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Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats
The effect of unmodified and functionalized Spirulina platensis biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning el...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620753/ https://www.ncbi.nlm.nih.gov/pubmed/34835756 http://dx.doi.org/10.3390/nano11112992 |
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author | Rudi, Ludmila Zinicovscaia, Inga Cepoi, Liliana Chiriac, Tatiana Peshkova, Alexandra Cepoi, Anastasia Grozdov, Dmitrii |
author_facet | Rudi, Ludmila Zinicovscaia, Inga Cepoi, Liliana Chiriac, Tatiana Peshkova, Alexandra Cepoi, Anastasia Grozdov, Dmitrii |
author_sort | Rudi, Ludmila |
collection | PubMed |
description | The effect of unmodified and functionalized Spirulina platensis biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning electron microscopy and energy dispersive analysis. The content of silver in the different organs of rats after a period of administration (28 days) or after an additional clearance period (28 days) was ascertained by using neutron activation analysis. In animals administrated with the unmodified nanoparticles, the highest content of silver was determined in the brain and kidneys, while in animals administrated with AgNP-Spirulina, silver was mainly accumulated in the brain and testicles. After the clearance period, silver was excreted rapidly from the spleen and kidneys; however, the excretion from the brain was very low, regardless of the type of nanoparticles. Hematological and biochemical tests were performed in order to reveal the effect of nanoparticles on rats. The difference in the content of eosinophils in the experimental and control groups was statistically significant. The hematological indices of the rats did not change significantly under the action of the silver nanoparticles except for the content of reticulocytes and eosinophils, which increased significantly. Changes in the biochemical parameters did not exceed the limits of normal values. Silver nanoparticles with the sizes of 8–20 nm can penetrate the blood–brain barrier, and their persistence after a period of clearance indicated the irreversibility of this process. |
format | Online Article Text |
id | pubmed-8620753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86207532021-11-27 Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats Rudi, Ludmila Zinicovscaia, Inga Cepoi, Liliana Chiriac, Tatiana Peshkova, Alexandra Cepoi, Anastasia Grozdov, Dmitrii Nanomaterials (Basel) Article The effect of unmodified and functionalized Spirulina platensis biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning electron microscopy and energy dispersive analysis. The content of silver in the different organs of rats after a period of administration (28 days) or after an additional clearance period (28 days) was ascertained by using neutron activation analysis. In animals administrated with the unmodified nanoparticles, the highest content of silver was determined in the brain and kidneys, while in animals administrated with AgNP-Spirulina, silver was mainly accumulated in the brain and testicles. After the clearance period, silver was excreted rapidly from the spleen and kidneys; however, the excretion from the brain was very low, regardless of the type of nanoparticles. Hematological and biochemical tests were performed in order to reveal the effect of nanoparticles on rats. The difference in the content of eosinophils in the experimental and control groups was statistically significant. The hematological indices of the rats did not change significantly under the action of the silver nanoparticles except for the content of reticulocytes and eosinophils, which increased significantly. Changes in the biochemical parameters did not exceed the limits of normal values. Silver nanoparticles with the sizes of 8–20 nm can penetrate the blood–brain barrier, and their persistence after a period of clearance indicated the irreversibility of this process. MDPI 2021-11-07 /pmc/articles/PMC8620753/ /pubmed/34835756 http://dx.doi.org/10.3390/nano11112992 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rudi, Ludmila Zinicovscaia, Inga Cepoi, Liliana Chiriac, Tatiana Peshkova, Alexandra Cepoi, Anastasia Grozdov, Dmitrii Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats |
title | Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats |
title_full | Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats |
title_fullStr | Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats |
title_full_unstemmed | Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats |
title_short | Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats |
title_sort | accumulation and effect of silver nanoparticles functionalized with spirulina platensis on rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620753/ https://www.ncbi.nlm.nih.gov/pubmed/34835756 http://dx.doi.org/10.3390/nano11112992 |
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