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Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation

This study describes the preparation and evaluation of two formulations, a hydrogel and a nanostructured system, containing ketorolac tromethamine as an anti-inflammatory agent for the local therapy against the inflammatory process derived from the surgical excision of Condyloma acuminata. Both form...

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Autores principales: El Moussaoui, Salima, Abo-Horan, Ismael, Halbaut, Lyda, Alonso, Cristina, Coderch, Lluïsa, Garduño-Ramírez, María Luisa, Clares, Beatriz, Soriano, José Luis, Calpena, Ana Cristina, Fernández-Campos, Francisco, Mallandrich, Mireia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618351/
https://www.ncbi.nlm.nih.gov/pubmed/34834198
http://dx.doi.org/10.3390/pharmaceutics13111784
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author El Moussaoui, Salima
Abo-Horan, Ismael
Halbaut, Lyda
Alonso, Cristina
Coderch, Lluïsa
Garduño-Ramírez, María Luisa
Clares, Beatriz
Soriano, José Luis
Calpena, Ana Cristina
Fernández-Campos, Francisco
Mallandrich, Mireia
author_facet El Moussaoui, Salima
Abo-Horan, Ismael
Halbaut, Lyda
Alonso, Cristina
Coderch, Lluïsa
Garduño-Ramírez, María Luisa
Clares, Beatriz
Soriano, José Luis
Calpena, Ana Cristina
Fernández-Campos, Francisco
Mallandrich, Mireia
author_sort El Moussaoui, Salima
collection PubMed
description This study describes the preparation and evaluation of two formulations, a hydrogel and a nanostructured system, containing ketorolac tromethamine as an anti-inflammatory agent for the local therapy against the inflammatory process derived from the surgical excision of Condyloma acuminata. Both formulations were physicochemically characterized. In vitro release profiles show that the nanoparticles release 92% ± 2.3 of the total ketorolac tromethamine encapsulated, while the chitosan gel releases 18.6% ± 0.2. The ex vivo permeation and distribution through human skin were also assayed and was observed how the main amount of ketorolac tromethamine is retained in the epidermis. In vivo studies were accomplished to evaluate the anti-inflammatory efficacy in mice which also involved the histological analysis to confirm the in vivo results. The nanoparticles present a significantly higher anti-inflammatory efficacy than chitosan gel. The tolerability of developed formulations was assessed by monitoring the biomechanical properties of the skin before and after application of both formulations. No statistical differences in trans-epidermal water loss and skin hydration with respect to the basal values were observed and the formulations exhibited higher anti-inflammatory activity compared to a reference ketotorlac tromethamine solution. Therefore, it can be concluded that both formulations can be proposed as outstanding candidates for offering a local anti-inflammatory therapeutical tool with potential clinical application.
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spelling pubmed-86183512021-11-27 Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation El Moussaoui, Salima Abo-Horan, Ismael Halbaut, Lyda Alonso, Cristina Coderch, Lluïsa Garduño-Ramírez, María Luisa Clares, Beatriz Soriano, José Luis Calpena, Ana Cristina Fernández-Campos, Francisco Mallandrich, Mireia Pharmaceutics Article This study describes the preparation and evaluation of two formulations, a hydrogel and a nanostructured system, containing ketorolac tromethamine as an anti-inflammatory agent for the local therapy against the inflammatory process derived from the surgical excision of Condyloma acuminata. Both formulations were physicochemically characterized. In vitro release profiles show that the nanoparticles release 92% ± 2.3 of the total ketorolac tromethamine encapsulated, while the chitosan gel releases 18.6% ± 0.2. The ex vivo permeation and distribution through human skin were also assayed and was observed how the main amount of ketorolac tromethamine is retained in the epidermis. In vivo studies were accomplished to evaluate the anti-inflammatory efficacy in mice which also involved the histological analysis to confirm the in vivo results. The nanoparticles present a significantly higher anti-inflammatory efficacy than chitosan gel. The tolerability of developed formulations was assessed by monitoring the biomechanical properties of the skin before and after application of both formulations. No statistical differences in trans-epidermal water loss and skin hydration with respect to the basal values were observed and the formulations exhibited higher anti-inflammatory activity compared to a reference ketotorlac tromethamine solution. Therefore, it can be concluded that both formulations can be proposed as outstanding candidates for offering a local anti-inflammatory therapeutical tool with potential clinical application. MDPI 2021-10-25 /pmc/articles/PMC8618351/ /pubmed/34834198 http://dx.doi.org/10.3390/pharmaceutics13111784 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
El Moussaoui, Salima
Abo-Horan, Ismael
Halbaut, Lyda
Alonso, Cristina
Coderch, Lluïsa
Garduño-Ramírez, María Luisa
Clares, Beatriz
Soriano, José Luis
Calpena, Ana Cristina
Fernández-Campos, Francisco
Mallandrich, Mireia
Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_full Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_fullStr Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_full_unstemmed Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_short Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_sort polymeric nanoparticles and chitosan gel loading ketorolac tromethamine to alleviate pain associated with condyloma acuminata during the pre- and post-ablation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618351/
https://www.ncbi.nlm.nih.gov/pubmed/34834198
http://dx.doi.org/10.3390/pharmaceutics13111784
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