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Development of Hydrolysis Probe-Based qPCR Assays for Panax ginseng and Panax quinquefolius for Detection of Adulteration in Ginseng Herbal Products

Authentication of Panax ginseng and Panax quinquefolius products is important to be able to mitigate instances of adulteration and substitution that exist within the international supply chain of ginseng. To address this issue, species-specific hydrolysis probe qPCR assays were developed and validat...

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Autores principales: Kesanakurti, Prasad, Ragupathy, Subramanyam, Faller, Adam C., Shanmughanandhan, Dhivya, Buongiorno, Francesco, Della Noce, Isabella, Lu, Zhengfei, Zhang, Yanjun, Newmaster, Steven G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618564/
https://www.ncbi.nlm.nih.gov/pubmed/34828986
http://dx.doi.org/10.3390/foods10112705
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author Kesanakurti, Prasad
Ragupathy, Subramanyam
Faller, Adam C.
Shanmughanandhan, Dhivya
Buongiorno, Francesco
Della Noce, Isabella
Lu, Zhengfei
Zhang, Yanjun
Newmaster, Steven G.
author_facet Kesanakurti, Prasad
Ragupathy, Subramanyam
Faller, Adam C.
Shanmughanandhan, Dhivya
Buongiorno, Francesco
Della Noce, Isabella
Lu, Zhengfei
Zhang, Yanjun
Newmaster, Steven G.
author_sort Kesanakurti, Prasad
collection PubMed
description Authentication of Panax ginseng and Panax quinquefolius products is important to be able to mitigate instances of adulteration and substitution that exist within the international supply chain of ginseng. To address this issue, species-specific hydrolysis probe qPCR assays were developed and validated for both P. ginseng and P. quinquefolius herbal dietary supplements. Performance of the probe-based assays was evaluated using analytical validation criteria, which included evaluation of: (1) specificity, in selectively identifying the target species; (2) sensitivity, in detecting the lowest amount of the target material; and (3) repeatability and reproducibility of the method in detecting the target species in raw materials on a real-time PCR platform (reliability). The species-specific probes were developed and successfully passed the validation criteria with 100% specificity, 80–120% efficiency and 100% reliability. The methods developed in this study are fit for purpose, rapid, and easy to implement in quality assurance programs; authentication of ginseng herbal supplements is possible, even with extracts where DNA is fragmented and of low quality and quantity.
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spelling pubmed-86185642021-11-27 Development of Hydrolysis Probe-Based qPCR Assays for Panax ginseng and Panax quinquefolius for Detection of Adulteration in Ginseng Herbal Products Kesanakurti, Prasad Ragupathy, Subramanyam Faller, Adam C. Shanmughanandhan, Dhivya Buongiorno, Francesco Della Noce, Isabella Lu, Zhengfei Zhang, Yanjun Newmaster, Steven G. Foods Article Authentication of Panax ginseng and Panax quinquefolius products is important to be able to mitigate instances of adulteration and substitution that exist within the international supply chain of ginseng. To address this issue, species-specific hydrolysis probe qPCR assays were developed and validated for both P. ginseng and P. quinquefolius herbal dietary supplements. Performance of the probe-based assays was evaluated using analytical validation criteria, which included evaluation of: (1) specificity, in selectively identifying the target species; (2) sensitivity, in detecting the lowest amount of the target material; and (3) repeatability and reproducibility of the method in detecting the target species in raw materials on a real-time PCR platform (reliability). The species-specific probes were developed and successfully passed the validation criteria with 100% specificity, 80–120% efficiency and 100% reliability. The methods developed in this study are fit for purpose, rapid, and easy to implement in quality assurance programs; authentication of ginseng herbal supplements is possible, even with extracts where DNA is fragmented and of low quality and quantity. MDPI 2021-11-05 /pmc/articles/PMC8618564/ /pubmed/34828986 http://dx.doi.org/10.3390/foods10112705 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kesanakurti, Prasad
Ragupathy, Subramanyam
Faller, Adam C.
Shanmughanandhan, Dhivya
Buongiorno, Francesco
Della Noce, Isabella
Lu, Zhengfei
Zhang, Yanjun
Newmaster, Steven G.
Development of Hydrolysis Probe-Based qPCR Assays for Panax ginseng and Panax quinquefolius for Detection of Adulteration in Ginseng Herbal Products
title Development of Hydrolysis Probe-Based qPCR Assays for Panax ginseng and Panax quinquefolius for Detection of Adulteration in Ginseng Herbal Products
title_full Development of Hydrolysis Probe-Based qPCR Assays for Panax ginseng and Panax quinquefolius for Detection of Adulteration in Ginseng Herbal Products
title_fullStr Development of Hydrolysis Probe-Based qPCR Assays for Panax ginseng and Panax quinquefolius for Detection of Adulteration in Ginseng Herbal Products
title_full_unstemmed Development of Hydrolysis Probe-Based qPCR Assays for Panax ginseng and Panax quinquefolius for Detection of Adulteration in Ginseng Herbal Products
title_short Development of Hydrolysis Probe-Based qPCR Assays for Panax ginseng and Panax quinquefolius for Detection of Adulteration in Ginseng Herbal Products
title_sort development of hydrolysis probe-based qpcr assays for panax ginseng and panax quinquefolius for detection of adulteration in ginseng herbal products
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618564/
https://www.ncbi.nlm.nih.gov/pubmed/34828986
http://dx.doi.org/10.3390/foods10112705
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