Cargando…

Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress

The present study demonstrates the Ni toxicity-ameliorating and growth-promoting abilities of two different bacterial isolates when applied to wheat (Triticum aestivum L.) as the host plant. Two bacterial strains tolerant to Ni stress were isolated from wheat seeds and selected based on their abilit...

Descripción completa

Detalles Bibliográficos
Autores principales: Pishchik, Veronika N., Filippova, Polina S., Mirskaya, Galina V., Khomyakov, Yuriy V., Vertebny, Vitaliy E., Dubovitskaya, Viktoriya I., Ostankova, Yuliya V., Semenov, Aleksandr V., Chakrabarty, Debasis, Zuev, Evgeny V., Chebotar, Vladimir K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620400/
https://www.ncbi.nlm.nih.gov/pubmed/34834698
http://dx.doi.org/10.3390/plants10112334
_version_ 1784605210974355456
author Pishchik, Veronika N.
Filippova, Polina S.
Mirskaya, Galina V.
Khomyakov, Yuriy V.
Vertebny, Vitaliy E.
Dubovitskaya, Viktoriya I.
Ostankova, Yuliya V.
Semenov, Aleksandr V.
Chakrabarty, Debasis
Zuev, Evgeny V.
Chebotar, Vladimir K.
author_facet Pishchik, Veronika N.
Filippova, Polina S.
Mirskaya, Galina V.
Khomyakov, Yuriy V.
Vertebny, Vitaliy E.
Dubovitskaya, Viktoriya I.
Ostankova, Yuliya V.
Semenov, Aleksandr V.
Chakrabarty, Debasis
Zuev, Evgeny V.
Chebotar, Vladimir K.
author_sort Pishchik, Veronika N.
collection PubMed
description The present study demonstrates the Ni toxicity-ameliorating and growth-promoting abilities of two different bacterial isolates when applied to wheat (Triticum aestivum L.) as the host plant. Two bacterial strains tolerant to Ni stress were isolated from wheat seeds and selected based on their ability to improve the germination of wheat plants; they were identified as Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2. The protective effects of these epiphytic bacteria against Ni stress were studied in model experiments with two wheat cultivars: Ni stress-tolerant Leningradskaya 6 and susceptible Chinese spring. When these isolates were used as the inoculants applied to Ni-treated wheat plants, the growth parameters and the levels of photosynthetic pigments of the two wheat cultivars both under normal and Ni-stress conditions were increased, though B. megaterium AFI1 had a more pronounced ameliorative effect on the Ni contents in plant tissues due to its synthesis of siderophores. Over the 10 days of Ni exposure, the plant growth promotion bacteria (PGPB) significantly reduced the lipid peroxidation (LPO), ascorbate peroxidase (APX), superoxide dismutase (SOD) activities and proline content in the leaves of both wheat cultivars. The PGPB also increased peroxidase (POX) activity and the levels of chlorophyll a, chlorophyll b, and carotenoids in the wheat leaves. It was concluded that B. megaterium AFI1 is an ideal candidate for bioremediation and wheat growth promotion against Ni-induced oxidative stress, as it increases photosynthetic pigment contents, induces the antioxidant defense system, and lowers Ni metal uptake.
format Online
Article
Text
id pubmed-8620400
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86204002021-11-27 Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress Pishchik, Veronika N. Filippova, Polina S. Mirskaya, Galina V. Khomyakov, Yuriy V. Vertebny, Vitaliy E. Dubovitskaya, Viktoriya I. Ostankova, Yuliya V. Semenov, Aleksandr V. Chakrabarty, Debasis Zuev, Evgeny V. Chebotar, Vladimir K. Plants (Basel) Article The present study demonstrates the Ni toxicity-ameliorating and growth-promoting abilities of two different bacterial isolates when applied to wheat (Triticum aestivum L.) as the host plant. Two bacterial strains tolerant to Ni stress were isolated from wheat seeds and selected based on their ability to improve the germination of wheat plants; they were identified as Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2. The protective effects of these epiphytic bacteria against Ni stress were studied in model experiments with two wheat cultivars: Ni stress-tolerant Leningradskaya 6 and susceptible Chinese spring. When these isolates were used as the inoculants applied to Ni-treated wheat plants, the growth parameters and the levels of photosynthetic pigments of the two wheat cultivars both under normal and Ni-stress conditions were increased, though B. megaterium AFI1 had a more pronounced ameliorative effect on the Ni contents in plant tissues due to its synthesis of siderophores. Over the 10 days of Ni exposure, the plant growth promotion bacteria (PGPB) significantly reduced the lipid peroxidation (LPO), ascorbate peroxidase (APX), superoxide dismutase (SOD) activities and proline content in the leaves of both wheat cultivars. The PGPB also increased peroxidase (POX) activity and the levels of chlorophyll a, chlorophyll b, and carotenoids in the wheat leaves. It was concluded that B. megaterium AFI1 is an ideal candidate for bioremediation and wheat growth promotion against Ni-induced oxidative stress, as it increases photosynthetic pigment contents, induces the antioxidant defense system, and lowers Ni metal uptake. MDPI 2021-10-29 /pmc/articles/PMC8620400/ /pubmed/34834698 http://dx.doi.org/10.3390/plants10112334 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pishchik, Veronika N.
Filippova, Polina S.
Mirskaya, Galina V.
Khomyakov, Yuriy V.
Vertebny, Vitaliy E.
Dubovitskaya, Viktoriya I.
Ostankova, Yuliya V.
Semenov, Aleksandr V.
Chakrabarty, Debasis
Zuev, Evgeny V.
Chebotar, Vladimir K.
Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_full Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_fullStr Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_full_unstemmed Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_short Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_sort epiphytic pgpb bacillus megaterium afi1 and paenibacillus nicotianae afi2 improve wheat growth and antioxidant status under ni stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620400/
https://www.ncbi.nlm.nih.gov/pubmed/34834698
http://dx.doi.org/10.3390/plants10112334
work_keys_str_mv AT pishchikveronikan epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT filippovapolinas epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT mirskayagalinav epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT khomyakovyuriyv epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT vertebnyvitaliye epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT dubovitskayaviktoriyai epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT ostankovayuliyav epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT semenovaleksandrv epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT chakrabartydebasis epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT zuevevgenyv epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress
AT chebotarvladimirk epiphyticpgpbbacillusmegateriumafi1andpaenibacillusnicotianaeafi2improvewheatgrowthandantioxidantstatusundernistress