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Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation
There is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621608/ https://www.ncbi.nlm.nih.gov/pubmed/34830319 http://dx.doi.org/10.3390/ijms222212437 |
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author | Ogura, Keiji Ayabe, Yoshiko Harada, Chihiro Tanaka, Ignacia Braga Tanaka, Satoshi Komura, Jun-ichiro |
author_facet | Ogura, Keiji Ayabe, Yoshiko Harada, Chihiro Tanaka, Ignacia Braga Tanaka, Satoshi Komura, Jun-ichiro |
author_sort | Ogura, Keiji |
collection | PubMed |
description | There is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridization technology. We observed that the number of the mice with de novo copy number variations (specifically, deletions) was significantly increased in the offspring of C57BL/6J male mice exposed to 20 mGy/day gamma-rays for 400 days (total dose: 8000 mGy), as compared to non-irradiated controls. We did not detect any difference in the size of the de novo deletions between the irradiated and the non-irradiated groups. An analysis of the life span of the offspring suggested a possibility that de novo copy-number variations may be associated with shorter life spans. |
format | Online Article Text |
id | pubmed-8621608 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-86216082021-11-27 Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation Ogura, Keiji Ayabe, Yoshiko Harada, Chihiro Tanaka, Ignacia Braga Tanaka, Satoshi Komura, Jun-ichiro Int J Mol Sci Article There is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridization technology. We observed that the number of the mice with de novo copy number variations (specifically, deletions) was significantly increased in the offspring of C57BL/6J male mice exposed to 20 mGy/day gamma-rays for 400 days (total dose: 8000 mGy), as compared to non-irradiated controls. We did not detect any difference in the size of the de novo deletions between the irradiated and the non-irradiated groups. An analysis of the life span of the offspring suggested a possibility that de novo copy-number variations may be associated with shorter life spans. MDPI 2021-11-18 /pmc/articles/PMC8621608/ /pubmed/34830319 http://dx.doi.org/10.3390/ijms222212437 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ogura, Keiji Ayabe, Yoshiko Harada, Chihiro Tanaka, Ignacia Braga Tanaka, Satoshi Komura, Jun-ichiro Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
title | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
title_full | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
title_fullStr | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
title_full_unstemmed | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
title_short | Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation |
title_sort | increased frequency of copy number variations revealed by array comparative genomic hybridization in the offspring of male mice exposed to low dose-rate ionizing radiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621608/ https://www.ncbi.nlm.nih.gov/pubmed/34830319 http://dx.doi.org/10.3390/ijms222212437 |
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