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Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)
Marine collagen is gaining vast interest because of its high biocompatibility and lack of religious and social restrictions compared with collagen from terrestrial sources. In this study, lizardfish (Synodus macrops) scales were used to isolate acid-soluble collagen (ASC) and pepsin-soluble collagen...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620309/ https://www.ncbi.nlm.nih.gov/pubmed/34822468 http://dx.doi.org/10.3390/md19110597 |
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author | Chen, Junde Wang, Guangyu Li, Yushuang |
author_facet | Chen, Junde Wang, Guangyu Li, Yushuang |
author_sort | Chen, Junde |
collection | PubMed |
description | Marine collagen is gaining vast interest because of its high biocompatibility and lack of religious and social restrictions compared with collagen from terrestrial sources. In this study, lizardfish (Synodus macrops) scales were used to isolate acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC). Both ASC and PSC were identified as type I collagen with intact triple-helix structures by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and spectroscopy. The ASC and PSC had high amino acids of 237 residues/1000 residues and 236 residues/1000 residues, respectively. Thus, the maximum transition temperature (T(max)) of ASC (43.2 °C) was higher than that of PSC (42.5 °C). Interestingly, the T(max) of both ASC and PSC was higher than that of rat tail collagen (39.4 °C) and calf skin collagen (35.0 °C), the terrestrial collagen. Solubility tests showed that both ASC and PSC exhibited high solubility in the acidic pH ranges. ASC was less susceptible to the “salting out” effect compared with PSC. Both collagen types were nontoxic to HaCaT and MC3T3-E1 cells, and ASC was associated with a higher cell viability than PSC. These results indicated that ASC from lizardfish scales could be an alternative to terrestrial sources of collagen, with potential for biomedical applications. |
format | Online Article Text |
id | pubmed-8620309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86203092021-11-27 Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops) Chen, Junde Wang, Guangyu Li, Yushuang Mar Drugs Article Marine collagen is gaining vast interest because of its high biocompatibility and lack of religious and social restrictions compared with collagen from terrestrial sources. In this study, lizardfish (Synodus macrops) scales were used to isolate acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC). Both ASC and PSC were identified as type I collagen with intact triple-helix structures by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and spectroscopy. The ASC and PSC had high amino acids of 237 residues/1000 residues and 236 residues/1000 residues, respectively. Thus, the maximum transition temperature (T(max)) of ASC (43.2 °C) was higher than that of PSC (42.5 °C). Interestingly, the T(max) of both ASC and PSC was higher than that of rat tail collagen (39.4 °C) and calf skin collagen (35.0 °C), the terrestrial collagen. Solubility tests showed that both ASC and PSC exhibited high solubility in the acidic pH ranges. ASC was less susceptible to the “salting out” effect compared with PSC. Both collagen types were nontoxic to HaCaT and MC3T3-E1 cells, and ASC was associated with a higher cell viability than PSC. These results indicated that ASC from lizardfish scales could be an alternative to terrestrial sources of collagen, with potential for biomedical applications. MDPI 2021-10-21 /pmc/articles/PMC8620309/ /pubmed/34822468 http://dx.doi.org/10.3390/md19110597 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Junde Wang, Guangyu Li, Yushuang Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops) |
title | Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops) |
title_full | Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops) |
title_fullStr | Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops) |
title_full_unstemmed | Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops) |
title_short | Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops) |
title_sort | preparation and characterization of thermally stable collagens from the scales of lizardfish (synodus macrops) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620309/ https://www.ncbi.nlm.nih.gov/pubmed/34822468 http://dx.doi.org/10.3390/md19110597 |
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