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Polymer-Coated Nanoparticles for Therapeutic and Diagnostic Non-(10)B Enriched Polymer-Coated Boron Carbon Oxynitride (BCNO) Nanoparticles as Potent BNCT Drug
Boron neutron capture therapy (BNCT) is a powerful and selective anti-cancer therapy utilizing (10)B-enriched boron drugs. However, clinical advancement of BCNT is hampered by the insufficient loading of B-10 drugs throughout the solid tumor. Furthermore, the preparation of boron drugs for BNCT reli...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618246/ https://www.ncbi.nlm.nih.gov/pubmed/34835699 http://dx.doi.org/10.3390/nano11112936 |
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author | Chiang, Chen-Wei Chien, Yun-Chen Yu, Wen-Jui Ho, Chia-Yu Wang, Chih-Yi Wang, Tzu-Wei Chiang, Chi-Shiun Keng, Pei-Yuin |
author_facet | Chiang, Chen-Wei Chien, Yun-Chen Yu, Wen-Jui Ho, Chia-Yu Wang, Chih-Yi Wang, Tzu-Wei Chiang, Chi-Shiun Keng, Pei-Yuin |
author_sort | Chiang, Chen-Wei |
collection | PubMed |
description | Boron neutron capture therapy (BNCT) is a powerful and selective anti-cancer therapy utilizing (10)B-enriched boron drugs. However, clinical advancement of BCNT is hampered by the insufficient loading of B-10 drugs throughout the solid tumor. Furthermore, the preparation of boron drugs for BNCT relies on the use of the costly B-10 enriched precursor. To overcome these challenges, polymer-coated boron carbon oxynitride (BCNO) nanoparticles, with ~30% of boron, were developed with enhanced biocompatibility, cell uptake, and tumoricidal effect via BNCT. Using the ALTS1C1 cancer cell line, the IC(50) of the PEG@BCNO, bare, PEI@BCNO were determined to be 0.3 mg/mL, 0.1 mg/mL, and 0.05 mg/mL, respectively. As a proof-of-concept, the engineered non-(10)B enriched polymer-coated BCNO exhibited excellent anti-tumor effect via BNCT due to their high boron content per nanoparticle and due to the enhanced cellular internalization and retention compared to small molecular (10)B-BPA drug. The astrocytoma ALTS1C1 cells treated with bare, polyethyleneimine-, and polyethylene glycol-coated BCNO exhibited an acute cell death of 24, 37, and 43%, respectively, upon 30 min of neutron irradiation compared to the negligible cell death in PBS-treated and non-irradiated cells. The radical approach proposed in this study addresses the expensive and complex issues of B-10 isotope enrichment process; thus, enabling the preparation of boron drugs at a significantly lower cost, which will facilitate the development of boron drugs for BNCT. |
format | Online Article Text |
id | pubmed-8618246 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86182462021-11-27 Polymer-Coated Nanoparticles for Therapeutic and Diagnostic Non-(10)B Enriched Polymer-Coated Boron Carbon Oxynitride (BCNO) Nanoparticles as Potent BNCT Drug Chiang, Chen-Wei Chien, Yun-Chen Yu, Wen-Jui Ho, Chia-Yu Wang, Chih-Yi Wang, Tzu-Wei Chiang, Chi-Shiun Keng, Pei-Yuin Nanomaterials (Basel) Article Boron neutron capture therapy (BNCT) is a powerful and selective anti-cancer therapy utilizing (10)B-enriched boron drugs. However, clinical advancement of BCNT is hampered by the insufficient loading of B-10 drugs throughout the solid tumor. Furthermore, the preparation of boron drugs for BNCT relies on the use of the costly B-10 enriched precursor. To overcome these challenges, polymer-coated boron carbon oxynitride (BCNO) nanoparticles, with ~30% of boron, were developed with enhanced biocompatibility, cell uptake, and tumoricidal effect via BNCT. Using the ALTS1C1 cancer cell line, the IC(50) of the PEG@BCNO, bare, PEI@BCNO were determined to be 0.3 mg/mL, 0.1 mg/mL, and 0.05 mg/mL, respectively. As a proof-of-concept, the engineered non-(10)B enriched polymer-coated BCNO exhibited excellent anti-tumor effect via BNCT due to their high boron content per nanoparticle and due to the enhanced cellular internalization and retention compared to small molecular (10)B-BPA drug. The astrocytoma ALTS1C1 cells treated with bare, polyethyleneimine-, and polyethylene glycol-coated BCNO exhibited an acute cell death of 24, 37, and 43%, respectively, upon 30 min of neutron irradiation compared to the negligible cell death in PBS-treated and non-irradiated cells. The radical approach proposed in this study addresses the expensive and complex issues of B-10 isotope enrichment process; thus, enabling the preparation of boron drugs at a significantly lower cost, which will facilitate the development of boron drugs for BNCT. MDPI 2021-11-02 /pmc/articles/PMC8618246/ /pubmed/34835699 http://dx.doi.org/10.3390/nano11112936 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chiang, Chen-Wei Chien, Yun-Chen Yu, Wen-Jui Ho, Chia-Yu Wang, Chih-Yi Wang, Tzu-Wei Chiang, Chi-Shiun Keng, Pei-Yuin Polymer-Coated Nanoparticles for Therapeutic and Diagnostic Non-(10)B Enriched Polymer-Coated Boron Carbon Oxynitride (BCNO) Nanoparticles as Potent BNCT Drug |
title | Polymer-Coated Nanoparticles for Therapeutic and Diagnostic Non-(10)B Enriched Polymer-Coated Boron Carbon Oxynitride (BCNO) Nanoparticles as Potent BNCT Drug |
title_full | Polymer-Coated Nanoparticles for Therapeutic and Diagnostic Non-(10)B Enriched Polymer-Coated Boron Carbon Oxynitride (BCNO) Nanoparticles as Potent BNCT Drug |
title_fullStr | Polymer-Coated Nanoparticles for Therapeutic and Diagnostic Non-(10)B Enriched Polymer-Coated Boron Carbon Oxynitride (BCNO) Nanoparticles as Potent BNCT Drug |
title_full_unstemmed | Polymer-Coated Nanoparticles for Therapeutic and Diagnostic Non-(10)B Enriched Polymer-Coated Boron Carbon Oxynitride (BCNO) Nanoparticles as Potent BNCT Drug |
title_short | Polymer-Coated Nanoparticles for Therapeutic and Diagnostic Non-(10)B Enriched Polymer-Coated Boron Carbon Oxynitride (BCNO) Nanoparticles as Potent BNCT Drug |
title_sort | polymer-coated nanoparticles for therapeutic and diagnostic non-(10)b enriched polymer-coated boron carbon oxynitride (bcno) nanoparticles as potent bnct drug |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618246/ https://www.ncbi.nlm.nih.gov/pubmed/34835699 http://dx.doi.org/10.3390/nano11112936 |
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