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Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides

Fusarium graminearum and Fusarium verticillioides are fungal pathogens that cause diseases in cereal crops, such as Fusarium head blight (FHB), seedling blight, and stalk rot. They also produce a variety of mycotoxins that reduce crop yields and threaten human and animal health. Several strategies f...

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Autores principales: Yu, Chenjie, Liu, Xin, Zhang, Xinyue, Zhang, Mengxuan, Gu, Yiying, Ali, Qurban, Mohamed, M. Sherif Ramzy, Xu, Jianhong, Shi, Jianrong, Gao, Xuewen, Wu, Huijun, Gu, Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620035/
https://www.ncbi.nlm.nih.gov/pubmed/34822575
http://dx.doi.org/10.3390/toxins13110791
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author Yu, Chenjie
Liu, Xin
Zhang, Xinyue
Zhang, Mengxuan
Gu, Yiying
Ali, Qurban
Mohamed, M. Sherif Ramzy
Xu, Jianhong
Shi, Jianrong
Gao, Xuewen
Wu, Huijun
Gu, Qin
author_facet Yu, Chenjie
Liu, Xin
Zhang, Xinyue
Zhang, Mengxuan
Gu, Yiying
Ali, Qurban
Mohamed, M. Sherif Ramzy
Xu, Jianhong
Shi, Jianrong
Gao, Xuewen
Wu, Huijun
Gu, Qin
author_sort Yu, Chenjie
collection PubMed
description Fusarium graminearum and Fusarium verticillioides are fungal pathogens that cause diseases in cereal crops, such as Fusarium head blight (FHB), seedling blight, and stalk rot. They also produce a variety of mycotoxins that reduce crop yields and threaten human and animal health. Several strategies for controlling these diseases have been developed. However, due to a lack of resistant cultivars and the hazards of chemical fungicides, efforts are now focused on the biocontrol of plant diseases, which is a more sustainable and environmentally friendly approach. In the present study, the lipopeptide mycosubtilin purified from Bacillus subtilis ATCC6633 significantly suppressed the growth of F. graminearum PH-1 and F. verticillioides 7600 in vitro. Mycosubtilin caused the destruction and deformation of plasma membranes and cell walls in F. graminearum hyphae. Additionally, mycosubtilin inhibited conidial spore formation and germination of both fungi in a dose-dependent manner. In planta experiments demonstrated the ability of mycosubtilin to control the adverse effects caused by F. graminearum and F. verticillioides on wheat heads and maize kernels, respectively. Mycosubtilin significantly decreased the production of deoxynivalenol (DON) and B-series fumonisins (FB(1), FB(2) and FB(3)) in infected grains, with inhibition rates of 48.92, 48.48, 52.42, and 59.44%, respectively. The qRT-PCR analysis showed that mycosubtilin significantly downregulated genes involved in mycotoxin biosynthesis. In conclusion, mycosubtilin produced by B. subtilis ATCC6633 was shown to have potential as a biological agent to control plant diseases and Fusarium toxin contamination caused by F. graminearum and F. verticillioides.
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spelling pubmed-86200352021-11-27 Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides Yu, Chenjie Liu, Xin Zhang, Xinyue Zhang, Mengxuan Gu, Yiying Ali, Qurban Mohamed, M. Sherif Ramzy Xu, Jianhong Shi, Jianrong Gao, Xuewen Wu, Huijun Gu, Qin Toxins (Basel) Article Fusarium graminearum and Fusarium verticillioides are fungal pathogens that cause diseases in cereal crops, such as Fusarium head blight (FHB), seedling blight, and stalk rot. They also produce a variety of mycotoxins that reduce crop yields and threaten human and animal health. Several strategies for controlling these diseases have been developed. However, due to a lack of resistant cultivars and the hazards of chemical fungicides, efforts are now focused on the biocontrol of plant diseases, which is a more sustainable and environmentally friendly approach. In the present study, the lipopeptide mycosubtilin purified from Bacillus subtilis ATCC6633 significantly suppressed the growth of F. graminearum PH-1 and F. verticillioides 7600 in vitro. Mycosubtilin caused the destruction and deformation of plasma membranes and cell walls in F. graminearum hyphae. Additionally, mycosubtilin inhibited conidial spore formation and germination of both fungi in a dose-dependent manner. In planta experiments demonstrated the ability of mycosubtilin to control the adverse effects caused by F. graminearum and F. verticillioides on wheat heads and maize kernels, respectively. Mycosubtilin significantly decreased the production of deoxynivalenol (DON) and B-series fumonisins (FB(1), FB(2) and FB(3)) in infected grains, with inhibition rates of 48.92, 48.48, 52.42, and 59.44%, respectively. The qRT-PCR analysis showed that mycosubtilin significantly downregulated genes involved in mycotoxin biosynthesis. In conclusion, mycosubtilin produced by B. subtilis ATCC6633 was shown to have potential as a biological agent to control plant diseases and Fusarium toxin contamination caused by F. graminearum and F. verticillioides. MDPI 2021-11-09 /pmc/articles/PMC8620035/ /pubmed/34822575 http://dx.doi.org/10.3390/toxins13110791 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Chenjie
Liu, Xin
Zhang, Xinyue
Zhang, Mengxuan
Gu, Yiying
Ali, Qurban
Mohamed, M. Sherif Ramzy
Xu, Jianhong
Shi, Jianrong
Gao, Xuewen
Wu, Huijun
Gu, Qin
Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides
title Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides
title_full Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides
title_fullStr Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides
title_full_unstemmed Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides
title_short Mycosubtilin Produced by Bacillus subtilis ATCC6633 Inhibits Growth and Mycotoxin Biosynthesis of Fusarium graminearum and Fusarium verticillioides
title_sort mycosubtilin produced by bacillus subtilis atcc6633 inhibits growth and mycotoxin biosynthesis of fusarium graminearum and fusarium verticillioides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620035/
https://www.ncbi.nlm.nih.gov/pubmed/34822575
http://dx.doi.org/10.3390/toxins13110791
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