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Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells

Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including the master regulator Twist...

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Autores principales: Pires, Bruno R. B., Binato, Renata, Ferreira, Gerson M., Corrêa, Stephany, Du Rocher, Bárbara, Bulzico, Daniel, Crocamo, Susanne, dos Santos, Everton Cruz, Lima, Luize G., Abdelhay, Eliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620489/
https://www.ncbi.nlm.nih.gov/pubmed/34830027
http://dx.doi.org/10.3390/ijms222212144
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author Pires, Bruno R. B.
Binato, Renata
Ferreira, Gerson M.
Corrêa, Stephany
Du Rocher, Bárbara
Bulzico, Daniel
Crocamo, Susanne
dos Santos, Everton Cruz
Lima, Luize G.
Abdelhay, Eliana
author_facet Pires, Bruno R. B.
Binato, Renata
Ferreira, Gerson M.
Corrêa, Stephany
Du Rocher, Bárbara
Bulzico, Daniel
Crocamo, Susanne
dos Santos, Everton Cruz
Lima, Luize G.
Abdelhay, Eliana
author_sort Pires, Bruno R. B.
collection PubMed
description Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including the master regulator Twist1. However, its role beyond EMT in BC subtypes remains unclear. Our study aimed to investigate the role of Twist1, beyond EMT, in the molecular subtypes of BC. In patients, we observed the overexpression of TWIST1 in the HER2+ group. The silencing of TWIST1 in HER2+ BC cells resulted in the upregulation of 138 genes and the downregulation of 174 genes compared to control cells in a microarray assay. In silico analysis revealed correlations between Twist1 and important biological processes such as the Th17-mediated immune response, suggesting that Twist1 could be relevant for IL-17 signaling in HER2+ BC. IL-17 signaling was then examined, and it was shown that TWIST1 knockdown caused the downregulation of leading members of IL-17 signaling pathway. Taken together, our findings suggest that Twist1 plays a role on IL-17 signaling in HER2+ BC.
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spelling pubmed-86204892021-11-27 Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells Pires, Bruno R. B. Binato, Renata Ferreira, Gerson M. Corrêa, Stephany Du Rocher, Bárbara Bulzico, Daniel Crocamo, Susanne dos Santos, Everton Cruz Lima, Luize G. Abdelhay, Eliana Int J Mol Sci Article Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including the master regulator Twist1. However, its role beyond EMT in BC subtypes remains unclear. Our study aimed to investigate the role of Twist1, beyond EMT, in the molecular subtypes of BC. In patients, we observed the overexpression of TWIST1 in the HER2+ group. The silencing of TWIST1 in HER2+ BC cells resulted in the upregulation of 138 genes and the downregulation of 174 genes compared to control cells in a microarray assay. In silico analysis revealed correlations between Twist1 and important biological processes such as the Th17-mediated immune response, suggesting that Twist1 could be relevant for IL-17 signaling in HER2+ BC. IL-17 signaling was then examined, and it was shown that TWIST1 knockdown caused the downregulation of leading members of IL-17 signaling pathway. Taken together, our findings suggest that Twist1 plays a role on IL-17 signaling in HER2+ BC. MDPI 2021-11-10 /pmc/articles/PMC8620489/ /pubmed/34830027 http://dx.doi.org/10.3390/ijms222212144 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pires, Bruno R. B.
Binato, Renata
Ferreira, Gerson M.
Corrêa, Stephany
Du Rocher, Bárbara
Bulzico, Daniel
Crocamo, Susanne
dos Santos, Everton Cruz
Lima, Luize G.
Abdelhay, Eliana
Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_full Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_fullStr Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_full_unstemmed Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_short Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_sort twist1 influences the expression of leading members of the il-17 signaling pathway in her2-positive breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620489/
https://www.ncbi.nlm.nih.gov/pubmed/34830027
http://dx.doi.org/10.3390/ijms222212144
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