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Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterizat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620594/ https://www.ncbi.nlm.nih.gov/pubmed/34831364 http://dx.doi.org/10.3390/cells10113142 |
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author | Raby, Ludivine Völkel, Pamela Hasanpour, Shaghayegh Cicero, Julien Toillon, Robert-Alain Adriaenssens, Eric Van Seuningen, Isabelle Le Bourhis, Xuefen Angrand, Pierre-Olivier |
author_facet | Raby, Ludivine Völkel, Pamela Hasanpour, Shaghayegh Cicero, Julien Toillon, Robert-Alain Adriaenssens, Eric Van Seuningen, Isabelle Le Bourhis, Xuefen Angrand, Pierre-Olivier |
author_sort | Raby, Ludivine |
collection | PubMed |
description | Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterization of a zebrafish line harboring a null allele of eed, a gene coding for an essential component of the PRC2. Homozygous eed-deficient mutants present a normal body plan development but display strong defects at the level of the digestive organs, such as reduced size of the pancreas, hepatic steatosis, and a loss of the intestinal structures, to die finally at around 10–12 days post fertilization. In addition, we found that PRC2 loss of function impairs neuronal differentiation in very specific and discrete areas of the brain and increases larval activity in locomotor assays. Our work highlights that zebrafish is a suited model to study human pathologies associated with PRC2 loss of function and H3K27me3 decrease. |
format | Online Article Text |
id | pubmed-8620594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86205942021-11-27 Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish Raby, Ludivine Völkel, Pamela Hasanpour, Shaghayegh Cicero, Julien Toillon, Robert-Alain Adriaenssens, Eric Van Seuningen, Isabelle Le Bourhis, Xuefen Angrand, Pierre-Olivier Cells Article Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterization of a zebrafish line harboring a null allele of eed, a gene coding for an essential component of the PRC2. Homozygous eed-deficient mutants present a normal body plan development but display strong defects at the level of the digestive organs, such as reduced size of the pancreas, hepatic steatosis, and a loss of the intestinal structures, to die finally at around 10–12 days post fertilization. In addition, we found that PRC2 loss of function impairs neuronal differentiation in very specific and discrete areas of the brain and increases larval activity in locomotor assays. Our work highlights that zebrafish is a suited model to study human pathologies associated with PRC2 loss of function and H3K27me3 decrease. MDPI 2021-11-12 /pmc/articles/PMC8620594/ /pubmed/34831364 http://dx.doi.org/10.3390/cells10113142 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Raby, Ludivine Völkel, Pamela Hasanpour, Shaghayegh Cicero, Julien Toillon, Robert-Alain Adriaenssens, Eric Van Seuningen, Isabelle Le Bourhis, Xuefen Angrand, Pierre-Olivier Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish |
title | Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish |
title_full | Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish |
title_fullStr | Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish |
title_full_unstemmed | Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish |
title_short | Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish |
title_sort | loss of polycomb repressive complex 2 function alters digestive organ homeostasis and neuronal differentiation in zebrafish |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620594/ https://www.ncbi.nlm.nih.gov/pubmed/34831364 http://dx.doi.org/10.3390/cells10113142 |
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