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Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium
Hydroxycarboxylic acid receptor 3 (HCA(3)) was recently identified in the genomes of humans and other hominids but not in other mammals. We examined the production of HCA(3) ligands by Bifidobacterium spp. In addition to 4-hydroxyphenyllactic acid, phenyllactic acid (PLA), and indole-3-lactic acid (...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620054/ https://www.ncbi.nlm.nih.gov/pubmed/34835522 http://dx.doi.org/10.3390/microorganisms9112397 |
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author | Sakurai, Takuma Horigome, Ayako Odamaki, Toshitaka Shimizu, Takashi Xiao, Jin-Zhong |
author_facet | Sakurai, Takuma Horigome, Ayako Odamaki, Toshitaka Shimizu, Takashi Xiao, Jin-Zhong |
author_sort | Sakurai, Takuma |
collection | PubMed |
description | Hydroxycarboxylic acid receptor 3 (HCA(3)) was recently identified in the genomes of humans and other hominids but not in other mammals. We examined the production of HCA(3) ligands by Bifidobacterium spp. In addition to 4-hydroxyphenyllactic acid, phenyllactic acid (PLA), and indole-3-lactic acid (ILA), we found that LeuA was produced by Bifidobacterium as an HCA(3) ligand. The four ligands produced were the mixtures of enantiomers, and D-ILA, D-PLA, and D-LeuA showed stronger activity of the HCA(3) ligand than their respective L-isomers. However, there was no difference in AhR activity between the two ILA enantiomers. These results provide new insights into the HCA(3) ligands produced by Bifidobacterium and suggest the importance of investigating the absolute stereo structures of these metabolites. |
format | Online Article Text |
id | pubmed-8620054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86200542021-11-27 Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium Sakurai, Takuma Horigome, Ayako Odamaki, Toshitaka Shimizu, Takashi Xiao, Jin-Zhong Microorganisms Article Hydroxycarboxylic acid receptor 3 (HCA(3)) was recently identified in the genomes of humans and other hominids but not in other mammals. We examined the production of HCA(3) ligands by Bifidobacterium spp. In addition to 4-hydroxyphenyllactic acid, phenyllactic acid (PLA), and indole-3-lactic acid (ILA), we found that LeuA was produced by Bifidobacterium as an HCA(3) ligand. The four ligands produced were the mixtures of enantiomers, and D-ILA, D-PLA, and D-LeuA showed stronger activity of the HCA(3) ligand than their respective L-isomers. However, there was no difference in AhR activity between the two ILA enantiomers. These results provide new insights into the HCA(3) ligands produced by Bifidobacterium and suggest the importance of investigating the absolute stereo structures of these metabolites. MDPI 2021-11-21 /pmc/articles/PMC8620054/ /pubmed/34835522 http://dx.doi.org/10.3390/microorganisms9112397 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sakurai, Takuma Horigome, Ayako Odamaki, Toshitaka Shimizu, Takashi Xiao, Jin-Zhong Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium |
title | Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium |
title_full | Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium |
title_fullStr | Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium |
title_full_unstemmed | Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium |
title_short | Production of Hydroxycarboxylic Acid Receptor 3 (HCA(3)) Ligands by Bifidobacterium |
title_sort | production of hydroxycarboxylic acid receptor 3 (hca(3)) ligands by bifidobacterium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620054/ https://www.ncbi.nlm.nih.gov/pubmed/34835522 http://dx.doi.org/10.3390/microorganisms9112397 |
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