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Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil
Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621350/ https://www.ncbi.nlm.nih.gov/pubmed/34829267 http://dx.doi.org/10.3390/jof7110980 |
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author | Reis, Rosani Santos Bonna, Isabel Cristina Fábregas Antonio, Isabela Maria da Silva Pereira, Sandro Antonio do Nascimento, Carlos Roberto Sobrinho Ferraris, Fausto Klabund Brito-Santos, Fábio Ferreira Gremião, Isabella Dib Trilles, Luciana |
author_facet | Reis, Rosani Santos Bonna, Isabel Cristina Fábregas Antonio, Isabela Maria da Silva Pereira, Sandro Antonio do Nascimento, Carlos Roberto Sobrinho Ferraris, Fausto Klabund Brito-Santos, Fábio Ferreira Gremião, Isabella Dib Trilles, Luciana |
author_sort | Reis, Rosani Santos |
collection | PubMed |
description | Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and VNIV), and the C. gattii species complex comprises another four (VGI, VGII, VGIII, and VGIV) and the newly identified molecular type VGV. These major molecular types differ in their epidemiological and ecological features, clinical presentations, and therapeutic outcomes. Generally, the most common isolated types are VNI, VGI, and VGII. The epidemiological profile of cryptococcosis in domestic cats is poorly studied and cats can be the sentinels for human infections. Therefore, the present study aimed to determine the molecular characterization of Cryptococcus spp. isolated from domestic cats and their dwellings in the metropolitan area of Rio de Janeiro, Brazil. A total of 36 Cryptococcus spp. strains, both clinical and environmental, from 19 cats were subtyped using multilocus sequence typing (MLST). The ploidy was identified using flow cytometry and the mating type was determined through amplification with specific pheromone primers. All strains were mating type alpha and 6/36 were diploid (all VNII). Most isolates (63.88%) were identified as VNII, a rare molecular type, leading to the consideration that this genotype is more likely related to skin lesions, since there was a high percentage (68.75%) of cats with skin lesions, which is also considered rare. Further studies regarding the molecular epidemiology of cryptococcosis in felines are still needed to clarify the reason for the large proportion of the rare molecular type VNII causing infections in cats. |
format | Online Article Text |
id | pubmed-8621350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86213502021-11-27 Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil Reis, Rosani Santos Bonna, Isabel Cristina Fábregas Antonio, Isabela Maria da Silva Pereira, Sandro Antonio do Nascimento, Carlos Roberto Sobrinho Ferraris, Fausto Klabund Brito-Santos, Fábio Ferreira Gremião, Isabella Dib Trilles, Luciana J Fungi (Basel) Article Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and VNIV), and the C. gattii species complex comprises another four (VGI, VGII, VGIII, and VGIV) and the newly identified molecular type VGV. These major molecular types differ in their epidemiological and ecological features, clinical presentations, and therapeutic outcomes. Generally, the most common isolated types are VNI, VGI, and VGII. The epidemiological profile of cryptococcosis in domestic cats is poorly studied and cats can be the sentinels for human infections. Therefore, the present study aimed to determine the molecular characterization of Cryptococcus spp. isolated from domestic cats and their dwellings in the metropolitan area of Rio de Janeiro, Brazil. A total of 36 Cryptococcus spp. strains, both clinical and environmental, from 19 cats were subtyped using multilocus sequence typing (MLST). The ploidy was identified using flow cytometry and the mating type was determined through amplification with specific pheromone primers. All strains were mating type alpha and 6/36 were diploid (all VNII). Most isolates (63.88%) were identified as VNII, a rare molecular type, leading to the consideration that this genotype is more likely related to skin lesions, since there was a high percentage (68.75%) of cats with skin lesions, which is also considered rare. Further studies regarding the molecular epidemiology of cryptococcosis in felines are still needed to clarify the reason for the large proportion of the rare molecular type VNII causing infections in cats. MDPI 2021-11-18 /pmc/articles/PMC8621350/ /pubmed/34829267 http://dx.doi.org/10.3390/jof7110980 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Reis, Rosani Santos Bonna, Isabel Cristina Fábregas Antonio, Isabela Maria da Silva Pereira, Sandro Antonio do Nascimento, Carlos Roberto Sobrinho Ferraris, Fausto Klabund Brito-Santos, Fábio Ferreira Gremião, Isabella Dib Trilles, Luciana Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil |
title | Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil |
title_full | Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil |
title_fullStr | Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil |
title_full_unstemmed | Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil |
title_short | Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil |
title_sort | cryptococcus neoformans vnii as the main cause of cryptococcosis in domestic cats from rio de janeiro, brazil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621350/ https://www.ncbi.nlm.nih.gov/pubmed/34829267 http://dx.doi.org/10.3390/jof7110980 |
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