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Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana
A range of basidiomycetes including the edible mushroom Pleurotus ostreatus (Po) can suppress plant pathogens such as Fusarium spp. With the current increase in production and consumption of Po in Uganda, the spent Po substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB),...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620842/ https://www.ncbi.nlm.nih.gov/pubmed/34829233 http://dx.doi.org/10.3390/jof7110946 |
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author | Ocimati, Walter Were, Evans Tazuba, Anthony Fredrick Dita, Miguel Zheng, Si-Jun Blomme, Guy |
author_facet | Ocimati, Walter Were, Evans Tazuba, Anthony Fredrick Dita, Miguel Zheng, Si-Jun Blomme, Guy |
author_sort | Ocimati, Walter |
collection | PubMed |
description | A range of basidiomycetes including the edible mushroom Pleurotus ostreatus (Po) can suppress plant pathogens such as Fusarium spp. With the current increase in production and consumption of Po in Uganda, the spent Po substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB), caused by the soil borne pathogen Fusarium oxysporum f. sp. cubense, race 1 (Foc). This study determined the potential of SPoS to inhibit Foc in vitro and in potted plants. In vitro studies confirmed suppression of Foc in pure co-culture (Po vs. Foc) assays and media amended with different concentrations (0% to 50% w/v) of un-sterilized SPoS filtrates. Foc growth in the sterile SPoS filtrate was comparable to the water control, suggesting possible roles of biotic or thermolabile components of the SPoS. To further verify the suppressive effects of SPoS, pot experiments were carried out with a resistant (‘Mbwazirume’, AAA) and susceptible (‘Sukali Ndizi’, AAB) banana cultivar using both artificially and naturally infested soils. Independent of the inoculation method, SPoS significantly reduced the severity of FWB in pot experiments. Susceptible cultivar ‘Sukali Ndizi’ growing in substrates amended with SPoS showed lower (1.25) corm damage (Scale 0–5) than the un-amended control (3.75). No corm damage was observed in uninoculated controls. The resistant cultivar ‘Mbwazirume’, showed slight (0.25) corm damage only in the Foc-inoculated plants without SPoS. These findings suggest that SPoS could be used as part of the management practices to reduce the impact of FWB. |
format | Online Article Text |
id | pubmed-8620842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86208422021-11-27 Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana Ocimati, Walter Were, Evans Tazuba, Anthony Fredrick Dita, Miguel Zheng, Si-Jun Blomme, Guy J Fungi (Basel) Article A range of basidiomycetes including the edible mushroom Pleurotus ostreatus (Po) can suppress plant pathogens such as Fusarium spp. With the current increase in production and consumption of Po in Uganda, the spent Po substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB), caused by the soil borne pathogen Fusarium oxysporum f. sp. cubense, race 1 (Foc). This study determined the potential of SPoS to inhibit Foc in vitro and in potted plants. In vitro studies confirmed suppression of Foc in pure co-culture (Po vs. Foc) assays and media amended with different concentrations (0% to 50% w/v) of un-sterilized SPoS filtrates. Foc growth in the sterile SPoS filtrate was comparable to the water control, suggesting possible roles of biotic or thermolabile components of the SPoS. To further verify the suppressive effects of SPoS, pot experiments were carried out with a resistant (‘Mbwazirume’, AAA) and susceptible (‘Sukali Ndizi’, AAB) banana cultivar using both artificially and naturally infested soils. Independent of the inoculation method, SPoS significantly reduced the severity of FWB in pot experiments. Susceptible cultivar ‘Sukali Ndizi’ growing in substrates amended with SPoS showed lower (1.25) corm damage (Scale 0–5) than the un-amended control (3.75). No corm damage was observed in uninoculated controls. The resistant cultivar ‘Mbwazirume’, showed slight (0.25) corm damage only in the Foc-inoculated plants without SPoS. These findings suggest that SPoS could be used as part of the management practices to reduce the impact of FWB. MDPI 2021-11-09 /pmc/articles/PMC8620842/ /pubmed/34829233 http://dx.doi.org/10.3390/jof7110946 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ocimati, Walter Were, Evans Tazuba, Anthony Fredrick Dita, Miguel Zheng, Si-Jun Blomme, Guy Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana |
title | Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana |
title_full | Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana |
title_fullStr | Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana |
title_full_unstemmed | Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana |
title_short | Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana |
title_sort | spent pleurotus ostreatus substrate has potential for managing fusarium wilt of banana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620842/ https://www.ncbi.nlm.nih.gov/pubmed/34829233 http://dx.doi.org/10.3390/jof7110946 |
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