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Commensal Bifidobacterium Strains Enhance the Efficacy of Neo-Epitope Based Cancer Vaccines

A large body of data both in animals and humans demonstrates that the gut microbiome plays a fundamental role in cancer immunity and in determining the efficacy of cancer immunotherapy. In this work, we have investigated whether and to what extent the gut microbiome can influence the antitumor activ...

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Autores principales: Tomasi, Michele, Dalsass, Mattia, Beghini, Francesco, Zanella, Ilaria, Caproni, Elena, Fantappiè, Laura, Gagliardi, Assunta, Irene, Carmela, König, Enrico, Frattini, Luca, Masetti, Giulia, Isaac, Samine Jessica, Armanini, Federica, Cumbo, Fabio, Blanco-Míguez, Aitor, Grandi, Alberto, Segata, Nicola, Grandi, Guido
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619961/
https://www.ncbi.nlm.nih.gov/pubmed/34835287
http://dx.doi.org/10.3390/vaccines9111356
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author Tomasi, Michele
Dalsass, Mattia
Beghini, Francesco
Zanella, Ilaria
Caproni, Elena
Fantappiè, Laura
Gagliardi, Assunta
Irene, Carmela
König, Enrico
Frattini, Luca
Masetti, Giulia
Isaac, Samine Jessica
Armanini, Federica
Cumbo, Fabio
Blanco-Míguez, Aitor
Grandi, Alberto
Segata, Nicola
Grandi, Guido
author_facet Tomasi, Michele
Dalsass, Mattia
Beghini, Francesco
Zanella, Ilaria
Caproni, Elena
Fantappiè, Laura
Gagliardi, Assunta
Irene, Carmela
König, Enrico
Frattini, Luca
Masetti, Giulia
Isaac, Samine Jessica
Armanini, Federica
Cumbo, Fabio
Blanco-Míguez, Aitor
Grandi, Alberto
Segata, Nicola
Grandi, Guido
author_sort Tomasi, Michele
collection PubMed
description A large body of data both in animals and humans demonstrates that the gut microbiome plays a fundamental role in cancer immunity and in determining the efficacy of cancer immunotherapy. In this work, we have investigated whether and to what extent the gut microbiome can influence the antitumor activity of neo-epitope-based cancer vaccines in a BALB/c-CT26 cancer mouse model. Similarly to that observed in the C57BL/6-B16 model, Bifidobacterium administration per se has a beneficial effect on CT26 tumor inhibition. Furthermore, the combination of Bifidobacterium administration and vaccination resulted in a protection which was superior to vaccination alone and to Bifidobacterium administration alone, and correlated with an increase in the frequency of vaccine-specific T cells. The gut microbiome analysis by 16S rRNA gene sequencing and shotgun metagenomics showed that tumor challenge rapidly altered the microbiome population, with Muribaculaceae being enriched and Lachnospiraceae being reduced. Over time, the population of Muribaculaceae progressively reduced while the Lachnospiraceae population increased—a trend that appeared to be retarded by the oral administration of Bifidobacterium. Interestingly, in some Bacteroidales, Prevotella and Muribaculacee species we identified sequences highly homologous to immunogenic neo-epitopes of CT26 cells, supporting the possible role of “molecular mimicry” in anticancer immunity. Our data strengthen the importance of the microbiome in cancer immunity and suggests a microbiome-based strategy to potentiate neo-epitope-based cancer vaccines.
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spelling pubmed-86199612021-11-27 Commensal Bifidobacterium Strains Enhance the Efficacy of Neo-Epitope Based Cancer Vaccines Tomasi, Michele Dalsass, Mattia Beghini, Francesco Zanella, Ilaria Caproni, Elena Fantappiè, Laura Gagliardi, Assunta Irene, Carmela König, Enrico Frattini, Luca Masetti, Giulia Isaac, Samine Jessica Armanini, Federica Cumbo, Fabio Blanco-Míguez, Aitor Grandi, Alberto Segata, Nicola Grandi, Guido Vaccines (Basel) Article A large body of data both in animals and humans demonstrates that the gut microbiome plays a fundamental role in cancer immunity and in determining the efficacy of cancer immunotherapy. In this work, we have investigated whether and to what extent the gut microbiome can influence the antitumor activity of neo-epitope-based cancer vaccines in a BALB/c-CT26 cancer mouse model. Similarly to that observed in the C57BL/6-B16 model, Bifidobacterium administration per se has a beneficial effect on CT26 tumor inhibition. Furthermore, the combination of Bifidobacterium administration and vaccination resulted in a protection which was superior to vaccination alone and to Bifidobacterium administration alone, and correlated with an increase in the frequency of vaccine-specific T cells. The gut microbiome analysis by 16S rRNA gene sequencing and shotgun metagenomics showed that tumor challenge rapidly altered the microbiome population, with Muribaculaceae being enriched and Lachnospiraceae being reduced. Over time, the population of Muribaculaceae progressively reduced while the Lachnospiraceae population increased—a trend that appeared to be retarded by the oral administration of Bifidobacterium. Interestingly, in some Bacteroidales, Prevotella and Muribaculacee species we identified sequences highly homologous to immunogenic neo-epitopes of CT26 cells, supporting the possible role of “molecular mimicry” in anticancer immunity. Our data strengthen the importance of the microbiome in cancer immunity and suggests a microbiome-based strategy to potentiate neo-epitope-based cancer vaccines. MDPI 2021-11-18 /pmc/articles/PMC8619961/ /pubmed/34835287 http://dx.doi.org/10.3390/vaccines9111356 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tomasi, Michele
Dalsass, Mattia
Beghini, Francesco
Zanella, Ilaria
Caproni, Elena
Fantappiè, Laura
Gagliardi, Assunta
Irene, Carmela
König, Enrico
Frattini, Luca
Masetti, Giulia
Isaac, Samine Jessica
Armanini, Federica
Cumbo, Fabio
Blanco-Míguez, Aitor
Grandi, Alberto
Segata, Nicola
Grandi, Guido
Commensal Bifidobacterium Strains Enhance the Efficacy of Neo-Epitope Based Cancer Vaccines
title Commensal Bifidobacterium Strains Enhance the Efficacy of Neo-Epitope Based Cancer Vaccines
title_full Commensal Bifidobacterium Strains Enhance the Efficacy of Neo-Epitope Based Cancer Vaccines
title_fullStr Commensal Bifidobacterium Strains Enhance the Efficacy of Neo-Epitope Based Cancer Vaccines
title_full_unstemmed Commensal Bifidobacterium Strains Enhance the Efficacy of Neo-Epitope Based Cancer Vaccines
title_short Commensal Bifidobacterium Strains Enhance the Efficacy of Neo-Epitope Based Cancer Vaccines
title_sort commensal bifidobacterium strains enhance the efficacy of neo-epitope based cancer vaccines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8619961/
https://www.ncbi.nlm.nih.gov/pubmed/34835287
http://dx.doi.org/10.3390/vaccines9111356
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