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Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats

The effect of unmodified and functionalized Spirulina platensis biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning el...

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Autores principales: Rudi, Ludmila, Zinicovscaia, Inga, Cepoi, Liliana, Chiriac, Tatiana, Peshkova, Alexandra, Cepoi, Anastasia, Grozdov, Dmitrii
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620753/
https://www.ncbi.nlm.nih.gov/pubmed/34835756
http://dx.doi.org/10.3390/nano11112992
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author Rudi, Ludmila
Zinicovscaia, Inga
Cepoi, Liliana
Chiriac, Tatiana
Peshkova, Alexandra
Cepoi, Anastasia
Grozdov, Dmitrii
author_facet Rudi, Ludmila
Zinicovscaia, Inga
Cepoi, Liliana
Chiriac, Tatiana
Peshkova, Alexandra
Cepoi, Anastasia
Grozdov, Dmitrii
author_sort Rudi, Ludmila
collection PubMed
description The effect of unmodified and functionalized Spirulina platensis biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning electron microscopy and energy dispersive analysis. The content of silver in the different organs of rats after a period of administration (28 days) or after an additional clearance period (28 days) was ascertained by using neutron activation analysis. In animals administrated with the unmodified nanoparticles, the highest content of silver was determined in the brain and kidneys, while in animals administrated with AgNP-Spirulina, silver was mainly accumulated in the brain and testicles. After the clearance period, silver was excreted rapidly from the spleen and kidneys; however, the excretion from the brain was very low, regardless of the type of nanoparticles. Hematological and biochemical tests were performed in order to reveal the effect of nanoparticles on rats. The difference in the content of eosinophils in the experimental and control groups was statistically significant. The hematological indices of the rats did not change significantly under the action of the silver nanoparticles except for the content of reticulocytes and eosinophils, which increased significantly. Changes in the biochemical parameters did not exceed the limits of normal values. Silver nanoparticles with the sizes of 8–20 nm can penetrate the blood–brain barrier, and their persistence after a period of clearance indicated the irreversibility of this process.
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spelling pubmed-86207532021-11-27 Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats Rudi, Ludmila Zinicovscaia, Inga Cepoi, Liliana Chiriac, Tatiana Peshkova, Alexandra Cepoi, Anastasia Grozdov, Dmitrii Nanomaterials (Basel) Article The effect of unmodified and functionalized Spirulina platensis biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning electron microscopy and energy dispersive analysis. The content of silver in the different organs of rats after a period of administration (28 days) or after an additional clearance period (28 days) was ascertained by using neutron activation analysis. In animals administrated with the unmodified nanoparticles, the highest content of silver was determined in the brain and kidneys, while in animals administrated with AgNP-Spirulina, silver was mainly accumulated in the brain and testicles. After the clearance period, silver was excreted rapidly from the spleen and kidneys; however, the excretion from the brain was very low, regardless of the type of nanoparticles. Hematological and biochemical tests were performed in order to reveal the effect of nanoparticles on rats. The difference in the content of eosinophils in the experimental and control groups was statistically significant. The hematological indices of the rats did not change significantly under the action of the silver nanoparticles except for the content of reticulocytes and eosinophils, which increased significantly. Changes in the biochemical parameters did not exceed the limits of normal values. Silver nanoparticles with the sizes of 8–20 nm can penetrate the blood–brain barrier, and their persistence after a period of clearance indicated the irreversibility of this process. MDPI 2021-11-07 /pmc/articles/PMC8620753/ /pubmed/34835756 http://dx.doi.org/10.3390/nano11112992 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rudi, Ludmila
Zinicovscaia, Inga
Cepoi, Liliana
Chiriac, Tatiana
Peshkova, Alexandra
Cepoi, Anastasia
Grozdov, Dmitrii
Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats
title Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats
title_full Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats
title_fullStr Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats
title_full_unstemmed Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats
title_short Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats
title_sort accumulation and effect of silver nanoparticles functionalized with spirulina platensis on rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620753/
https://www.ncbi.nlm.nih.gov/pubmed/34835756
http://dx.doi.org/10.3390/nano11112992
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