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Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China
Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618073/ https://www.ncbi.nlm.nih.gov/pubmed/34835016 http://dx.doi.org/10.3390/v13112210 |
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author | Yang, Xiuling Du, Min Li, Shupeng Zhou, Xueping |
author_facet | Yang, Xiuling Du, Min Li, Shupeng Zhou, Xueping |
author_sort | Yang, Xiuling |
collection | PubMed |
description | Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse transcription PCR allowed the reconstruction of watermelon mosaic virus and an unknown virus. The genome of the unknown virus was determined to be 5870 nucleotides in length, and has a genomic organization with characteristic features of previously reported poleroviruses. Sequence analysis revealed that the virus was closely related to, but significantly different from, cotton leafroll dwarf virus, a polerovirus of the family Solemoviridae. This virus had less than 90% amino acid sequence identity in the products of both ORF0 and ORF1. According to the polerovirus species demarcation criteria set by the International Committee on Taxonomy of Viruses, this virus should be assigned to a new polerovirus species, for which we propose the name “cotton leaf roll virus”. |
format | Online Article Text |
id | pubmed-8618073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86180732021-11-27 Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China Yang, Xiuling Du, Min Li, Shupeng Zhou, Xueping Viruses Article Cotton is the most important fiber crop worldwide. To determine the presence of viruses in cotton plants showing leaf roll and vein yellowing symptoms in Henan Province of China, a small RNA-based deep sequencing approach was performed. Analysis of the de novo-assembled contigs followed by reverse transcription PCR allowed the reconstruction of watermelon mosaic virus and an unknown virus. The genome of the unknown virus was determined to be 5870 nucleotides in length, and has a genomic organization with characteristic features of previously reported poleroviruses. Sequence analysis revealed that the virus was closely related to, but significantly different from, cotton leafroll dwarf virus, a polerovirus of the family Solemoviridae. This virus had less than 90% amino acid sequence identity in the products of both ORF0 and ORF1. According to the polerovirus species demarcation criteria set by the International Committee on Taxonomy of Viruses, this virus should be assigned to a new polerovirus species, for which we propose the name “cotton leaf roll virus”. MDPI 2021-11-03 /pmc/articles/PMC8618073/ /pubmed/34835016 http://dx.doi.org/10.3390/v13112210 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Xiuling Du, Min Li, Shupeng Zhou, Xueping Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China |
title | Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China |
title_full | Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China |
title_fullStr | Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China |
title_full_unstemmed | Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China |
title_short | Coinfection of Cotton Plants with Watermelon Mosaic Virus and a Novel Polerovirus in China |
title_sort | coinfection of cotton plants with watermelon mosaic virus and a novel polerovirus in china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618073/ https://www.ncbi.nlm.nih.gov/pubmed/34835016 http://dx.doi.org/10.3390/v13112210 |
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