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The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta)

Unicellular volvocalean green algal Haematococcus pluvialis, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders the extraction of as...

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Autores principales: Guo, Chunli, Mei, Rui, Anwar, Muhammad, Zhao, Di, Lan, Chengxiang, Jiang, Yanan, Zhuang, Jieyi, Wang, Chaogang, Hu, Zhangli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618704/
https://www.ncbi.nlm.nih.gov/pubmed/34822383
http://dx.doi.org/10.3390/metabo11110725
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author Guo, Chunli
Mei, Rui
Anwar, Muhammad
Zhao, Di
Lan, Chengxiang
Jiang, Yanan
Zhuang, Jieyi
Wang, Chaogang
Hu, Zhangli
author_facet Guo, Chunli
Mei, Rui
Anwar, Muhammad
Zhao, Di
Lan, Chengxiang
Jiang, Yanan
Zhuang, Jieyi
Wang, Chaogang
Hu, Zhangli
author_sort Guo, Chunli
collection PubMed
description Unicellular volvocalean green algal Haematococcus pluvialis, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders the extraction of astaxanthin and the genetic manipulation is still unclear. In this study, the cell wall components under salicylic acid and high light stresses were explored, and cellulose was considered the main component in the flagellates, which changed gradually into mannose in the aplanospore stages. During the period, the genes related to the cellulose and mannose metabolisms were identified based on the RNA-seq data, which presented a similar expression pattern. The positive correlations were observed among these studied genes by Pearson Correlation (PC) analysis, indicating the coordination between pathways of cellulose and mannose metabolism. The study firstly explored the formation mechanism of aplanospore cell wall, which might be of scientific significance in the study of H. pluvialis.
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spelling pubmed-86187042021-11-27 The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta) Guo, Chunli Mei, Rui Anwar, Muhammad Zhao, Di Lan, Chengxiang Jiang, Yanan Zhuang, Jieyi Wang, Chaogang Hu, Zhangli Metabolites Article Unicellular volvocalean green algal Haematococcus pluvialis, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders the extraction of astaxanthin and the genetic manipulation is still unclear. In this study, the cell wall components under salicylic acid and high light stresses were explored, and cellulose was considered the main component in the flagellates, which changed gradually into mannose in the aplanospore stages. During the period, the genes related to the cellulose and mannose metabolisms were identified based on the RNA-seq data, which presented a similar expression pattern. The positive correlations were observed among these studied genes by Pearson Correlation (PC) analysis, indicating the coordination between pathways of cellulose and mannose metabolism. The study firstly explored the formation mechanism of aplanospore cell wall, which might be of scientific significance in the study of H. pluvialis. MDPI 2021-10-23 /pmc/articles/PMC8618704/ /pubmed/34822383 http://dx.doi.org/10.3390/metabo11110725 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Chunli
Mei, Rui
Anwar, Muhammad
Zhao, Di
Lan, Chengxiang
Jiang, Yanan
Zhuang, Jieyi
Wang, Chaogang
Hu, Zhangli
The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta)
title The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta)
title_full The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta)
title_fullStr The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta)
title_full_unstemmed The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta)
title_short The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta)
title_sort functionally characterization of putative genes involved in the formation of mannose in the aplanospore cell wall of haematococcus pluvialis (volvocales, chlorophyta)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618704/
https://www.ncbi.nlm.nih.gov/pubmed/34822383
http://dx.doi.org/10.3390/metabo11110725
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