Cargando…

Multi-Omics Reveals the Inhibition of Lactiplantibacillus plantarum CCFM8724 in Streptococcus mutans-Candida albicans Mixed-Species Biofilms

Lactiplantibacillus plantarum CCFM8724 is a probiotic with the potential to prevent dental caries in vitro and in vivo. To explore the effects of this probiotic at inhibiting Streptococcus mutans-Candida albicans mixed-species biofilm and preventing dental caries, multi-omics, including metabolomics...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Qiuxiang, Li, Jiaxun, Lu, Wenwei, Zhao, Jianxin, Zhang, Hao, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619341/
https://www.ncbi.nlm.nih.gov/pubmed/34835493
http://dx.doi.org/10.3390/microorganisms9112368
_version_ 1784604967968964608
author Zhang, Qiuxiang
Li, Jiaxun
Lu, Wenwei
Zhao, Jianxin
Zhang, Hao
Chen, Wei
author_facet Zhang, Qiuxiang
Li, Jiaxun
Lu, Wenwei
Zhao, Jianxin
Zhang, Hao
Chen, Wei
author_sort Zhang, Qiuxiang
collection PubMed
description Lactiplantibacillus plantarum CCFM8724 is a probiotic with the potential to prevent dental caries in vitro and in vivo. To explore the effects of this probiotic at inhibiting Streptococcus mutans-Candida albicans mixed-species biofilm and preventing dental caries, multi-omics, including metabolomics and transcriptomics, was used to investigate the regulation of small-molecule metabolism during biofilm formation and the gene expression in the mixed-species biofilm. Metabolomic analysis revealed that some carbohydrates related to biofilm formation, such as sucrose, was detected at lower levels due to the treatment with the L. plantarum supernatant. Some sugar alcohols, such as xylitol and sorbitol, were detected at higher levels, which may have inhibited the growth of S. mutans. In transcriptomic analysis, the expression of the virulence genes of C. albicans, such as those that code agglutinin-like sequence (Als) proteins, was affected. In addition, metabolomics coupled with a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and RNA-seq revealed that the L. plantarum supernatant had an active role in sugar metabolism during the formation of the S. mutans-C. albicans mixed-species biofilm, and the L. plantarum supernatant was also related to carbohydrate utilization, glucan biosynthesis, and mycelium formation. Hence, L. plantarum CCFM8724 decreased the mixed-species biofilm mass from the perspective of gene expression and metabolic reprogramming. Our results provide a rationale for evaluating L. plantarum CCFM8724 as a potential oral probiotic for inhibiting cariogenic pathogen biofilm formation and improving dental caries.
format Online
Article
Text
id pubmed-8619341
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86193412021-11-27 Multi-Omics Reveals the Inhibition of Lactiplantibacillus plantarum CCFM8724 in Streptococcus mutans-Candida albicans Mixed-Species Biofilms Zhang, Qiuxiang Li, Jiaxun Lu, Wenwei Zhao, Jianxin Zhang, Hao Chen, Wei Microorganisms Article Lactiplantibacillus plantarum CCFM8724 is a probiotic with the potential to prevent dental caries in vitro and in vivo. To explore the effects of this probiotic at inhibiting Streptococcus mutans-Candida albicans mixed-species biofilm and preventing dental caries, multi-omics, including metabolomics and transcriptomics, was used to investigate the regulation of small-molecule metabolism during biofilm formation and the gene expression in the mixed-species biofilm. Metabolomic analysis revealed that some carbohydrates related to biofilm formation, such as sucrose, was detected at lower levels due to the treatment with the L. plantarum supernatant. Some sugar alcohols, such as xylitol and sorbitol, were detected at higher levels, which may have inhibited the growth of S. mutans. In transcriptomic analysis, the expression of the virulence genes of C. albicans, such as those that code agglutinin-like sequence (Als) proteins, was affected. In addition, metabolomics coupled with a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and RNA-seq revealed that the L. plantarum supernatant had an active role in sugar metabolism during the formation of the S. mutans-C. albicans mixed-species biofilm, and the L. plantarum supernatant was also related to carbohydrate utilization, glucan biosynthesis, and mycelium formation. Hence, L. plantarum CCFM8724 decreased the mixed-species biofilm mass from the perspective of gene expression and metabolic reprogramming. Our results provide a rationale for evaluating L. plantarum CCFM8724 as a potential oral probiotic for inhibiting cariogenic pathogen biofilm formation and improving dental caries. MDPI 2021-11-16 /pmc/articles/PMC8619341/ /pubmed/34835493 http://dx.doi.org/10.3390/microorganisms9112368 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Qiuxiang
Li, Jiaxun
Lu, Wenwei
Zhao, Jianxin
Zhang, Hao
Chen, Wei
Multi-Omics Reveals the Inhibition of Lactiplantibacillus plantarum CCFM8724 in Streptococcus mutans-Candida albicans Mixed-Species Biofilms
title Multi-Omics Reveals the Inhibition of Lactiplantibacillus plantarum CCFM8724 in Streptococcus mutans-Candida albicans Mixed-Species Biofilms
title_full Multi-Omics Reveals the Inhibition of Lactiplantibacillus plantarum CCFM8724 in Streptococcus mutans-Candida albicans Mixed-Species Biofilms
title_fullStr Multi-Omics Reveals the Inhibition of Lactiplantibacillus plantarum CCFM8724 in Streptococcus mutans-Candida albicans Mixed-Species Biofilms
title_full_unstemmed Multi-Omics Reveals the Inhibition of Lactiplantibacillus plantarum CCFM8724 in Streptococcus mutans-Candida albicans Mixed-Species Biofilms
title_short Multi-Omics Reveals the Inhibition of Lactiplantibacillus plantarum CCFM8724 in Streptococcus mutans-Candida albicans Mixed-Species Biofilms
title_sort multi-omics reveals the inhibition of lactiplantibacillus plantarum ccfm8724 in streptococcus mutans-candida albicans mixed-species biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619341/
https://www.ncbi.nlm.nih.gov/pubmed/34835493
http://dx.doi.org/10.3390/microorganisms9112368
work_keys_str_mv AT zhangqiuxiang multiomicsrevealstheinhibitionoflactiplantibacillusplantarumccfm8724instreptococcusmutanscandidaalbicansmixedspeciesbiofilms
AT lijiaxun multiomicsrevealstheinhibitionoflactiplantibacillusplantarumccfm8724instreptococcusmutanscandidaalbicansmixedspeciesbiofilms
AT luwenwei multiomicsrevealstheinhibitionoflactiplantibacillusplantarumccfm8724instreptococcusmutanscandidaalbicansmixedspeciesbiofilms
AT zhaojianxin multiomicsrevealstheinhibitionoflactiplantibacillusplantarumccfm8724instreptococcusmutanscandidaalbicansmixedspeciesbiofilms
AT zhanghao multiomicsrevealstheinhibitionoflactiplantibacillusplantarumccfm8724instreptococcusmutanscandidaalbicansmixedspeciesbiofilms
AT chenwei multiomicsrevealstheinhibitionoflactiplantibacillusplantarumccfm8724instreptococcusmutanscandidaalbicansmixedspeciesbiofilms