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Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil

Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and...

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Autores principales: Reis, Rosani Santos, Bonna, Isabel Cristina Fábregas, Antonio, Isabela Maria da Silva, Pereira, Sandro Antonio, do Nascimento, Carlos Roberto Sobrinho, Ferraris, Fausto Klabund, Brito-Santos, Fábio, Ferreira Gremião, Isabella Dib, Trilles, Luciana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621350/
https://www.ncbi.nlm.nih.gov/pubmed/34829267
http://dx.doi.org/10.3390/jof7110980
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author Reis, Rosani Santos
Bonna, Isabel Cristina Fábregas
Antonio, Isabela Maria da Silva
Pereira, Sandro Antonio
do Nascimento, Carlos Roberto Sobrinho
Ferraris, Fausto Klabund
Brito-Santos, Fábio
Ferreira Gremião, Isabella Dib
Trilles, Luciana
author_facet Reis, Rosani Santos
Bonna, Isabel Cristina Fábregas
Antonio, Isabela Maria da Silva
Pereira, Sandro Antonio
do Nascimento, Carlos Roberto Sobrinho
Ferraris, Fausto Klabund
Brito-Santos, Fábio
Ferreira Gremião, Isabella Dib
Trilles, Luciana
author_sort Reis, Rosani Santos
collection PubMed
description Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and VNIV), and the C. gattii species complex comprises another four (VGI, VGII, VGIII, and VGIV) and the newly identified molecular type VGV. These major molecular types differ in their epidemiological and ecological features, clinical presentations, and therapeutic outcomes. Generally, the most common isolated types are VNI, VGI, and VGII. The epidemiological profile of cryptococcosis in domestic cats is poorly studied and cats can be the sentinels for human infections. Therefore, the present study aimed to determine the molecular characterization of Cryptococcus spp. isolated from domestic cats and their dwellings in the metropolitan area of Rio de Janeiro, Brazil. A total of 36 Cryptococcus spp. strains, both clinical and environmental, from 19 cats were subtyped using multilocus sequence typing (MLST). The ploidy was identified using flow cytometry and the mating type was determined through amplification with specific pheromone primers. All strains were mating type alpha and 6/36 were diploid (all VNII). Most isolates (63.88%) were identified as VNII, a rare molecular type, leading to the consideration that this genotype is more likely related to skin lesions, since there was a high percentage (68.75%) of cats with skin lesions, which is also considered rare. Further studies regarding the molecular epidemiology of cryptococcosis in felines are still needed to clarify the reason for the large proportion of the rare molecular type VNII causing infections in cats.
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spelling pubmed-86213502021-11-27 Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil Reis, Rosani Santos Bonna, Isabel Cristina Fábregas Antonio, Isabela Maria da Silva Pereira, Sandro Antonio do Nascimento, Carlos Roberto Sobrinho Ferraris, Fausto Klabund Brito-Santos, Fábio Ferreira Gremião, Isabella Dib Trilles, Luciana J Fungi (Basel) Article Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and VNIV), and the C. gattii species complex comprises another four (VGI, VGII, VGIII, and VGIV) and the newly identified molecular type VGV. These major molecular types differ in their epidemiological and ecological features, clinical presentations, and therapeutic outcomes. Generally, the most common isolated types are VNI, VGI, and VGII. The epidemiological profile of cryptococcosis in domestic cats is poorly studied and cats can be the sentinels for human infections. Therefore, the present study aimed to determine the molecular characterization of Cryptococcus spp. isolated from domestic cats and their dwellings in the metropolitan area of Rio de Janeiro, Brazil. A total of 36 Cryptococcus spp. strains, both clinical and environmental, from 19 cats were subtyped using multilocus sequence typing (MLST). The ploidy was identified using flow cytometry and the mating type was determined through amplification with specific pheromone primers. All strains were mating type alpha and 6/36 were diploid (all VNII). Most isolates (63.88%) were identified as VNII, a rare molecular type, leading to the consideration that this genotype is more likely related to skin lesions, since there was a high percentage (68.75%) of cats with skin lesions, which is also considered rare. Further studies regarding the molecular epidemiology of cryptococcosis in felines are still needed to clarify the reason for the large proportion of the rare molecular type VNII causing infections in cats. MDPI 2021-11-18 /pmc/articles/PMC8621350/ /pubmed/34829267 http://dx.doi.org/10.3390/jof7110980 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reis, Rosani Santos
Bonna, Isabel Cristina Fábregas
Antonio, Isabela Maria da Silva
Pereira, Sandro Antonio
do Nascimento, Carlos Roberto Sobrinho
Ferraris, Fausto Klabund
Brito-Santos, Fábio
Ferreira Gremião, Isabella Dib
Trilles, Luciana
Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil
title Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil
title_full Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil
title_fullStr Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil
title_full_unstemmed Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil
title_short Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil
title_sort cryptococcus neoformans vnii as the main cause of cryptococcosis in domestic cats from rio de janeiro, brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621350/
https://www.ncbi.nlm.nih.gov/pubmed/34829267
http://dx.doi.org/10.3390/jof7110980
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