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Short-term storage of semen samples in acidic extender increases the proportion of females in pigs

BACKGROUND: Sex preselection is a desired goal of the animal industry to improve production efficiency, depending on industry demand. In the porcine industry, there is a general preference for pork from female and surgically castrated male pigs. Therefore, the birth of more females than males in a l...

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Autores principales: Park, Yoo-Jin, Shin, Dong-Ha, Pang, Won-Ki, Ryu, Do-Yeal, Rahman, Md Saidur, Adegoke, Elikanah Olusayo, Pang, Myung-Geol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620557/
https://www.ncbi.nlm.nih.gov/pubmed/34836535
http://dx.doi.org/10.1186/s12917-021-03078-3
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author Park, Yoo-Jin
Shin, Dong-Ha
Pang, Won-Ki
Ryu, Do-Yeal
Rahman, Md Saidur
Adegoke, Elikanah Olusayo
Pang, Myung-Geol
author_facet Park, Yoo-Jin
Shin, Dong-Ha
Pang, Won-Ki
Ryu, Do-Yeal
Rahman, Md Saidur
Adegoke, Elikanah Olusayo
Pang, Myung-Geol
author_sort Park, Yoo-Jin
collection PubMed
description BACKGROUND: Sex preselection is a desired goal of the animal industry to improve production efficiency, depending on industry demand. In the porcine industry, there is a general preference for pork from female and surgically castrated male pigs. Therefore, the birth of more females than males in a litter leads to economic benefits and improved animal welfare in the pig production industry. Our previous study suggested that the porcine semen extender (BTS) adjusted to pH 6.2 maximises the differences in viability between X-chromosome-bearing (X) spermatozoa and Y-chromosome-bearing (Y) spermatozoa without affecting sperm’s functional parameters. In this study we aimed to evaluate whether the pH 6.2 extender is applicable at the farm level for increasing the number of female piglets without a decline in spermatozoa fertility. Artificial insemination (AI) was carried out with spermatozoa stored at pH 6.2 and pH 7.2 (original BTS) at day 1 and day 2 of storage. Next, the functional parameters of the spermatozoa, litter size, farrowing rate, and female-to-male ratio of offspring were determined. RESULTS: Although sperm motility decreased significantly after 2 d of storage, the viability of spermatozoa was preserved at pH 6.2 for 3 d. There was no significant difference in the farrowing rate and average litter size between the group inseminated with the spermatozoa stored in (pH 7.2) and that inseminated with spermatozoa stored in acidic BTS. The percentage of female piglets was approximately 1.5-fold higher in sows inseminated on day 1 in the pH 6.2 than in the pH 7.2 group. Furthermore, although there was no significant difference in the female-to-male ratio, the percentage of female piglets born was slightly higher in the pH 6.2 group than in the pH 7.2 group on day 2. CONCLUSIONS: The method optimised in our study is simple, economical, and may enhance the number of female births without any decline in spermatozoa fertility.
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spelling pubmed-86205572021-11-29 Short-term storage of semen samples in acidic extender increases the proportion of females in pigs Park, Yoo-Jin Shin, Dong-Ha Pang, Won-Ki Ryu, Do-Yeal Rahman, Md Saidur Adegoke, Elikanah Olusayo Pang, Myung-Geol BMC Vet Res Research BACKGROUND: Sex preselection is a desired goal of the animal industry to improve production efficiency, depending on industry demand. In the porcine industry, there is a general preference for pork from female and surgically castrated male pigs. Therefore, the birth of more females than males in a litter leads to economic benefits and improved animal welfare in the pig production industry. Our previous study suggested that the porcine semen extender (BTS) adjusted to pH 6.2 maximises the differences in viability between X-chromosome-bearing (X) spermatozoa and Y-chromosome-bearing (Y) spermatozoa without affecting sperm’s functional parameters. In this study we aimed to evaluate whether the pH 6.2 extender is applicable at the farm level for increasing the number of female piglets without a decline in spermatozoa fertility. Artificial insemination (AI) was carried out with spermatozoa stored at pH 6.2 and pH 7.2 (original BTS) at day 1 and day 2 of storage. Next, the functional parameters of the spermatozoa, litter size, farrowing rate, and female-to-male ratio of offspring were determined. RESULTS: Although sperm motility decreased significantly after 2 d of storage, the viability of spermatozoa was preserved at pH 6.2 for 3 d. There was no significant difference in the farrowing rate and average litter size between the group inseminated with the spermatozoa stored in (pH 7.2) and that inseminated with spermatozoa stored in acidic BTS. The percentage of female piglets was approximately 1.5-fold higher in sows inseminated on day 1 in the pH 6.2 than in the pH 7.2 group. Furthermore, although there was no significant difference in the female-to-male ratio, the percentage of female piglets born was slightly higher in the pH 6.2 group than in the pH 7.2 group on day 2. CONCLUSIONS: The method optimised in our study is simple, economical, and may enhance the number of female births without any decline in spermatozoa fertility. BioMed Central 2021-11-26 /pmc/articles/PMC8620557/ /pubmed/34836535 http://dx.doi.org/10.1186/s12917-021-03078-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Park, Yoo-Jin
Shin, Dong-Ha
Pang, Won-Ki
Ryu, Do-Yeal
Rahman, Md Saidur
Adegoke, Elikanah Olusayo
Pang, Myung-Geol
Short-term storage of semen samples in acidic extender increases the proportion of females in pigs
title Short-term storage of semen samples in acidic extender increases the proportion of females in pigs
title_full Short-term storage of semen samples in acidic extender increases the proportion of females in pigs
title_fullStr Short-term storage of semen samples in acidic extender increases the proportion of females in pigs
title_full_unstemmed Short-term storage of semen samples in acidic extender increases the proportion of females in pigs
title_short Short-term storage of semen samples in acidic extender increases the proportion of females in pigs
title_sort short-term storage of semen samples in acidic extender increases the proportion of females in pigs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620557/
https://www.ncbi.nlm.nih.gov/pubmed/34836535
http://dx.doi.org/10.1186/s12917-021-03078-3
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