CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions

CRISPR/Cas is a powerful tool for studying the role of genes in viral infections. The invention of CRISPR screening technologies has made it possible to untangle complex interactions between the host and viral agents. Moreover, whole-genome and pathway-specific CRISPR screens have facilitated identi...

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Autores principales: Chulanov, Vladimir, Kostyusheva, Anastasiya, Brezgin, Sergey, Ponomareva, Natalia, Gegechkori, Vladimir, Volchkova, Elena, Pimenov, Nikolay, Kostyushev, Dmitry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618713/
https://www.ncbi.nlm.nih.gov/pubmed/34835064
http://dx.doi.org/10.3390/v13112258
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author Chulanov, Vladimir
Kostyusheva, Anastasiya
Brezgin, Sergey
Ponomareva, Natalia
Gegechkori, Vladimir
Volchkova, Elena
Pimenov, Nikolay
Kostyushev, Dmitry
author_facet Chulanov, Vladimir
Kostyusheva, Anastasiya
Brezgin, Sergey
Ponomareva, Natalia
Gegechkori, Vladimir
Volchkova, Elena
Pimenov, Nikolay
Kostyushev, Dmitry
author_sort Chulanov, Vladimir
collection PubMed
description CRISPR/Cas is a powerful tool for studying the role of genes in viral infections. The invention of CRISPR screening technologies has made it possible to untangle complex interactions between the host and viral agents. Moreover, whole-genome and pathway-specific CRISPR screens have facilitated identification of novel drug candidates for treating viral infections. In this review, we highlight recent developments in the fields of CRISPR/Cas with a focus on the use of CRISPR screens for studying viral infections and identifying new candidate genes to aid development of antivirals.
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spelling pubmed-86187132021-11-27 CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions Chulanov, Vladimir Kostyusheva, Anastasiya Brezgin, Sergey Ponomareva, Natalia Gegechkori, Vladimir Volchkova, Elena Pimenov, Nikolay Kostyushev, Dmitry Viruses Review CRISPR/Cas is a powerful tool for studying the role of genes in viral infections. The invention of CRISPR screening technologies has made it possible to untangle complex interactions between the host and viral agents. Moreover, whole-genome and pathway-specific CRISPR screens have facilitated identification of novel drug candidates for treating viral infections. In this review, we highlight recent developments in the fields of CRISPR/Cas with a focus on the use of CRISPR screens for studying viral infections and identifying new candidate genes to aid development of antivirals. MDPI 2021-11-11 /pmc/articles/PMC8618713/ /pubmed/34835064 http://dx.doi.org/10.3390/v13112258 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Chulanov, Vladimir
Kostyusheva, Anastasiya
Brezgin, Sergey
Ponomareva, Natalia
Gegechkori, Vladimir
Volchkova, Elena
Pimenov, Nikolay
Kostyushev, Dmitry
CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions
title CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions
title_full CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions
title_fullStr CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions
title_full_unstemmed CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions
title_short CRISPR Screening: Molecular Tools for Studying Virus–Host Interactions
title_sort crispr screening: molecular tools for studying virus–host interactions
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618713/
https://www.ncbi.nlm.nih.gov/pubmed/34835064
http://dx.doi.org/10.3390/v13112258
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