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Latest Advances in Protein-Recovery Technologies from Agricultural Waste

In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural w...

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Autores principales: Yusree, Farhana Iylia Fatinee Mohd, Peter, Angela Paul, Mohd Nor, Mohd Zuhair, Show, Pau Loke, Mokhtar, Mohd Noriznan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618363/
https://www.ncbi.nlm.nih.gov/pubmed/34829028
http://dx.doi.org/10.3390/foods10112748
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author Yusree, Farhana Iylia Fatinee Mohd
Peter, Angela Paul
Mohd Nor, Mohd Zuhair
Show, Pau Loke
Mokhtar, Mohd Noriznan
author_facet Yusree, Farhana Iylia Fatinee Mohd
Peter, Angela Paul
Mohd Nor, Mohd Zuhair
Show, Pau Loke
Mokhtar, Mohd Noriznan
author_sort Yusree, Farhana Iylia Fatinee Mohd
collection PubMed
description In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques.
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spelling pubmed-86183632021-11-27 Latest Advances in Protein-Recovery Technologies from Agricultural Waste Yusree, Farhana Iylia Fatinee Mohd Peter, Angela Paul Mohd Nor, Mohd Zuhair Show, Pau Loke Mokhtar, Mohd Noriznan Foods Review In recent years, downstream bioprocessing industries are venturing into less tedious, simple, and high-efficiency separation by implementing advanced purification and extraction methods. This review discusses the separation of proteins, with the main focus on amylase as an enzyme from agricultural waste using conventional and advanced techniques of extraction and purification via a liquid biphasic system (LBS). In comparison to other methods, such as membrane extraction, precipitation, ultrasonication, and chromatography, the LBS stands out as an efficient, cost-effective, and adaptable developing method for protein recovery. The two-phase separation method can be water-soluble polymers, or polymer and salt, or alcohol and salt, which is a simpler and lower-cost method that can be used at a larger purification scale. The comparison of different approaches in LBS for amylase purification from agricultural waste is also included. Current technology has evolved from a simple LBS into microwave-assisted LBS, liquid biphasic flotation (LBF), thermoseparation (TMP), three-phase partitioning (TPP), ultrasound-assisted LBS, and electrically assisted LBS. pH, time, temperature, and concentration are some of the significant research parameters considered in the review of advanced techniques. MDPI 2021-11-09 /pmc/articles/PMC8618363/ /pubmed/34829028 http://dx.doi.org/10.3390/foods10112748 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Yusree, Farhana Iylia Fatinee Mohd
Peter, Angela Paul
Mohd Nor, Mohd Zuhair
Show, Pau Loke
Mokhtar, Mohd Noriznan
Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_full Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_fullStr Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_full_unstemmed Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_short Latest Advances in Protein-Recovery Technologies from Agricultural Waste
title_sort latest advances in protein-recovery technologies from agricultural waste
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618363/
https://www.ncbi.nlm.nih.gov/pubmed/34829028
http://dx.doi.org/10.3390/foods10112748
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