A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria

Information on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of t...

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Autores principales: Palombieri, Andrea, Tsachev, Ilia, Sarchese, Vittorio, Fruci, Paola, Di Profio, Federica, Pepovich, Roman, Baymakova, Magdalena, Marsilio, Fulvio, Martella, Vito, Di Martino, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619608/
https://www.ncbi.nlm.nih.gov/pubmed/34822640
http://dx.doi.org/10.3390/vetsci8110267
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author Palombieri, Andrea
Tsachev, Ilia
Sarchese, Vittorio
Fruci, Paola
Di Profio, Federica
Pepovich, Roman
Baymakova, Magdalena
Marsilio, Fulvio
Martella, Vito
Di Martino, Barbara
author_facet Palombieri, Andrea
Tsachev, Ilia
Sarchese, Vittorio
Fruci, Paola
Di Profio, Federica
Pepovich, Roman
Baymakova, Magdalena
Marsilio, Fulvio
Martella, Vito
Di Martino, Barbara
author_sort Palombieri, Andrea
collection PubMed
description Information on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of the partial polymerase (RdRp) region revealed the highest genetic correlation with HEVs of genotype (Gt) 3 identified in French and Dutch patients. For three such strains, a 700-bp fragment of the open reading frame 2 gene was generated. On phylogenetic analysis, the Bulgarian strains clustered tightly (93.8–98.3% nt) with human and animal HEVs classified within the Gt3 subtype c.
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spelling pubmed-86196082021-11-27 A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria Palombieri, Andrea Tsachev, Ilia Sarchese, Vittorio Fruci, Paola Di Profio, Federica Pepovich, Roman Baymakova, Magdalena Marsilio, Fulvio Martella, Vito Di Martino, Barbara Vet Sci Communication Information on hepatitis E virus (HEV) strains circulating in animal reservoirs in Bulgaria is currently lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of the partial polymerase (RdRp) region revealed the highest genetic correlation with HEVs of genotype (Gt) 3 identified in French and Dutch patients. For three such strains, a 700-bp fragment of the open reading frame 2 gene was generated. On phylogenetic analysis, the Bulgarian strains clustered tightly (93.8–98.3% nt) with human and animal HEVs classified within the Gt3 subtype c. MDPI 2021-11-06 /pmc/articles/PMC8619608/ /pubmed/34822640 http://dx.doi.org/10.3390/vetsci8110267 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Palombieri, Andrea
Tsachev, Ilia
Sarchese, Vittorio
Fruci, Paola
Di Profio, Federica
Pepovich, Roman
Baymakova, Magdalena
Marsilio, Fulvio
Martella, Vito
Di Martino, Barbara
A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_full A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_fullStr A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_full_unstemmed A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_short A Molecular Study on Hepatitis E Virus (HEV) in Pigs in Bulgaria
title_sort molecular study on hepatitis e virus (hev) in pigs in bulgaria
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619608/
https://www.ncbi.nlm.nih.gov/pubmed/34822640
http://dx.doi.org/10.3390/vetsci8110267
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