Cargando…
Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in mo...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619628/ https://www.ncbi.nlm.nih.gov/pubmed/34834413 http://dx.doi.org/10.3390/jpm11111061 |
_version_ | 1784605039656960000 |
---|---|
author | Pavlova, Ina P. Nair, Sujit S. Lundon, Dara Sobotka, Stanislaw Roshandel, Reza Treacy, Patrick-Julien Ratnani, Parita Brody, Rachel Epstein, Jonathan I. Ayala, Gustavo E. Kyprianou, Natasha Tewari, Ashutosh K. |
author_facet | Pavlova, Ina P. Nair, Sujit S. Lundon, Dara Sobotka, Stanislaw Roshandel, Reza Treacy, Patrick-Julien Ratnani, Parita Brody, Rachel Epstein, Jonathan I. Ayala, Gustavo E. Kyprianou, Natasha Tewari, Ashutosh K. |
author_sort | Pavlova, Ina P. |
collection | PubMed |
description | Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in modulating prostate tumor behavior and in predicting post-surgical outcomes. However, the emergence of stromal signatures has been limited, due in part to the lack of adoption of imaging modalities for stromal-specific profiling. Herein, label-free multiphoton microscopy (MPM), with its ability to image tissue with stromal-specific contrast, is used to identify prostate stromal features associated with aggressive tumor behavior and clinical outcome. MPM was performed on unstained prostatectomy specimens from 59 patients and on biopsy specimens from 17 patients with known post-surgery recurrence status. MPM-identified collagen content, organization, and morphological tumor signatures were extracted for each patient and screened for association with recurrent disease. Compared to tumors from patients whose disease did not recur, tumors from patients with recurrent disease exhibited higher MPM-identified collagen amount and collagen fiber intensity signal and width. Our study shows an association between MPM-identified stromal collagen features of prostate tumors and post-surgical disease recurrence, suggesting their potential for prostate cancer risk assessment. |
format | Online Article Text |
id | pubmed-8619628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86196282021-11-27 Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients Pavlova, Ina P. Nair, Sujit S. Lundon, Dara Sobotka, Stanislaw Roshandel, Reza Treacy, Patrick-Julien Ratnani, Parita Brody, Rachel Epstein, Jonathan I. Ayala, Gustavo E. Kyprianou, Natasha Tewari, Ashutosh K. J Pers Med Article Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in modulating prostate tumor behavior and in predicting post-surgical outcomes. However, the emergence of stromal signatures has been limited, due in part to the lack of adoption of imaging modalities for stromal-specific profiling. Herein, label-free multiphoton microscopy (MPM), with its ability to image tissue with stromal-specific contrast, is used to identify prostate stromal features associated with aggressive tumor behavior and clinical outcome. MPM was performed on unstained prostatectomy specimens from 59 patients and on biopsy specimens from 17 patients with known post-surgery recurrence status. MPM-identified collagen content, organization, and morphological tumor signatures were extracted for each patient and screened for association with recurrent disease. Compared to tumors from patients whose disease did not recur, tumors from patients with recurrent disease exhibited higher MPM-identified collagen amount and collagen fiber intensity signal and width. Our study shows an association between MPM-identified stromal collagen features of prostate tumors and post-surgical disease recurrence, suggesting their potential for prostate cancer risk assessment. MDPI 2021-10-22 /pmc/articles/PMC8619628/ /pubmed/34834413 http://dx.doi.org/10.3390/jpm11111061 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pavlova, Ina P. Nair, Sujit S. Lundon, Dara Sobotka, Stanislaw Roshandel, Reza Treacy, Patrick-Julien Ratnani, Parita Brody, Rachel Epstein, Jonathan I. Ayala, Gustavo E. Kyprianou, Natasha Tewari, Ashutosh K. Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients |
title | Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients |
title_full | Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients |
title_fullStr | Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients |
title_full_unstemmed | Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients |
title_short | Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients |
title_sort | multiphoton microscopy for identifying collagen signatures associated with biochemical recurrence in prostate cancer patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619628/ https://www.ncbi.nlm.nih.gov/pubmed/34834413 http://dx.doi.org/10.3390/jpm11111061 |
work_keys_str_mv | AT pavlovainap multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT nairsujits multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT lundondara multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT sobotkastanislaw multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT roshandelreza multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT treacypatrickjulien multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT ratnaniparita multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT brodyrachel multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT epsteinjonathani multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT ayalagustavoe multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT kyprianounatasha multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients AT tewariashutoshk multiphotonmicroscopyforidentifyingcollagensignaturesassociatedwithbiochemicalrecurrenceinprostatecancerpatients |