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Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana

A range of basidiomycetes including the edible mushroom Pleurotus ostreatus (Po) can suppress plant pathogens such as Fusarium spp. With the current increase in production and consumption of Po in Uganda, the spent Po substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB),...

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Autores principales: Ocimati, Walter, Were, Evans, Tazuba, Anthony Fredrick, Dita, Miguel, Zheng, Si-Jun, Blomme, Guy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620842/
https://www.ncbi.nlm.nih.gov/pubmed/34829233
http://dx.doi.org/10.3390/jof7110946
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author Ocimati, Walter
Were, Evans
Tazuba, Anthony Fredrick
Dita, Miguel
Zheng, Si-Jun
Blomme, Guy
author_facet Ocimati, Walter
Were, Evans
Tazuba, Anthony Fredrick
Dita, Miguel
Zheng, Si-Jun
Blomme, Guy
author_sort Ocimati, Walter
collection PubMed
description A range of basidiomycetes including the edible mushroom Pleurotus ostreatus (Po) can suppress plant pathogens such as Fusarium spp. With the current increase in production and consumption of Po in Uganda, the spent Po substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB), caused by the soil borne pathogen Fusarium oxysporum f. sp. cubense, race 1 (Foc). This study determined the potential of SPoS to inhibit Foc in vitro and in potted plants. In vitro studies confirmed suppression of Foc in pure co-culture (Po vs. Foc) assays and media amended with different concentrations (0% to 50% w/v) of un-sterilized SPoS filtrates. Foc growth in the sterile SPoS filtrate was comparable to the water control, suggesting possible roles of biotic or thermolabile components of the SPoS. To further verify the suppressive effects of SPoS, pot experiments were carried out with a resistant (‘Mbwazirume’, AAA) and susceptible (‘Sukali Ndizi’, AAB) banana cultivar using both artificially and naturally infested soils. Independent of the inoculation method, SPoS significantly reduced the severity of FWB in pot experiments. Susceptible cultivar ‘Sukali Ndizi’ growing in substrates amended with SPoS showed lower (1.25) corm damage (Scale 0–5) than the un-amended control (3.75). No corm damage was observed in uninoculated controls. The resistant cultivar ‘Mbwazirume’, showed slight (0.25) corm damage only in the Foc-inoculated plants without SPoS. These findings suggest that SPoS could be used as part of the management practices to reduce the impact of FWB.
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spelling pubmed-86208422021-11-27 Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana Ocimati, Walter Were, Evans Tazuba, Anthony Fredrick Dita, Miguel Zheng, Si-Jun Blomme, Guy J Fungi (Basel) Article A range of basidiomycetes including the edible mushroom Pleurotus ostreatus (Po) can suppress plant pathogens such as Fusarium spp. With the current increase in production and consumption of Po in Uganda, the spent Po substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB), caused by the soil borne pathogen Fusarium oxysporum f. sp. cubense, race 1 (Foc). This study determined the potential of SPoS to inhibit Foc in vitro and in potted plants. In vitro studies confirmed suppression of Foc in pure co-culture (Po vs. Foc) assays and media amended with different concentrations (0% to 50% w/v) of un-sterilized SPoS filtrates. Foc growth in the sterile SPoS filtrate was comparable to the water control, suggesting possible roles of biotic or thermolabile components of the SPoS. To further verify the suppressive effects of SPoS, pot experiments were carried out with a resistant (‘Mbwazirume’, AAA) and susceptible (‘Sukali Ndizi’, AAB) banana cultivar using both artificially and naturally infested soils. Independent of the inoculation method, SPoS significantly reduced the severity of FWB in pot experiments. Susceptible cultivar ‘Sukali Ndizi’ growing in substrates amended with SPoS showed lower (1.25) corm damage (Scale 0–5) than the un-amended control (3.75). No corm damage was observed in uninoculated controls. The resistant cultivar ‘Mbwazirume’, showed slight (0.25) corm damage only in the Foc-inoculated plants without SPoS. These findings suggest that SPoS could be used as part of the management practices to reduce the impact of FWB. MDPI 2021-11-09 /pmc/articles/PMC8620842/ /pubmed/34829233 http://dx.doi.org/10.3390/jof7110946 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ocimati, Walter
Were, Evans
Tazuba, Anthony Fredrick
Dita, Miguel
Zheng, Si-Jun
Blomme, Guy
Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana
title Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana
title_full Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana
title_fullStr Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana
title_full_unstemmed Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana
title_short Spent Pleurotus ostreatus Substrate Has Potential for Managing Fusarium Wilt of Banana
title_sort spent pleurotus ostreatus substrate has potential for managing fusarium wilt of banana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620842/
https://www.ncbi.nlm.nih.gov/pubmed/34829233
http://dx.doi.org/10.3390/jof7110946
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