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Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models

The objective of the study was to compare the effects of experimentally induced type 1 or type 2 diabetes (T1D or T2D) on the functional, structural and biochemical properties of mouse peripheral nerves. Eight-week-old C57BL/6 mice were randomly assigned into three groups, including the control (CTR...

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Autores principales: Jaroslawska, Julia, Korytko, Agnieszka, Zglejc-Waszak, Kamila, Antonowski, Tomasz, Pomianowski, Andrzej S., Wasowicz, Krzysztof, Wojtkiewicz, Joanna, Juranek, Judyta K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618965/
https://www.ncbi.nlm.nih.gov/pubmed/34833143
http://dx.doi.org/10.3390/life11111267
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author Jaroslawska, Julia
Korytko, Agnieszka
Zglejc-Waszak, Kamila
Antonowski, Tomasz
Pomianowski, Andrzej S.
Wasowicz, Krzysztof
Wojtkiewicz, Joanna
Juranek, Judyta K.
author_facet Jaroslawska, Julia
Korytko, Agnieszka
Zglejc-Waszak, Kamila
Antonowski, Tomasz
Pomianowski, Andrzej S.
Wasowicz, Krzysztof
Wojtkiewicz, Joanna
Juranek, Judyta K.
author_sort Jaroslawska, Julia
collection PubMed
description The objective of the study was to compare the effects of experimentally induced type 1 or type 2 diabetes (T1D or T2D) on the functional, structural and biochemical properties of mouse peripheral nerves. Eight-week-old C57BL/6 mice were randomly assigned into three groups, including the control (CTRL, chow-fed), STZ (streptozotocin (STZ)-injected), and HFD (high-fat diet (HFD)-fed) group. After 18-weeks of experimental treatment, HFD mice had higher body weights and elevated levels of plasma lipids, while STZ mice developed hyperglycemia. STZ-treated mice, after an extended period of untreated diabetes, developed motor and sensory nerve conduction-velocity deficits. Moreover, relative to control fibers, pre- and diabetic axons were lower in number and irregular in shape. Animals from both treatment groups manifested a pronounced overexpression of nNOS and a reduced expression of SOD1 proteins in the sciatic nerve, indicating oxidative–nitrosative stress and ineffective antioxidant protection in the peripheral nervous system of these mice. Collectively, STZ- and HFD-treated mice revealed similar characteristics of peripheral nerve damage, including a number of morphological and electrophysiological pathologies in the sciatic nerve. While hyperglycemia is a large component of diabetic neuropathy pathogenesis, the non-hyperglycemic effects of diabetes, including dyslipidemia, may also be of importance in the development of this condition.
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spelling pubmed-86189652021-11-27 Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models Jaroslawska, Julia Korytko, Agnieszka Zglejc-Waszak, Kamila Antonowski, Tomasz Pomianowski, Andrzej S. Wasowicz, Krzysztof Wojtkiewicz, Joanna Juranek, Judyta K. Life (Basel) Article The objective of the study was to compare the effects of experimentally induced type 1 or type 2 diabetes (T1D or T2D) on the functional, structural and biochemical properties of mouse peripheral nerves. Eight-week-old C57BL/6 mice were randomly assigned into three groups, including the control (CTRL, chow-fed), STZ (streptozotocin (STZ)-injected), and HFD (high-fat diet (HFD)-fed) group. After 18-weeks of experimental treatment, HFD mice had higher body weights and elevated levels of plasma lipids, while STZ mice developed hyperglycemia. STZ-treated mice, after an extended period of untreated diabetes, developed motor and sensory nerve conduction-velocity deficits. Moreover, relative to control fibers, pre- and diabetic axons were lower in number and irregular in shape. Animals from both treatment groups manifested a pronounced overexpression of nNOS and a reduced expression of SOD1 proteins in the sciatic nerve, indicating oxidative–nitrosative stress and ineffective antioxidant protection in the peripheral nervous system of these mice. Collectively, STZ- and HFD-treated mice revealed similar characteristics of peripheral nerve damage, including a number of morphological and electrophysiological pathologies in the sciatic nerve. While hyperglycemia is a large component of diabetic neuropathy pathogenesis, the non-hyperglycemic effects of diabetes, including dyslipidemia, may also be of importance in the development of this condition. MDPI 2021-11-19 /pmc/articles/PMC8618965/ /pubmed/34833143 http://dx.doi.org/10.3390/life11111267 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jaroslawska, Julia
Korytko, Agnieszka
Zglejc-Waszak, Kamila
Antonowski, Tomasz
Pomianowski, Andrzej S.
Wasowicz, Krzysztof
Wojtkiewicz, Joanna
Juranek, Judyta K.
Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_full Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_fullStr Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_full_unstemmed Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_short Peripheral Neuropathy Presents Similar Symptoms and Pathological Changes in Both High-Fat Diet and Pharmacologically Induced Pre- and Diabetic Mouse Models
title_sort peripheral neuropathy presents similar symptoms and pathological changes in both high-fat diet and pharmacologically induced pre- and diabetic mouse models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8618965/
https://www.ncbi.nlm.nih.gov/pubmed/34833143
http://dx.doi.org/10.3390/life11111267
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