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Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)

Marine collagen is gaining vast interest because of its high biocompatibility and lack of religious and social restrictions compared with collagen from terrestrial sources. In this study, lizardfish (Synodus macrops) scales were used to isolate acid-soluble collagen (ASC) and pepsin-soluble collagen...

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Autores principales: Chen, Junde, Wang, Guangyu, Li, Yushuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620309/
https://www.ncbi.nlm.nih.gov/pubmed/34822468
http://dx.doi.org/10.3390/md19110597
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author Chen, Junde
Wang, Guangyu
Li, Yushuang
author_facet Chen, Junde
Wang, Guangyu
Li, Yushuang
author_sort Chen, Junde
collection PubMed
description Marine collagen is gaining vast interest because of its high biocompatibility and lack of religious and social restrictions compared with collagen from terrestrial sources. In this study, lizardfish (Synodus macrops) scales were used to isolate acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC). Both ASC and PSC were identified as type I collagen with intact triple-helix structures by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and spectroscopy. The ASC and PSC had high amino acids of 237 residues/1000 residues and 236 residues/1000 residues, respectively. Thus, the maximum transition temperature (T(max)) of ASC (43.2 °C) was higher than that of PSC (42.5 °C). Interestingly, the T(max) of both ASC and PSC was higher than that of rat tail collagen (39.4 °C) and calf skin collagen (35.0 °C), the terrestrial collagen. Solubility tests showed that both ASC and PSC exhibited high solubility in the acidic pH ranges. ASC was less susceptible to the “salting out” effect compared with PSC. Both collagen types were nontoxic to HaCaT and MC3T3-E1 cells, and ASC was associated with a higher cell viability than PSC. These results indicated that ASC from lizardfish scales could be an alternative to terrestrial sources of collagen, with potential for biomedical applications.
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spelling pubmed-86203092021-11-27 Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops) Chen, Junde Wang, Guangyu Li, Yushuang Mar Drugs Article Marine collagen is gaining vast interest because of its high biocompatibility and lack of religious and social restrictions compared with collagen from terrestrial sources. In this study, lizardfish (Synodus macrops) scales were used to isolate acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC). Both ASC and PSC were identified as type I collagen with intact triple-helix structures by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and spectroscopy. The ASC and PSC had high amino acids of 237 residues/1000 residues and 236 residues/1000 residues, respectively. Thus, the maximum transition temperature (T(max)) of ASC (43.2 °C) was higher than that of PSC (42.5 °C). Interestingly, the T(max) of both ASC and PSC was higher than that of rat tail collagen (39.4 °C) and calf skin collagen (35.0 °C), the terrestrial collagen. Solubility tests showed that both ASC and PSC exhibited high solubility in the acidic pH ranges. ASC was less susceptible to the “salting out” effect compared with PSC. Both collagen types were nontoxic to HaCaT and MC3T3-E1 cells, and ASC was associated with a higher cell viability than PSC. These results indicated that ASC from lizardfish scales could be an alternative to terrestrial sources of collagen, with potential for biomedical applications. MDPI 2021-10-21 /pmc/articles/PMC8620309/ /pubmed/34822468 http://dx.doi.org/10.3390/md19110597 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Junde
Wang, Guangyu
Li, Yushuang
Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)
title Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)
title_full Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)
title_fullStr Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)
title_full_unstemmed Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)
title_short Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)
title_sort preparation and characterization of thermally stable collagens from the scales of lizardfish (synodus macrops)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620309/
https://www.ncbi.nlm.nih.gov/pubmed/34822468
http://dx.doi.org/10.3390/md19110597
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