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Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis

An ubiquinone derivative, pseudoalteromone A (1), has been isolated from two marine-derived Pseudoalteromonas spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrated by assessing the intracellular and e...

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Autores principales: Lim, Su-Jin, Min, Dae-jin, Kim, Sohee, Lee, Jihye, Lee, Eun-Soo, Kim, Hyuk, Cho, Sung-Yoen, Beak, Heung-Soo, Lee, Chang-Seok, Nam, Sang-Jip, Ko, Jaeyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618130/
https://www.ncbi.nlm.nih.gov/pubmed/34822483
http://dx.doi.org/10.3390/md19110612
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author Lim, Su-Jin
Min, Dae-jin
Kim, Sohee
Lee, Jihye
Lee, Eun-Soo
Kim, Hyuk
Cho, Sung-Yoen
Beak, Heung-Soo
Lee, Chang-Seok
Nam, Sang-Jip
Ko, Jaeyoung
author_facet Lim, Su-Jin
Min, Dae-jin
Kim, Sohee
Lee, Jihye
Lee, Eun-Soo
Kim, Hyuk
Cho, Sung-Yoen
Beak, Heung-Soo
Lee, Chang-Seok
Nam, Sang-Jip
Ko, Jaeyoung
author_sort Lim, Su-Jin
collection PubMed
description An ubiquinone derivative, pseudoalteromone A (1), has been isolated from two marine-derived Pseudoalteromonas spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrated by assessing the intracellular and extracellular melanin content and cellular tyrosinase activity in the B16 cell line, Melan-a mouse melanocyte cell line, and MNT-1 human malignant melanoma cell line. Treatment with pseudoalteromone A (40 μg/mL) for 72 h reduced α-melanocyte-stimulating hormone (α-MSH)-induced intracellular melanin production by up to 44.68% in B16 cells and 38.24% in MNT-1 cells. Notably, pseudoalteromone A induced a concentration-dependent reduction in cellular tyrosinase activity in B16 cell, and Western blot analyses showed that this inhibitory activity was associated with a significant decrease in protein levels of tyrosinase and tyrosinase-related protein 1 (Tyrp-1), suggesting that pseudoalteromone A exerts its anti-melanogenesis activity through effects on melanogenic genes. We further evaluated the skin-whitening effect of pseudoalteromone A in the three-dimensional (3D) pigmented-epidermis model, MelanoDerm, and visualized the 3D distribution of melanin by two-photon excited fluorescence imaging in this human skin equivalent. Collectively, our findings suggest that pseudoalteromone A inhibits tyrosinase activity and expression and that this accounts for its anti-melanogenic effects in melanocytes.
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spelling pubmed-86181302021-11-27 Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis Lim, Su-Jin Min, Dae-jin Kim, Sohee Lee, Jihye Lee, Eun-Soo Kim, Hyuk Cho, Sung-Yoen Beak, Heung-Soo Lee, Chang-Seok Nam, Sang-Jip Ko, Jaeyoung Mar Drugs Article An ubiquinone derivative, pseudoalteromone A (1), has been isolated from two marine-derived Pseudoalteromonas spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrated by assessing the intracellular and extracellular melanin content and cellular tyrosinase activity in the B16 cell line, Melan-a mouse melanocyte cell line, and MNT-1 human malignant melanoma cell line. Treatment with pseudoalteromone A (40 μg/mL) for 72 h reduced α-melanocyte-stimulating hormone (α-MSH)-induced intracellular melanin production by up to 44.68% in B16 cells and 38.24% in MNT-1 cells. Notably, pseudoalteromone A induced a concentration-dependent reduction in cellular tyrosinase activity in B16 cell, and Western blot analyses showed that this inhibitory activity was associated with a significant decrease in protein levels of tyrosinase and tyrosinase-related protein 1 (Tyrp-1), suggesting that pseudoalteromone A exerts its anti-melanogenesis activity through effects on melanogenic genes. We further evaluated the skin-whitening effect of pseudoalteromone A in the three-dimensional (3D) pigmented-epidermis model, MelanoDerm, and visualized the 3D distribution of melanin by two-photon excited fluorescence imaging in this human skin equivalent. Collectively, our findings suggest that pseudoalteromone A inhibits tyrosinase activity and expression and that this accounts for its anti-melanogenic effects in melanocytes. MDPI 2021-10-28 /pmc/articles/PMC8618130/ /pubmed/34822483 http://dx.doi.org/10.3390/md19110612 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lim, Su-Jin
Min, Dae-jin
Kim, Sohee
Lee, Jihye
Lee, Eun-Soo
Kim, Hyuk
Cho, Sung-Yoen
Beak, Heung-Soo
Lee, Chang-Seok
Nam, Sang-Jip
Ko, Jaeyoung
Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
title Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
title_full Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
title_fullStr Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
title_full_unstemmed Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
title_short Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
title_sort pseudoalteromone a, a ubiquinone derivative from marine pseudoalteromonas spp., suppresses melanogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618130/
https://www.ncbi.nlm.nih.gov/pubmed/34822483
http://dx.doi.org/10.3390/md19110612
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