Xueping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618073/
https://www.ncbi.nlm.nih.gov/pubmed/34835016
http://dx.doi.org/10.3390/v13112210
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author Yang, Xiuling
Du, Min
Li, Shupeng
Zhou, Xueping
author_facet Yang, Xiuling
Du, Min
Li, Shupeng
Zhou, Xueping
author_sort Yang, Xiuling
collection PubMed
description Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse transcription PCR allowed the reconstruction of watermelon mosaic virus and an unknown virus. The genome of the unknown virus was determined to be 5870 nucleotides in length, and has a genomic organization with characteristic features of previously reported poleroviruses. Sequence analysis revealed that the virus was closely related to, but significantly different from, cotton leafroll dwarf virus, a polerovirus of the family Solemoviridae. This virus had less than 90% amino acid sequence identity in the products of both ORF0 and ORF1. According to the polerovirus species demarcation criteria set by the International Committee on Taxonomy of Viruses, this virus should be assigned to a new polerovirus species, for which we propose the name “cotton leaf roll virus”.
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spelling pubmed-86180732021-11-27 Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China Yang, Xiuling Du, Min Li, Shupeng Zhou, Xueping Viruses Article Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse transcription PCR allowed the reconstruction of watermelon mosaic virus and an unknown virus. The genome of the unknown virus was determined to be 5870 nucleotides in length, and has a genomic organization with characteristic features of previously reported poleroviruses. Sequence analysis revealed that the virus was closely related to, but significantly different from, cotton leafroll dwarf virus, a polerovirus of the family Solemoviridae. This virus had less than 90% amino acid sequence identity in the products of both ORF0 and ORF1. According to the polerovirus species demarcation criteria set by the International Committee on Taxonomy of Viruses, this virus should be assigned to a new polerovirus species, for which we propose the name “cotton leaf roll virus”. MDPI 2021-11-03 /pmc/articles/PMC8618073/ /pubmed/34835016 http://dx.doi.org/10.3390/v13112210 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Xiuling
Du, Min
Li, Shupeng
Zhou, Xueping
Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_full Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_fullStr Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_full_unstemmed Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_short Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
title_sort coinfection of cotton plants with watermelon mosaic virus and a novel polerovirus in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618073/
https://www.ncbi.nlm.nih.gov/pubmed/34835016
http://dx.doi.org/10.3390/v13112210
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