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The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens
Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants. However, using PCR-based methods for in-field diagno...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621059/ https://www.ncbi.nlm.nih.gov/pubmed/34834787 http://dx.doi.org/10.3390/plants10112424 |
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author | Ivanov, Aleksandr V. Safenkova, Irina V. Zherdev, Anatoly V. Dzantiev, Boris B. |
author_facet | Ivanov, Aleksandr V. Safenkova, Irina V. Zherdev, Anatoly V. Dzantiev, Boris B. |
author_sort | Ivanov, Aleksandr V. |
collection | PubMed |
description | Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants. However, using PCR-based methods for in-field diagnostics is a challenge and sometimes nearly impossible. With the advent of isothermal amplification methods, which provide amplification of nucleic acids at a certain temperature and do not require thermocyclic equipment, going beyond the laboratory has become a reality for molecular diagnostics. The amplification stage ceases to be limited by time and instruments. Challenges to solve involve finding suitable approaches for rapid and user-friendly plant preparation and detection of amplicons after amplification. Here, we summarize approaches for in-field diagnostics of phytopathogens based on different types of isothermal amplification and discuss their advantages and disadvantages. In this review, we consider a combination of isothermal amplification methods with extraction and detection methods compatible with in-field phytodiagnostics. Molecular diagnostics in out-of-lab conditions are of particular importance for protecting against viral, bacterial, and fungal phytopathogens in order to quickly prevent and control the spread of disease. We believe that the development of rapid, sensitive, and equipment-free nucleic acid detection methods is the future of phytodiagnostics, and its benefits are already visible. |
format | Online Article Text |
id | pubmed-8621059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86210592021-11-27 The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens Ivanov, Aleksandr V. Safenkova, Irina V. Zherdev, Anatoly V. Dzantiev, Boris B. Plants (Basel) Review Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants. However, using PCR-based methods for in-field diagnostics is a challenge and sometimes nearly impossible. With the advent of isothermal amplification methods, which provide amplification of nucleic acids at a certain temperature and do not require thermocyclic equipment, going beyond the laboratory has become a reality for molecular diagnostics. The amplification stage ceases to be limited by time and instruments. Challenges to solve involve finding suitable approaches for rapid and user-friendly plant preparation and detection of amplicons after amplification. Here, we summarize approaches for in-field diagnostics of phytopathogens based on different types of isothermal amplification and discuss their advantages and disadvantages. In this review, we consider a combination of isothermal amplification methods with extraction and detection methods compatible with in-field phytodiagnostics. Molecular diagnostics in out-of-lab conditions are of particular importance for protecting against viral, bacterial, and fungal phytopathogens in order to quickly prevent and control the spread of disease. We believe that the development of rapid, sensitive, and equipment-free nucleic acid detection methods is the future of phytodiagnostics, and its benefits are already visible. MDPI 2021-11-10 /pmc/articles/PMC8621059/ /pubmed/34834787 http://dx.doi.org/10.3390/plants10112424 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Ivanov, Aleksandr V. Safenkova, Irina V. Zherdev, Anatoly V. Dzantiev, Boris B. The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens |
title | The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens |
title_full | The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens |
title_fullStr | The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens |
title_full_unstemmed | The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens |
title_short | The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens |
title_sort | potential use of isothermal amplification assays for in-field diagnostics of plant pathogens |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621059/ https://www.ncbi.nlm.nih.gov/pubmed/34834787 http://dx.doi.org/10.3390/plants10112424 |
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