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The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta)
Unicellular volvocalean green algal Haematococcus pluvialis, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders the extraction of as...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618704/ https://www.ncbi.nlm.nih.gov/pubmed/34822383 http://dx.doi.org/10.3390/metabo11110725 |
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author | Guo, Chunli Mei, Rui Anwar, Muhammad Zhao, Di Lan, Chengxiang Jiang, Yanan Zhuang, Jieyi Wang, Chaogang Hu, Zhangli |
author_facet | Guo, Chunli Mei, Rui Anwar, Muhammad Zhao, Di Lan, Chengxiang Jiang, Yanan Zhuang, Jieyi Wang, Chaogang Hu, Zhangli |
author_sort | Guo, Chunli |
collection | PubMed |
description | Unicellular volvocalean green algal Haematococcus pluvialis, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders the extraction of astaxanthin and the genetic manipulation is still unclear. In this study, the cell wall components under salicylic acid and high light stresses were explored, and cellulose was considered the main component in the flagellates, which changed gradually into mannose in the aplanospore stages. During the period, the genes related to the cellulose and mannose metabolisms were identified based on the RNA-seq data, which presented a similar expression pattern. The positive correlations were observed among these studied genes by Pearson Correlation (PC) analysis, indicating the coordination between pathways of cellulose and mannose metabolism. The study firstly explored the formation mechanism of aplanospore cell wall, which might be of scientific significance in the study of H. pluvialis. |
format | Online Article Text |
id | pubmed-8618704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86187042021-11-27 The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta) Guo, Chunli Mei, Rui Anwar, Muhammad Zhao, Di Lan, Chengxiang Jiang, Yanan Zhuang, Jieyi Wang, Chaogang Hu, Zhangli Metabolites Article Unicellular volvocalean green algal Haematococcus pluvialis, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders the extraction of astaxanthin and the genetic manipulation is still unclear. In this study, the cell wall components under salicylic acid and high light stresses were explored, and cellulose was considered the main component in the flagellates, which changed gradually into mannose in the aplanospore stages. During the period, the genes related to the cellulose and mannose metabolisms were identified based on the RNA-seq data, which presented a similar expression pattern. The positive correlations were observed among these studied genes by Pearson Correlation (PC) analysis, indicating the coordination between pathways of cellulose and mannose metabolism. The study firstly explored the formation mechanism of aplanospore cell wall, which might be of scientific significance in the study of H. pluvialis. MDPI 2021-10-23 /pmc/articles/PMC8618704/ /pubmed/34822383 http://dx.doi.org/10.3390/metabo11110725 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guo, Chunli Mei, Rui Anwar, Muhammad Zhao, Di Lan, Chengxiang Jiang, Yanan Zhuang, Jieyi Wang, Chaogang Hu, Zhangli The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta) |
title | The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta) |
title_full | The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta) |
title_fullStr | The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta) |
title_full_unstemmed | The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta) |
title_short | The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta) |
title_sort | functionally characterization of putative genes involved in the formation of mannose in the aplanospore cell wall of haematococcus pluvialis (volvocales, chlorophyta) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618704/ https://www.ncbi.nlm.nih.gov/pubmed/34822383 http://dx.doi.org/10.3390/metabo11110725 |
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