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Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish

Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterizat...

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Autores principales: Raby, Ludivine, Völkel, Pamela, Hasanpour, Shaghayegh, Cicero, Julien, Toillon, Robert-Alain, Adriaenssens, Eric, Van Seuningen, Isabelle, Le Bourhis, Xuefen, Angrand, Pierre-Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620594/
https://www.ncbi.nlm.nih.gov/pubmed/34831364
http://dx.doi.org/10.3390/cells10113142
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author Raby, Ludivine
Völkel, Pamela
Hasanpour, Shaghayegh
Cicero, Julien
Toillon, Robert-Alain
Adriaenssens, Eric
Van Seuningen, Isabelle
Le Bourhis, Xuefen
Angrand, Pierre-Olivier
author_facet Raby, Ludivine
Völkel, Pamela
Hasanpour, Shaghayegh
Cicero, Julien
Toillon, Robert-Alain
Adriaenssens, Eric
Van Seuningen, Isabelle
Le Bourhis, Xuefen
Angrand, Pierre-Olivier
author_sort Raby, Ludivine
collection PubMed
description Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterization of a zebrafish line harboring a null allele of eed, a gene coding for an essential component of the PRC2. Homozygous eed-deficient mutants present a normal body plan development but display strong defects at the level of the digestive organs, such as reduced size of the pancreas, hepatic steatosis, and a loss of the intestinal structures, to die finally at around 10–12 days post fertilization. In addition, we found that PRC2 loss of function impairs neuronal differentiation in very specific and discrete areas of the brain and increases larval activity in locomotor assays. Our work highlights that zebrafish is a suited model to study human pathologies associated with PRC2 loss of function and H3K27me3 decrease.
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spelling pubmed-86205942021-11-27 Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish Raby, Ludivine Völkel, Pamela Hasanpour, Shaghayegh Cicero, Julien Toillon, Robert-Alain Adriaenssens, Eric Van Seuningen, Isabelle Le Bourhis, Xuefen Angrand, Pierre-Olivier Cells Article Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterization of a zebrafish line harboring a null allele of eed, a gene coding for an essential component of the PRC2. Homozygous eed-deficient mutants present a normal body plan development but display strong defects at the level of the digestive organs, such as reduced size of the pancreas, hepatic steatosis, and a loss of the intestinal structures, to die finally at around 10–12 days post fertilization. In addition, we found that PRC2 loss of function impairs neuronal differentiation in very specific and discrete areas of the brain and increases larval activity in locomotor assays. Our work highlights that zebrafish is a suited model to study human pathologies associated with PRC2 loss of function and H3K27me3 decrease. MDPI 2021-11-12 /pmc/articles/PMC8620594/ /pubmed/34831364 http://dx.doi.org/10.3390/cells10113142 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raby, Ludivine
Völkel, Pamela
Hasanpour, Shaghayegh
Cicero, Julien
Toillon, Robert-Alain
Adriaenssens, Eric
Van Seuningen, Isabelle
Le Bourhis, Xuefen
Angrand, Pierre-Olivier
Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_full Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_fullStr Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_full_unstemmed Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_short Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
title_sort loss of polycomb repressive complex 2 function alters digestive organ homeostasis and neuronal differentiation in zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620594/
https://www.ncbi.nlm.nih.gov/pubmed/34831364
http://dx.doi.org/10.3390/cells10113142
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