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SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus

Biotrophic fungi have to infect their host to obtain nutrients and must establish an interaction with the host to complete their life cycle. In this process, effectors play important roles in manipulating the host’s immune system to avoid being attacked. Sporisorium scitamineum is the causative agen...

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Autores principales: Lu, Shan, Wang, Yukun, Shen, Xiaorui, Guo, Feng, Zhou, Chunling, Li, Ru, Chen, Baoshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618092/
https://www.ncbi.nlm.nih.gov/pubmed/34829241
http://dx.doi.org/10.3390/jof7110954
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author Lu, Shan
Wang, Yukun
Shen, Xiaorui
Guo, Feng
Zhou, Chunling
Li, Ru
Chen, Baoshan
author_facet Lu, Shan
Wang, Yukun
Shen, Xiaorui
Guo, Feng
Zhou, Chunling
Li, Ru
Chen, Baoshan
author_sort Lu, Shan
collection PubMed
description Biotrophic fungi have to infect their host to obtain nutrients and must establish an interaction with the host to complete their life cycle. In this process, effectors play important roles in manipulating the host’s immune system to avoid being attacked. Sporisorium scitamineum is the causative agent of sugarcane smut, the most important disease in sugarcane-producing regions worldwide. In this work, we functionally characterized the conserved effector PEP1 in S. scitamineum. The mating process and the expression of genes in the MAPK signaling pathway and the a and b loci were adversely affected in Sspep1-null mutants. The requirement for SsPEP1 in pathogenicity and symptom development was allele dosage-dependent, i.e., deleting one Sspep1 allele in the mating pair turned a normal black whip with abundant teliospores into a white whip with few teliospores; however, deleting both alleles almost abolished infectivity and whip development. ΔSspep1 mutants produced significantly less mycelium mass within infected plants. Additionally, SsPEP1 was identified as a potent inhibitor of sugarcane POD-1a peroxidase activity, implying that SsPEP1 may function to relieve reactive oxygen species-related stress within the host plant. Taken together, our work demonstrated that SsPEP1 is a multifaceted effector essential for S. scitamineum growth, development, and pathogenicity.
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spelling pubmed-86180922021-11-27 SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus Lu, Shan Wang, Yukun Shen, Xiaorui Guo, Feng Zhou, Chunling Li, Ru Chen, Baoshan J Fungi (Basel) Article Biotrophic fungi have to infect their host to obtain nutrients and must establish an interaction with the host to complete their life cycle. In this process, effectors play important roles in manipulating the host’s immune system to avoid being attacked. Sporisorium scitamineum is the causative agent of sugarcane smut, the most important disease in sugarcane-producing regions worldwide. In this work, we functionally characterized the conserved effector PEP1 in S. scitamineum. The mating process and the expression of genes in the MAPK signaling pathway and the a and b loci were adversely affected in Sspep1-null mutants. The requirement for SsPEP1 in pathogenicity and symptom development was allele dosage-dependent, i.e., deleting one Sspep1 allele in the mating pair turned a normal black whip with abundant teliospores into a white whip with few teliospores; however, deleting both alleles almost abolished infectivity and whip development. ΔSspep1 mutants produced significantly less mycelium mass within infected plants. Additionally, SsPEP1 was identified as a potent inhibitor of sugarcane POD-1a peroxidase activity, implying that SsPEP1 may function to relieve reactive oxygen species-related stress within the host plant. Taken together, our work demonstrated that SsPEP1 is a multifaceted effector essential for S. scitamineum growth, development, and pathogenicity. MDPI 2021-11-11 /pmc/articles/PMC8618092/ /pubmed/34829241 http://dx.doi.org/10.3390/jof7110954 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Shan
Wang, Yukun
Shen, Xiaorui
Guo, Feng
Zhou, Chunling
Li, Ru
Chen, Baoshan
SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_full SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_fullStr SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_full_unstemmed SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_short SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus
title_sort sspep1, an effector with essential cellular functions in sugarcane smut fungus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618092/
https://www.ncbi.nlm.nih.gov/pubmed/34829241
http://dx.doi.org/10.3390/jof7110954
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