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Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium

Hydroxycarboxylic acid receptor 3 (HCA(3)) was recently identified in the genomes of humans and other hominids but not in other mammals. We examined the production of HCA(3) ligands by Bifidobacterium spp. In addition to 4-hydroxyphenyllactic acid, phenyllactic acid (PLA), and indole-3-lactic acid (...

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Autores principales: Sakurai, Takuma, Horigome, Ayako, Odamaki, Toshitaka, Shimizu, Takashi, Xiao, Jin-Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620054/
https://www.ncbi.nlm.nih.gov/pubmed/34835522
http://dx.doi.org/10.3390/microorganisms9112397
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author Sakurai, Takuma
Horigome, Ayako
Odamaki, Toshitaka
Shimizu, Takashi
Xiao, Jin-Zhong
author_facet Sakurai, Takuma
Horigome, Ayako
Odamaki, Toshitaka
Shimizu, Takashi
Xiao, Jin-Zhong
author_sort Sakurai, Takuma
collection PubMed
description Hydroxycarboxylic acid receptor 3 (HCA(3)) was recently identified in the genomes of humans and other hominids but not in other mammals. We examined the production of HCA(3) ligands by Bifidobacterium spp. In addition to 4-hydroxyphenyllactic acid, phenyllactic acid (PLA), and indole-3-lactic acid (ILA), we found that LeuA was produced by Bifidobacterium as an HCA(3) ligand. The four ligands produced were the mixtures of enantiomers, and D-ILA, D-PLA, and D-LeuA showed stronger activity of the HCA(3) ligand than their respective L-isomers. However, there was no difference in AhR activity between the two ILA enantiomers. These results provide new insights into the HCA(3) ligands produced by Bifidobacterium and suggest the importance of investigating the absolute stereo structures of these metabolites.
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spelling pubmed-86200542021-11-27 Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium Sakurai, Takuma Horigome, Ayako Odamaki, Toshitaka Shimizu, Takashi Xiao, Jin-Zhong Microorganisms Article Hydroxycarboxylic acid receptor 3 (HCA(3)) was recently identified in the genomes of humans and other hominids but not in other mammals. We examined the production of HCA(3) ligands by Bifidobacterium spp. In addition to 4-hydroxyphenyllactic acid, phenyllactic acid (PLA), and indole-3-lactic acid (ILA), we found that LeuA was produced by Bifidobacterium as an HCA(3) ligand. The four ligands produced were the mixtures of enantiomers, and D-ILA, D-PLA, and D-LeuA showed stronger activity of the HCA(3) ligand than their respective L-isomers. However, there was no difference in AhR activity between the two ILA enantiomers. These results provide new insights into the HCA(3) ligands produced by Bifidobacterium and suggest the importance of investigating the absolute stereo structures of these metabolites. MDPI 2021-11-21 /pmc/articles/PMC8620054/ /pubmed/34835522 http://dx.doi.org/10.3390/microorganisms9112397 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sakurai, Takuma
Horigome, Ayako
Odamaki, Toshitaka
Shimizu, Takashi
Xiao, Jin-Zhong
Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium
title Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium
title_full Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium
title_fullStr Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium
title_full_unstemmed Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium
title_short Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium
title_sort production of hydroxycarboxylic acid receptor 3 (hca(3)) ligands by bifidobacterium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620054/
https://www.ncbi.nlm.nih.gov/pubmed/34835522
http://dx.doi.org/10.3390/microorganisms9112397
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