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Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients

Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in mo...

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Autores principales: Pavlova, Ina P., Nair, Sujit S., Lundon, Dara, Sobotka, Stanislaw, Roshandel, Reza, Treacy, Patrick-Julien, Ratnani, Parita, Brody, Rachel, Epstein, Jonathan I., Ayala, Gustavo E., Kyprianou, Natasha, Tewari, Ashutosh K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619628/
https://www.ncbi.nlm.nih.gov/pubmed/34834413
http://dx.doi.org/10.3390/jpm11111061
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author Pavlova, Ina P.
Nair, Sujit S.
Lundon, Dara
Sobotka, Stanislaw
Roshandel, Reza
Treacy, Patrick-Julien
Ratnani, Parita
Brody, Rachel
Epstein, Jonathan I.
Ayala, Gustavo E.
Kyprianou, Natasha
Tewari, Ashutosh K.
author_facet Pavlova, Ina P.
Nair, Sujit S.
Lundon, Dara
Sobotka, Stanislaw
Roshandel, Reza
Treacy, Patrick-Julien
Ratnani, Parita
Brody, Rachel
Epstein, Jonathan I.
Ayala, Gustavo E.
Kyprianou, Natasha
Tewari, Ashutosh K.
author_sort Pavlova, Ina P.
collection PubMed
description Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in modulating prostate tumor behavior and in predicting post-surgical outcomes. However, the emergence of stromal signatures has been limited, due in part to the lack of adoption of imaging modalities for stromal-specific profiling. Herein, label-free multiphoton microscopy (MPM), with its ability to image tissue with stromal-specific contrast, is used to identify prostate stromal features associated with aggressive tumor behavior and clinical outcome. MPM was performed on unstained prostatectomy specimens from 59 patients and on biopsy specimens from 17 patients with known post-surgery recurrence status. MPM-identified collagen content, organization, and morphological tumor signatures were extracted for each patient and screened for association with recurrent disease. Compared to tumors from patients whose disease did not recur, tumors from patients with recurrent disease exhibited higher MPM-identified collagen amount and collagen fiber intensity signal and width. Our study shows an association between MPM-identified stromal collagen features of prostate tumors and post-surgical disease recurrence, suggesting their potential for prostate cancer risk assessment.
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spelling pubmed-86196282021-11-27 Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients Pavlova, Ina P. Nair, Sujit S. Lundon, Dara Sobotka, Stanislaw Roshandel, Reza Treacy, Patrick-Julien Ratnani, Parita Brody, Rachel Epstein, Jonathan I. Ayala, Gustavo E. Kyprianou, Natasha Tewari, Ashutosh K. J Pers Med Article Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in modulating prostate tumor behavior and in predicting post-surgical outcomes. However, the emergence of stromal signatures has been limited, due in part to the lack of adoption of imaging modalities for stromal-specific profiling. Herein, label-free multiphoton microscopy (MPM), with its ability to image tissue with stromal-specific contrast, is used to identify prostate stromal features associated with aggressive tumor behavior and clinical outcome. MPM was performed on unstained prostatectomy specimens from 59 patients and on biopsy specimens from 17 patients with known post-surgery recurrence status. MPM-identified collagen content, organization, and morphological tumor signatures were extracted for each patient and screened for association with recurrent disease. Compared to tumors from patients whose disease did not recur, tumors from patients with recurrent disease exhibited higher MPM-identified collagen amount and collagen fiber intensity signal and width. Our study shows an association between MPM-identified stromal collagen features of prostate tumors and post-surgical disease recurrence, suggesting their potential for prostate cancer risk assessment. MDPI 2021-10-22 /pmc/articles/PMC8619628/ /pubmed/34834413 http://dx.doi.org/10.3390/jpm11111061 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pavlova, Ina P.
Nair, Sujit S.
Lundon, Dara
Sobotka, Stanislaw
Roshandel, Reza
Treacy, Patrick-Julien
Ratnani, Parita
Brody, Rachel
Epstein, Jonathan I.
Ayala, Gustavo E.
Kyprianou, Natasha
Tewari, Ashutosh K.
Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_full Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_fullStr Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_full_unstemmed Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_short Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
title_sort multiphoton microscopy for identifying collagen signatures associated with biochemical recurrence in prostate cancer patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619628/
https://www.ncbi.nlm.nih.gov/pubmed/34834413
http://dx.doi.org/10.3390/jpm11111061
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