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Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant

Small animal models are of crucial importance for assessing COVID-19 countermeasures. Common laboratory mice would be well-suited for this purpose but are not susceptible to infection with wild-type SARS-CoV-2. However, the development of mouse-adapted virus strains has revealed key mutations in the...

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Autores principales: Kant, Ravi, Kareinen, Lauri, Smura, Teemu, Freitag, Tobias L., Jha, Sawan Kumar, Alitalo, Kari, Meri, Seppo, Sironen, Tarja, Saksela, Kalle, Strandin, Tomas, Kipar, Anja, Vapalahti, Olli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619350/
https://www.ncbi.nlm.nih.gov/pubmed/34835069
http://dx.doi.org/10.3390/v13112263
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author Kant, Ravi
Kareinen, Lauri
Smura, Teemu
Freitag, Tobias L.
Jha, Sawan Kumar
Alitalo, Kari
Meri, Seppo
Sironen, Tarja
Saksela, Kalle
Strandin, Tomas
Kipar, Anja
Vapalahti, Olli
author_facet Kant, Ravi
Kareinen, Lauri
Smura, Teemu
Freitag, Tobias L.
Jha, Sawan Kumar
Alitalo, Kari
Meri, Seppo
Sironen, Tarja
Saksela, Kalle
Strandin, Tomas
Kipar, Anja
Vapalahti, Olli
author_sort Kant, Ravi
collection PubMed
description Small animal models are of crucial importance for assessing COVID-19 countermeasures. Common laboratory mice would be well-suited for this purpose but are not susceptible to infection with wild-type SARS-CoV-2. However, the development of mouse-adapted virus strains has revealed key mutations in the SARS-CoV-2 spike protein that increase infectivity, and interestingly, many of these mutations are also present in naturally occurring SARS-CoV-2 variants of concern. This suggests that these variants might have the ability to infect common laboratory mice. Herein we show that the SARS-CoV-2 beta variant attains infectibility to BALB/c mice and causes pulmonary changes within 2–3 days post infection, consistent with results seen in other murine models of COVID-19, at a reasonable virus dose (2 × 10(5) PFU). The findings suggest that common laboratory mice can serve as the animal model of choice for testing the effectiveness of antiviral drugs and vaccines against SARS-CoV-2.
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spelling pubmed-86193502021-11-27 Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant Kant, Ravi Kareinen, Lauri Smura, Teemu Freitag, Tobias L. Jha, Sawan Kumar Alitalo, Kari Meri, Seppo Sironen, Tarja Saksela, Kalle Strandin, Tomas Kipar, Anja Vapalahti, Olli Viruses Article Small animal models are of crucial importance for assessing COVID-19 countermeasures. Common laboratory mice would be well-suited for this purpose but are not susceptible to infection with wild-type SARS-CoV-2. However, the development of mouse-adapted virus strains has revealed key mutations in the SARS-CoV-2 spike protein that increase infectivity, and interestingly, many of these mutations are also present in naturally occurring SARS-CoV-2 variants of concern. This suggests that these variants might have the ability to infect common laboratory mice. Herein we show that the SARS-CoV-2 beta variant attains infectibility to BALB/c mice and causes pulmonary changes within 2–3 days post infection, consistent with results seen in other murine models of COVID-19, at a reasonable virus dose (2 × 10(5) PFU). The findings suggest that common laboratory mice can serve as the animal model of choice for testing the effectiveness of antiviral drugs and vaccines against SARS-CoV-2. MDPI 2021-11-11 /pmc/articles/PMC8619350/ /pubmed/34835069 http://dx.doi.org/10.3390/v13112263 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kant, Ravi
Kareinen, Lauri
Smura, Teemu
Freitag, Tobias L.
Jha, Sawan Kumar
Alitalo, Kari
Meri, Seppo
Sironen, Tarja
Saksela, Kalle
Strandin, Tomas
Kipar, Anja
Vapalahti, Olli
Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant
title Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant
title_full Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant
title_fullStr Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant
title_full_unstemmed Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant
title_short Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant
title_sort common laboratory mice are susceptible to infection with the sars-cov-2 beta variant
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619350/
https://www.ncbi.nlm.nih.gov/pubmed/34835069
http://dx.doi.org/10.3390/v13112263
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