Cargando…

Revisiting the Hybridization Processes in the Triatoma brasiliensis Complex (Hemiptera, Triatominae): Reproductive Isolation between Triatoma petrocchiae and T. b. brasiliensis and T. lenti

SIMPLE SUMMARY: Although all triatomines are potential vectors of Chagas disease, there are species with greater or lesser vectorial importance. Therefore, the correct identification of triatomine species is essential for vector control programs. In general, triatomines are identified by external mo...

Descripción completa

Detalles Bibliográficos
Autores principales: Delgado, Luiza Maria Grzyb, de Oliveira, Jader, Ravazi, Amanda, Madeira, Fernanda Fernandez, dos Reis, Yago Visinho, Pinotti, Heloisa, de Oliveira, Ana Beatriz Bortolozo, Masarin, Isabella da Silva, Lilioso, Maurício, Folly-Ramos, Elaine, Galvão, Cleber, de Azeredo-Oliveira, Maria Tercília Vilela, da Rosa, João Aristeu, Alevi, Kaio Cesar Chaboli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621033/
https://www.ncbi.nlm.nih.gov/pubmed/34821815
http://dx.doi.org/10.3390/insects12111015
_version_ 1784605360022093824
author Delgado, Luiza Maria Grzyb
de Oliveira, Jader
Ravazi, Amanda
Madeira, Fernanda Fernandez
dos Reis, Yago Visinho
Pinotti, Heloisa
de Oliveira, Ana Beatriz Bortolozo
Masarin, Isabella da Silva
Lilioso, Maurício
Folly-Ramos, Elaine
Galvão, Cleber
de Azeredo-Oliveira, Maria Tercília Vilela
da Rosa, João Aristeu
Alevi, Kaio Cesar Chaboli
author_facet Delgado, Luiza Maria Grzyb
de Oliveira, Jader
Ravazi, Amanda
Madeira, Fernanda Fernandez
dos Reis, Yago Visinho
Pinotti, Heloisa
de Oliveira, Ana Beatriz Bortolozo
Masarin, Isabella da Silva
Lilioso, Maurício
Folly-Ramos, Elaine
Galvão, Cleber
de Azeredo-Oliveira, Maria Tercília Vilela
da Rosa, João Aristeu
Alevi, Kaio Cesar Chaboli
author_sort Delgado, Luiza Maria Grzyb
collection PubMed
description SIMPLE SUMMARY: Although all triatomines are potential vectors of Chagas disease, there are species with greater or lesser vectorial importance. Therefore, the correct identification of triatomine species is essential for vector control programs. In general, triatomines are identified by external morphological characters. However, some species are very similar or even morphologically identical, making the use of complementary analyses for the correct identification of species important. For this reason, this study focused on the use of experimental crosses to assess the specific status of species of the Triatoma brasiliensis species complex. The crosses did not result in hybrids, demonstrating that there are pre-zygotic reproductive barriers installed between T. petrocchiae and the other species of the T. brasiliensis complex (which confirms the specific status of the species according to the biological species concept). On the basis of the results above, we demonstrated that T. petrocchiae is reproductively isolated from T. b. brasiliensis and T. lenti. Furthermore, we suggest that T. petrocchiae is the species most derived from the T. brasiliensis complex. ABSTRACT: Triatoma petrocchiae is a species morphologically similar to T. b. brasiliensis (which resulted in a synonymization event); despite this similarity, genetic, morphological, and experimental crossbreeding studies confirmed the specific status of T. petrocchiae. Considering that both species have been reported living in sympatry and that, for a long time, most species of the T. brasiliensis complex were considered only chromatic variants of T. b. brasiliensis, we carried out experimental crosses between T. b. brasiliensis and T. petrocchiae (to confirm whether these species are reproductively isolated) and between T. lenti and T. petrocchiae (to assess whether T. petrocchiae also presents prezygotic isolation with the other species of the T. brasiliensis complex). Reciprocal experimental crosses were conducted, and weekly, the eggs were collected, counted, and separated in new containers to assess the hatch rate. Neither cross resulted in hybrids, demonstrating that there are pre-zygotic reproductive barriers installed between T. petrocchiae and the other species of the T. brasiliensis complex. On the basis of the results above, we demonstrated that T. petrocchiae is reproductively isolated from T. b. brasiliensis and T. lenti. Furthermore, we suggest that T. petrocchiae is the species most derived from the T. brasiliensis complex.
format Online
Article
Text
id pubmed-8621033
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-86210332021-11-27 Revisiting the Hybridization Processes in the Triatoma brasiliensis Complex (Hemiptera, Triatominae): Reproductive Isolation between Triatoma petrocchiae and T. b. brasiliensis and T. lenti Delgado, Luiza Maria Grzyb de Oliveira, Jader Ravazi, Amanda Madeira, Fernanda Fernandez dos Reis, Yago Visinho Pinotti, Heloisa de Oliveira, Ana Beatriz Bortolozo Masarin, Isabella da Silva Lilioso, Maurício Folly-Ramos, Elaine Galvão, Cleber de Azeredo-Oliveira, Maria Tercília Vilela da Rosa, João Aristeu Alevi, Kaio Cesar Chaboli Insects Communication SIMPLE SUMMARY: Although all triatomines are potential vectors of Chagas disease, there are species with greater or lesser vectorial importance. Therefore, the correct identification of triatomine species is essential for vector control programs. In general, triatomines are identified by external morphological characters. However, some species are very similar or even morphologically identical, making the use of complementary analyses for the correct identification of species important. For this reason, this study focused on the use of experimental crosses to assess the specific status of species of the Triatoma brasiliensis species complex. The crosses did not result in hybrids, demonstrating that there are pre-zygotic reproductive barriers installed between T. petrocchiae and the other species of the T. brasiliensis complex (which confirms the specific status of the species according to the biological species concept). On the basis of the results above, we demonstrated that T. petrocchiae is reproductively isolated from T. b. brasiliensis and T. lenti. Furthermore, we suggest that T. petrocchiae is the species most derived from the T. brasiliensis complex. ABSTRACT: Triatoma petrocchiae is a species morphologically similar to T. b. brasiliensis (which resulted in a synonymization event); despite this similarity, genetic, morphological, and experimental crossbreeding studies confirmed the specific status of T. petrocchiae. Considering that both species have been reported living in sympatry and that, for a long time, most species of the T. brasiliensis complex were considered only chromatic variants of T. b. brasiliensis, we carried out experimental crosses between T. b. brasiliensis and T. petrocchiae (to confirm whether these species are reproductively isolated) and between T. lenti and T. petrocchiae (to assess whether T. petrocchiae also presents prezygotic isolation with the other species of the T. brasiliensis complex). Reciprocal experimental crosses were conducted, and weekly, the eggs were collected, counted, and separated in new containers to assess the hatch rate. Neither cross resulted in hybrids, demonstrating that there are pre-zygotic reproductive barriers installed between T. petrocchiae and the other species of the T. brasiliensis complex. On the basis of the results above, we demonstrated that T. petrocchiae is reproductively isolated from T. b. brasiliensis and T. lenti. Furthermore, we suggest that T. petrocchiae is the species most derived from the T. brasiliensis complex. MDPI 2021-11-11 /pmc/articles/PMC8621033/ /pubmed/34821815 http://dx.doi.org/10.3390/insects12111015 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Delgado, Luiza Maria Grzyb
de Oliveira, Jader
Ravazi, Amanda
Madeira, Fernanda Fernandez
dos Reis, Yago Visinho
Pinotti, Heloisa
de Oliveira, Ana Beatriz Bortolozo
Masarin, Isabella da Silva
Lilioso, Maurício
Folly-Ramos, Elaine
Galvão, Cleber
de Azeredo-Oliveira, Maria Tercília Vilela
da Rosa, João Aristeu
Alevi, Kaio Cesar Chaboli
Revisiting the Hybridization Processes in the Triatoma brasiliensis Complex (Hemiptera, Triatominae): Reproductive Isolation between Triatoma petrocchiae and T. b. brasiliensis and T. lenti
title Revisiting the Hybridization Processes in the Triatoma brasiliensis Complex (Hemiptera, Triatominae): Reproductive Isolation between Triatoma petrocchiae and T. b. brasiliensis and T. lenti
title_full Revisiting the Hybridization Processes in the Triatoma brasiliensis Complex (Hemiptera, Triatominae): Reproductive Isolation between Triatoma petrocchiae and T. b. brasiliensis and T. lenti
title_fullStr Revisiting the Hybridization Processes in the Triatoma brasiliensis Complex (Hemiptera, Triatominae): Reproductive Isolation between Triatoma petrocchiae and T. b. brasiliensis and T. lenti
title_full_unstemmed Revisiting the Hybridization Processes in the Triatoma brasiliensis Complex (Hemiptera, Triatominae): Reproductive Isolation between Triatoma petrocchiae and T. b. brasiliensis and T. lenti
title_short Revisiting the Hybridization Processes in the Triatoma brasiliensis Complex (Hemiptera, Triatominae): Reproductive Isolation between Triatoma petrocchiae and T. b. brasiliensis and T. lenti
title_sort revisiting the hybridization processes in the triatoma brasiliensis complex (hemiptera, triatominae): reproductive isolation between triatoma petrocchiae and t. b. brasiliensis and t. lenti
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621033/
https://www.ncbi.nlm.nih.gov/pubmed/34821815
http://dx.doi.org/10.3390/insects12111015
work_keys_str_mv AT delgadoluizamariagrzyb revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT deoliveirajader revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT ravaziamanda revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT madeirafernandafernandez revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT dosreisyagovisinho revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT pinottiheloisa revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT deoliveiraanabeatrizbortolozo revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT masarinisabelladasilva revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT liliosomauricio revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT follyramoselaine revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT galvaocleber revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT deazeredooliveiramariaterciliavilela revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT darosajoaoaristeu revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti
AT alevikaiocesarchaboli revisitingthehybridizationprocessesinthetriatomabrasiliensiscomplexhemipteratriatominaereproductiveisolationbetweentriatomapetrocchiaeandtbbrasiliensisandtlenti